Secondary Antibodies

Rabbit Ig (IgG + IgM) rat monoclonal antibody, clone SB87a, Biotin

Applications ELISA: 1/5,000-1/20,000.
Flow Cytometry.
Reactivities Rabbit
Conjugation Biotin

Rat IgG1 (gamma 1 chain specific) mouse monoclonal antibody, clone G1 1C5, Biotin

Applications Flow Cytometry: < / = 0.3 µg/10e6 cells.
Immunofluorescence Microscopy: Identification and enumeration of IgG1+ cells.
ELISA: Can be used as Capture antibody.
Reactivities Rat
Conjugation Biotin

Rat IgG1 (gamma 1 chain specific) mouse monoclonal antibody, clone G1 7E7, Biotin

Applications Flow Cytometry: ≤ 1 µg/106 cells.
Immunofluorescence Microscopy: Identification and enumeration of IgG1+ cells.
ELISA: 1/5,000-1/20,000.
Reactivities Rat
Conjugation Biotin

Mouse IgA (alpha chain specific) rat monoclonal antibody, clone 11-44-2, Biotin

Applications Flow Cytometry: < / = 1 µg/10e6 cells.
Identification and enumeration of IgA+.
Immunofluorescence Microscopy: Identification and enumeration of IgA+.
ELISA.
Reactivities Mouse
Conjugation Biotin

Mouse IgM (Mu chain specific) rat monoclonal antibody, clone 1B4B1, Biotin

Applications Flow Cytometry: < / = 1 µg/10e6 cells.
Identification and enumeration of IgE+ cells.
Immunofluorescence Microscopy: Identification and enumeration of IgE+ cells .
ELISA.
Also used as a second step reagent for mouse IgM monoclonal antibodies.
Reactivities Mouse
Conjugation Biotin

Mouse IgG2a (gamma 2a chain specific) rat monoclonal antibody, clone SB84a, Biotin

Applications Flow Cytometry: Identification and enumeration of IgG2a+.
Immunofluorescence Microscopy: Identification and enumeration of IgG2a+.
ELISA.
Also used as a second step reagent for mouse IgG2a monoclonal antibodies.
Reactivities Mouse
Conjugation Biotin

Human IgG4 (pFC specific) mouse monoclonal antibody, clone NI 315 (HP 6206), Biotin

Applications To identify the presence of IgG4 in Human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as ELISA, immunoperoxidase staining of cytoplasmic IgG4, and Immunoblotting. As a second step an avidin or streptavidin conjugate of the customer’s choice have to be used.
General Recommended Dilutions:
Histochemical Use: 1/50-1/250.
ELISA: from 1/100 upwards.
Western blot: from 1/500 upwards.
Reactivities Human
Conjugation Biotin

Canine IgE mouse monoclonal antibody, clone E6-71A1, Biotin

Applications ELISA: The antibody may be used as a detection antibody in ELISA techniques in combination with SM1498P as capture antibody.
Western Blotting: Under reducing conditions, this antibody detects a band of approximately 62-65 kDa in samples of affinity purified Dog IgE.
Reactivities Canine, Human
Conjugation Biotin

Rat IgE mouse monoclonal antibody, clone MARE-1, Biotin

Applications ELISA: 5 µg/ml as detecting antibody.
Western Blot.
Reactivities Rat
Conjugation Biotin

Mouse IgE (heavy chain) rat monoclonal antibody, clone LO-ME-3, Biotin

Applications Useful as second antibody in Immunoassays, such as ELISA's (good binding on plastic) and Immunohistochemistry on Frozen Sections.
Reactivities Mouse
Conjugation Biotin

Mouse IgA (heavy chain) rat monoclonal antibody, clone LO-MA-7, Biotin, Purified

Applications Immunoassay.
ELISA: good binding on plastic.
Immunohistochemistry on frozen sections.
Reactivities Mouse
Conjugation Biotin

Mouse IgD (Delta heavy chain) rat monoclonal antibody, clone 11-26, Biotin

Applications Flow Cytometry: < / = 1µg/10e6 cells. (See Ref.1-5)
Reactivities Mouse
Conjugation Biotin

Human IgG2 (Fc specific) mouse monoclonal antibody, clone 31-7-4, Biotin

Applications ELISA: 1/5,000-1/20,000.
Immunofluorescence < / = 1 µg/10e6 cells.
Reactivities Human
Conjugation Biotin

Human IgD (Delta chain specific) mouse monoclonal antibody, clone IADB6, Biotin

Applications Indirect Immunofluorescent staining of IgD+ human B lymphocytes: ≤ 1 µg/106 cells.
ELISA: 1/5,000-1/10,000.
Reactivities Human
Conjugation Biotin