Secondary Antibodies

Human IgG4 (pFC) mouse monoclonal antibody, clone HP6023 (also known as HO6023), AP, Purified

Applications ELISA: 1/1000-1/2000 (See also Ref.2-9).
FLISA.
Applications Reported in Literature:
Flow Cytometry (Ref.15,18,19).
Immunohistochemistry on Frozen Sections (Ref.10).
Immunohistochemistry on Paraffin Sections (Ref.5,7,10,11).
Immunocytochemistry (Ref.12).
Immunoblotting (Ref.3,13-16).
Microarray (Ref.17).
Multiplex (Ref.2,20-22).
Purification (Ref.15).
Reactivities Human
Conjugation AP

IGHG4 mouse monoclonal antibody, clone HP6025, AP, Purified

Applications ELISA: 1/1000-1/2000 (2-9).
Immunohistochemistry on Frozen Sections: reported in literature (5,7,10,11).
Immunohistochemistry on Paraffin Sections: reported in literature (10).
Flow Cytometry:
reported in literature (15, 18, 19).
Immunocytochemistry: reported in literature (12).
Western Blot: reported in literature (3,13-16).
Reactivities Human
Conjugation AP

Human IgE goat polyclonal antibody, AP

Applications ELISA: 1/1000-1/10000.
Western Blot: 1/1000-1/5000.
Reactivities Human, Monkey
Conjugation AP

Human IgG3 (Hinge Region) mouse monoclonal antibody, clone HP6050, AP

Applications ELISA: 1/500-1/1,000.
Western blotting.
Dot- and Slot-Immunoblotting
Immunohistochemistry on Frozen Sections.
Reactivities Human
Conjugation AP

Human IgG (F(ab)2 specific) rabbit polyclonal antibody, AP

Applications Suitable for Immunoblotting (Western or Dot blot), ELISA and Immunohistochemistry as well as other phosphatase-antibody based enzymatic assays requiring lot-to-lot consistency.
Recommended Dilutions: This product has been assayed against 1.0 ug of Human IgG in a standard capture ELISA using pNPP p-nitrophenyl phosphate as a substrate for 30 minutes at room temperature. A working dilution of 1:3,000 to 1:15,000 is suggested for this product.
Reactivities Human
Conjugation AP

Human IgG (Fc specific) rabbit polyclonal antibody, AP

Applications Suitable for Immunoblotting (Western or Dot blot), ELISA and Immunohistochemistry as well as other phosphatase-antibody based enzymatic assays requiring lot-to-lot consistency.
Recommended Dilutions: This product has been assayed against 1.0 µg of Human IgG in a standard capture ELISA using pNPP p-nitrophenyl phosphate as a substrate for 30 minutes at room temperature. A working dilution of 1:1,600 to 1:16,000 of the reconstitution concentration is suggested for this product.
Reactivities Human
Conjugation AP