Secondary Antibodies

Monkey IgM (Mu chain specific) goat polyclonal antibody, Biotin

Applications Suitable for Immunoblotting, ELISA, Immunohistochemistry, immunomicroscopy as well as other antibody based assays using streptavidin or avidin conjugates requiring lot-to-lot consistency.
Recommended Dilutions:
ELISA: 1/20,000-1/300,000.
Western Blot: 1/2,000-1/10,000.
Immunohistochemistry: 1/1,000-1/5,000.
This product has been assayed against 1.0 ug of Monkey IgM in a standard capture ELISA using Peroxidase Conjugated Streptavidin and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1/5,000 to 1/20,000 of the concentration is suggested for this product.
Reactivities Monkey
Conjugation Biotin

Monkey IgG (gamma chain specific) goat polyclonal antibody, Aff - Purified

Applications Suitable for Immunoprecipitation, Immunodiffusion, conjugation and most immunological methods requiring lot-to-lot consistency, high titer and specificity.
ELISA: 1:20,000 - 1:100,000 dilution.
Western Blot: 1:2000 - 1:10,000
Immunohistochemistry: 1:1000-1:5000
Reactivities Monkey
Conjugation Unconjugated

Human IgM (Fc5 mu specific), F(ab)2 Fragment goat polyclonal antibody, AP

Applications Suitable for Immunoblotting (Western or Dot blot), ELISA and immunohistochemistry as well as other phosphatase-antibody based enzymatic assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.
Recommended Dilutions:
ELISA: 1/7,500-1/30,000.
Western Blot: 1/1000-1/5000.
Immunohistochemistry: 1/200-1/1000.
Note: This product has been assayed against 1.0 µg of Human IgM in a standard capture ELISA using pNPP p-nitrophenyl phosphate as a substrate for 30 minutes at room temperature. A working dilution of 1/5,000 to 1/50,000 is suggested for this product.
Reactivities Human
Conjugation AP

Rabbit IgG (H+L chain, adsorbed) goat polyclonal antibody, FITC

Applications Suitable for immunomicroscopy (1:1,000-1:5,000) and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
ELISA : 1/10,000-1/50,000.
Reactivities Rabbit
Conjugation FITC

Sheep IgG (H+L chain) rabbit polyclonal antibody, Aff - Purified

Applications Suitable for Immunoprecipitation, Immunodiffusion, conjugation and most immunological methods requiring lot-to-lot consistency, high titer and specificity.
Recommended dilutions:
ELISA : 1 :20,000-1 :100,000
Western blot : 1 :2,000-1 :10,000
Immunohistochemistry : 1 :1,000-1 :5,000.
Reactivities Sheep
Conjugation Unconjugated

Mouse IgG2a (gamma 2a chain specific) rabbit polyclonal antibody, Biotin

Applications Immunoblotting, ELISA, Immunohistochemistry, Immunomicroscopy as well as other antibody based assay using streptavidin or avidin conjugates requiring lot-to-lot consistency.
Recommended Dilutions: This product has been assayed against 1.0 mg of Mouse IgG2a in a standard capture ELISA using Peroxidase Conjugated Streptavidin and ABTS (2,2'-azino-bis-[3-ethylbenthiozoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:30,000 to 1:150,000 of the reconstitution concentration is suggested for this product.
Reactivities Mouse
Conjugation Biotin

Mouse IgG1 (gamma 1 chain specific) rabbit polyclonal antibody, Aff - Purified

Applications Suitable for Immunoprecipitation, Immunodiffusion, conjugation and most immunological methods requiring lot-to-lot consistency, high titer and specificity.
Recommended Dilutions:
ELISA: 1/20,000-1/100,000.
Western Blot: 1/2,000-1/10,000.
Immunohistochemistry: 1/1,000-1/5,000.
Reactivities Mouse
Conjugation Unconjugated

Rat IgG (H+L chain, adsorbed) goat polyclonal antibody, Biotin

Applications Suitable for Immunoblotting (1/2,000-1/10,000), ELISA, Immunohistochemistry (1/1,000-1/5,000), Immunomicroscopy as well as other antibody based assays using streptavidin or avidin conjugates requiring lot-to-lot consistency.
Recommended Dilutions: This product has been assayed against 1.0 µg of Rat IgG in a standard capture ELISA using Peroxidase Conjugated Streptavidin and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:20,000 to 1:100,000 of the reconstitution concentration is suggested for this product.
Reactivities Rat
Conjugation Biotin

Goat IgG (H+L chain), F(ab)2 Fragment, adsorbed rabbit polyclonal antibody, FITC

Applications Suitable for Immunomicroscopy and Flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.
Recommended Dilution(s):
FLISA: 1/10,000 - 1/50,000
IF Microscopy: 1/1000- 1/5000
Reactivities Goat
Conjugation FITC

Human IgG (gamma chain specific) rabbit polyclonal antibody, TRITC

Applications Suitable for Immunomicroscopy and Flow Cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent Western blotting. Also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
Recommended Dilutions:
FLISA: 1/10,000-1/50,000.
IF Microscopy: 1/1,000-1/5,000.
Flow Cytometry: 1/500-1/2,500.
Reactivities Human
Conjugation TRITC

Rat IgG (H+L chain) rabbit polyclonal antibody, Biotin

Applications Suitable for Immunoblotting, ELISA, Immunohistochemistry, Immunomicroscopy as well as other antibody based assays using streptavidin or avidin conjugates requiring lot-to-lot consistency.
Recommended Dilutions:
ELISA: 1/500,000.
Western blot: 1/2,000-1/10,000.
Immunohistochemistry: 1/1,000-1/5,000.
Note: When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment.
Reactivities Rat
Conjugation Biotin

Mouse IgG (F(ab)2 specific), F(ab)2 Fragment, adsorbed goat polyclonal antibody, HRP

Applications Suitable for Immunoblotting (Western or dot blot), ELISA, Immunoperoxidase electron microscopy and Immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.
Recommended Dilutions: This product has been assayed against 1.0 ug of Mouse IgG in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature.

ELISA: 1/130,000
Immunohistochemistry: 1/500-1/2500
Western Blot: 1/2000-1/20,000

Reactivities Mouse
Conjugation HRP

Human IgG (H+L chain) chicken polyclonal antibody, HRP

Applications Suitable for Immunoblotting (1/1,000-1/5,000, Western or Dot blot), ELISA (1/10,000-1/40,000), Immunoperoxidase electron microscopy and Immunohistochemistry (1/500-1/2,500) as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency.
Reactivities Human
Conjugation HRP

Goat IgG (Fc specific) rabbit polyclonal antibody, HRP

Applications Western Blot/Dot Blot: 1/1,000-1/10,000 or Dot blot.
Immunohistochemistry: 1/500-1/2,500.
ELISA: This product has been assayed against 1.0 µg of Goat IgG in a standard capture ELISA using TMB as a substrate for 30 minutes at room temperature. A working dilution of 1/4,000 to 1/16,000 is suggested for this product.
Reactivities Goat
Conjugation HRP

Mouse IgG (H+L chain), F(ab)2 Fragment rabbit polyclonal antibody, HRP

Applications ELISA: 1/500 - 1/1000.
Western Blot: 1/2000 - 1/5000.
Immunohistochemistry on frozen, paraffin and resin sections: 1/50.
Reactivities Mouse
Conjugation HRP