Secondary Antibodies

Rat IgM (Mu chain specific) mouse monoclonal antibody, clone M 2A1, PE

Applications Flow Cytometry: (< / = 0.1 µg/10e6 cells) and Immunofluorescence Microscopy: Identification and enumeration of IgM+ cells.
FLISA: < / = 1.0 µg/ml
Reactivities Rat
Conjugation PE

Rat IgG2a (gamma 2a chain specific) mouse monoclonal antibody, clone 2A 8F4, PE

Applications Flow Cytometry: < / = 0.1 µg/10e6 cells.
Immunofluorescence Microscopy: Identification and enumeration of IgG2a+ cells.
Reactivities Rat
Conjugation PE

Rat IgG2c (gamma 2c chain specific) mouse monoclonal antibody, clone 2C 8F1, PE

Applications Flow Cytometry: FITC and BIOT conjugates ≤1 µg/10e6 cells.
Immunofluorescence Microscopy: Identification and enumeration of IgG2c+ cells.
ELISA.
Reactivities Rat
Conjugation PE

Rat IgG2b (Gamma-2b chain specific) mouse monoclonal antibody, clone 2B 10A8, PE

Applications Flow Cytometry: < / = 0.02 µg/10e6 cells.
Immunofluorescence Microscopy: Identification and enumeration of IgG2b+ cells.
Reactivities Rat
Conjugation PE

Rat IgG1 (gamma 1 chain specific) mouse monoclonal antibody, clone G1 7E7, PE

Applications Flow Cytometry: < / = 0.02 µg/10e6 cells.
Immunofluorescence Microscopy: Identification and enumeration of IgG1+ cells.
Reactivities Rat
Conjugation PE

Rat IgG1 (gamma 1 chain specific) mouse monoclonal antibody, clone G1 1C5, PE

Applications Flow Cytometry: < / = 0.02 µg/10e6 cells.
Immunofluorescence Microscopy: Identification and enumeration of IgG1+ cells.
Reactivities Rat
Conjugation PE

Rat IgG (H+L chain), F(ab)2 Fragment, adsorbed goat polyclonal antibody, PE

Applications Flow cytometry (1/5 - 1/10): use 50 µl of the suggested working dilution to label 10e6 cells in 100 µl.
Reactivities Rat
Conjugation PE

Rat IgM (Mu chain specific) goat polyclonal antibody, PE

Applications Suitable for immunomicroscopy and flow cytometry as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.
Recommended dilutions:
The maximum amount of reagent required to stain 1 x 10e6 cells in flow cytometry is approximately 1.0 µg of antibody conjugate. Lesser amounts of reagent may be sufficient for staining.
As a general guideline dilutions of 1:50 to 1:200 should be suitable for most applications.
Reactivities Rat
Conjugation PE

Rat IgG (F(ab)2 Fragment) Fc specific goat polyclonal antibody, PE

Applications Suitable for Immunomicroscopy (1:100-1:250) and Flow cytometry (1:100-1:250) or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.
Reactivities Rat
Conjugation PE

Rat IgG (H+L chain) F(ab')2 Fragment donkey polyclonal antibody, PE

Applications Suitable for Immunomicroscopy (1:100-1:200) and Flow Cytometry (1:100-1:200) or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.
Reactivities Rat
Conjugation PE

Rat IgG (H+L chain), F(ab)2 Fragment goat polyclonal antibody, PE

Applications Suitable for Immunomicroscopy and Flow Cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.
The maximum amount of reagent required to stain 1 x 10e6 cells in Flow Cytometry is approximately 1.0 µg of antibody conjugate. Lesser amounts of reagent may be sufficient for staining. As a general guideline dilutions of 1:100 to 1:250 should be suitable for most applications.
Reactivities Rat
Conjugation PE