Secondary Antibodies

Human IgA mouse monoclonal antibody, clone NI 69 (A89-034) and NI 184 (A89-035), Ascites

Applications To identify the presence of IgA in Human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as Radioimmunoassay, ELISA, Indirect Immunoperoxidase and Immunofluorescence staining of cytoplasmic IgA, Haemagglutination and Immunoblotting.
General Recommended Dilutions:
Histochemical Use: 1/50-1/200.
ELISA: from 1/500 upwards.
Western blot: from 1/1000 upwards.
Reactivities Human
Conjugation Unconjugated

hIgG1 and hIgG2 and hIgG3 F(ab')2 Fragment Specific Rabbit monoclonal antibody,OTIR4H6

Applications WB 1:2000, ELISA 1:5000-1:20000
Reactivities Human
Conjugation Unconjugated

【特别福利】购一抗正装产品(≥50ul或≥50ug),免费领10ul抗体试用装

hIgG Kappa light chain Specific Rabbit monoclonal antibody, OTIR1G7

Applications WB 1:2000, ELISA 1:5000-1:20000
Reactivities Human
Conjugation Unconjugated

【特别福利】购一抗正装产品(≥50ul或≥50ug),免费领10ul抗体试用装

Human IgG2 (Hinge Region) mouse monoclonal antibody, clone 3C7, Purified

Applications ELISA.
Reactivities Human
Conjugation Unconjugated

Human IgM (Mu chain, Fab-Region) mouse monoclonal antibody, clone UHB, Purified

Applications ELISA: ≤ 1 µg/ml.
Flow Cytometry: ≤ 1 µg/106 cells.
Immunofluorescence: ≤ 1 µg/ml.
Human B Cell Stimulation: ≤ 10 µg/ml.
Reactivities Human
Conjugation Unconjugated

Human IgE mouse monoclonal antibody, clone BE5, Purified

Applications ELISA.
Flow Cytometry: 5-10 µg/ml.
Reactivities Human
Conjugation Unconjugated

Human IgA (secretory) mouse monoclonal antibody, clone SC-05, Purified

Applications Western blot.
Immunoprecipitation.
Immunohistochemistry on Paraffin Sections.
Immunohistochemistry on Frozen Sections.

Examples of positive Human tissues: Secretory mucosa of gastrointestinal and respiratory tract, epithelia of salivary glands, endometrium, endocervix, kidney, prostate, mammary gland.
Reactivities Human
Conjugation Unconjugated

Human IgM (Fc-region) mouse monoclonal antibody, clone CH2, Purified

Applications Western blot. Recommended dilution: 1 μg/ml.
Flow cytometry: Recommended dilution: 1-4 μg/ml, extracellular and intracellular staining.
Immunocytochemistry.
ELISA.
Reactivities Human
Conjugation Unconjugated

Human IgE mouse monoclonal antibody, clone 904, Purified

Applications ELISA.
Reactivities Human
Conjugation Unconjugated

Human IgG (Fd region) mouse monoclonal antibody, clone HP6045, Purified

Applications ELISA (both capture and detection).
Western Blot.
Radioimmunoassay
Reactivities Human
Conjugation Unconjugated

Human IgG (F(ab)2 specific) mouse monoclonal antibody, clone 4A11, Purified

Applications ELISA.
Western blotting.
Flow Cytometry.

Has been described for previous lots to work in Immunofluorescence.
Reactivities Human
Conjugation Unconjugated

Human IgG1 (Fc specific) mouse monoclonal antibody, clone NI 132 (HP 6186), Purified

Applications To identify the presence of IgG1 in Human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as ELISA, Indirect Immunoperoxidase and Indirect Immunofluorescence staining of cytoplasmic IgG1, and Immunoblotting.
General Recommended Dilutions:
Histochemical Use: 1/50-1/200.
ELISA: from 1/500 upwards.
Western blot: from 1/1000 upwards.
Reactivities Human
Conjugation Unconjugated

Human IgG (Fc specific) mouse monoclonal antibody, clone 4A10, Ascites

Applications ELISA: 1/10000.
Western Blot: 1/500 - 1/2000.
Immunofluorescence: 1/200 - 1/1000.
Reactivities Human
Conjugation Unconjugated

Human IgM (Mu chain specific) mouse monoclonal antibody, clone SA-DA4, Purified

Applications Indirect Immunofluorescent staining of IgM+ human B lymphocytes: ≤ 1 µg/106 cells.
ELISA.
Immunohistochemistry on Frozen Sections.
Western blotting.
Dot- and Slot-Immunoblotting.
Immunocytochemistry.
Reactivities Human
Conjugation Unconjugated