Secondary Antibodies

Human IgM mouse monoclonal antibody, clone DM3A3, Purified

Applications Immunohistochemistry on frozen sections: 1-2 µg/ml (1:200 - 1:400).
Immunohistochemistry on paraffin sections: Proteinase K pretreatment for antigen retrieval is recommended.
Suggested positive control: Human tonsil.
Reactivities Human
Conjugation Unconjugated

Human IgA (secretory) mouse monoclonal antibody, clone B781M, Purified

Applications ELISA: 1/20,000 dilution will yield > 1.0 O.D. using Alkaline Phosphatase conjugated Rabbit anti-Mouse Ig.
Reactivities Human
Conjugation Unconjugated

Human IgM mouse monoclonal antibody, clone 101, Purified

Applications ELISA.
Reactivities Human
Conjugation Unconjugated

Human IgM mouse monoclonal antibody, clone 47.2.E9, Purified

Applications ELISA (1/5000-1/10000)
Reactivities Human
Conjugation Unconjugated

Human IgA (Fc specific) mouse monoclonal antibody, clone 15D6, Purified

Applications ELISA: 1/500-1/1000.
Reactivities Human
Conjugation Unconjugated

Human IgM mouse monoclonal antibody, clone R1/69, Supernatant

Applications Immunohistochemistry on Formalin-Fixed Paraffin Embedded Sections: Use a dilution of 1/25-1/50 in an ABC method (30 minutes at room temperature).
Enzymatic predigestion with proteolytic enzymes is recommended for paraffin embedded tissue sections.
Recommended Positive Control: Tonsil.
Reactivities Human
Conjugation Unconjugated

Human IgA + IgG + IgM (light chain) mouse monoclonal antibody, clone 7A9

Reactivities Human
Conjugation Unconjugated

Human IgG2 (Fc specific) mouse monoclonal antibody, clone 52G1

Reactivities Human
Conjugation Unconjugated

Human IgE (C epsilon 3 specific) (in vitro) mouse monoclonal antibody, clone 4F4cc

Reactivities Human
Conjugation Unconjugated

Human IgE mouse monoclonal antibody, clone IA2, HRP

Applications ELISA: 1/500 - 1/1000; This HRP conjugated antibody may be used as a detection reagent in a sandwich ELISA for IgE, utilising SM1814P as capture reagent.
Reactivities Human
Conjugation HRP

Mouse IgG3 (heavy chain) rat monoclonal antibody, clone LO-MG3-13, Purified

Applications ELISA (5 µg/ml): This antibody may be used as a Coating antibody in ELISA in combination with LO-MG3-7 (SM1022B) as the Detection reagents.
Reactivities Mouse
Conjugation Unconjugated

Mouse IgM (heavy chain) rat monoclonal antibody, clone LO-MM-3, Purified

Applications ELISA: 5 µg/ml (Coating).
This antibody may be used as a Coating antibody in Sandwich ELISA in combination with LO-MM-9 (SM1025B) and Streptavidin-HRP as the detection reagents.
Reactivities Mouse
Conjugation Unconjugated

Mouse IgG2a (heavy chain) rat monoclonal antibody, clone LO-MG2a-9, Purified

Applications ELISA: 5µg/ml; This antibody may be used as a coating antibody in ELISA in combination with LO-MG2a-7 (SM1018HRP) as the detection reagent.
Reactivities Mouse
Conjugation Unconjugated

Mouse IgG2b (heavy chain) rat monoclonal antibody, clone LO-MG2b-1, Purified

Applications ELISA: 5 µg/ml; This antibody may be used as a coating antibody in ELISA in combination with LO-MK1 (SM1496P) as the detection reagent.
Reactivities Mouse
Conjugation Unconjugated

Mouse IgG rat monoclonal antibody, clone cocktail, Purified

Applications ELISA: 1.25 µg/ml.
Reactivities Mouse
Conjugation Unconjugated