Secondary Antibodies

Human IgG1 (gamma 1 chain specific) mouse monoclonal antibody, clone 4E3, Purified

Applications ELISA: ≤ 5 µg/ml (Ref.1)
Immunofluorescence: ≤ 1 µg/10e6 cells.
Flow Cytometry.
Reactivities Human
Conjugation Unconjugated

Chicken IgM (Mu heavy chain specific) mouse monoclonal antibody, clone M-1, Purified

Applications Flow Cytometry.
Immunoprecipitation. (Ref.1)
Reactivities Chicken
Conjugation Unconjugated

Rabbit IgG mouse monoclonal antibody, clone 2A9, Purified

Applications ELISA: 1/4,000-1/8,000.
Flow Cytometry.
FLISA.
Reactivities Rabbit
Conjugation Unconjugated

Mouse IgM (Mu chain specific), F(ab)2 Fragment rat monoclonal antibody, clone SB73a, Purified

Applications Flow Cytometry: < / = 1 µg/10e6 cells.
Identification and enumeration of IgM+.
Immunofluorescence Microscopy: Identification and enumeration of IgM+.
ELISA: < / = 1 µg/ml.
Also used as a second step reagent for mouse IgM monoclonal antibodies.
Reactivities Mouse
Conjugation Unconjugated

Feline IgG mouse monoclonal antibody, clone GPB2-2B1, Purified

Applications ELISA: This antibody can bind to virus-bound immune IgG and can therefore detect anti-FeLV, FIV, FCV, FHV, FCoV/FIPV and FPV IgG activity in ELISA.
Western Blot: This antibody detects a band of approximately 50.0kDa on purified Feline IgG under reducing conditions.
Reactivities Feline
Conjugation Unconjugated

Human IgG (Fc CH2 Domain specific) mouse monoclonal antibody, clone 8A4, Purified

Applications ELISA.
Flow cytometry (1:50/1:100): use 10 µl of the suggested working dilution to label 10e6 cells in 100 µl.
Reactivities Human
Conjugation Unconjugated

Monkey IgA mouse monoclonal antibody, clone IgA5-3B, Purified

Applications ELISA.
Western Blot: Detects a band of approximately 55kDa under reducing conditions.
Reactivities Feline, Monkey
Conjugation Unconjugated

Human IgE mouse monoclonal antibody, clone 4G7, Purified

Applications ELISA.
Reactivities Human
Conjugation Unconjugated

Human IgE mouse monoclonal antibody, clone B537M, Purified

Applications ELISA.
Reactivities Human
Conjugation Unconjugated

Human IgE mouse monoclonal antibody, clone 090-11348, Purified

Applications ELISA.
Recommended antibody pair for Sandwich Immunoassay:
Capture / Detection
AM00919PU-N / AM00918PU-N
Reactivities Human
Conjugation Unconjugated

Human IgM (Mu chain, Fab-Region) mouse monoclonal antibody, clone B1545M, Purified

Applications
This antibody can pair with itself in ELISA, EIA.
Can be used for specific IgM antibodies detection and for total IgM level detection.
Can also be used as a capture antibody for total IgM level detection.
Reactivities Human
Conjugation Unconjugated

Human IgA mouse monoclonal antibody, clone DA43D3, Purified

Applications Suitable for Immunohistochemistry on Frozen (1 µg/ml, 1/400) and Paraffin Embedded Sections. Proteinase K pretreatment for antigen retrieval is recommended.
Suggested Positive Control: Human tonsil.
Reactivities Human
Conjugation Unconjugated

Human IgM mouse monoclonal antibody, clone DM3A3, Purified

Applications Immunohistochemistry on frozen sections: 1-2 µg/ml (1:200 - 1:400).
Immunohistochemistry on paraffin sections: Proteinase K pretreatment for antigen retrieval is recommended.
Suggested positive control: Human tonsil.
Reactivities Human
Conjugation Unconjugated

Human IgA (secretory) mouse monoclonal antibody, clone B781M, Purified

Applications ELISA: 1/20,000 dilution will yield > 1.0 O.D. using Alkaline Phosphatase conjugated Rabbit anti-Mouse Ig.
Reactivities Human
Conjugation Unconjugated

Human IgM mouse monoclonal antibody, clone 101, Purified

Applications ELISA.
Reactivities Human
Conjugation Unconjugated