Secondary Antibodies

Human IgM (heavy chain) mouse monoclonal antibody, clone IM373, Purified

Applications ELISA: Use Antibody without BSA for Coating.
Flow Cytometry: 0.5-1 μg/106 cells.
Immunofluorescence: 0.5-1 μg/ml.
Western Blot: 0.5-1 μg/ml.
Immunoprecipitation: 0.5-1 μg/500 μg protein lysate.
Immunohistochemistry on Frozen and Formalin-Fixed Sections: 0.5-1 μg/ml for 30 minutes at RT.
Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
Recommended Positive Control
: 293T, Raji or hPBL cells, Tonsil or Spleen.
Reactivities Human
Conjugation Unconjugated

Human IgM (heavy chain) mouse monoclonal antibody, clone IM373, Purified

Applications ELISA: Use Antibody without BSA for Coating.
Flow Cytometry: 0.5-1 μg/106 cells.
Immunofluorescence: 0.5-1 μg/ml.
Western Blot: 0.5-1 μg/ml.
Immunoprecipitation: 0.5-1 μg/500 μg protein lysate.
Immunohistochemistry on Frozen and Formalin-Fixed Sections: 0.5-1 μg/ml for 30 minutes at RT.
Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
Recommended Positive Control
: 293T, Raji or hPBL cells, Tonsil or Spleen.
Reactivities Human
Conjugation Unconjugated

Human IgM (heavy chain) mouse monoclonal antibody, clone IM260, Purified

Applications ELISA: Use Antibody without BSA for Coating.
Flow Cytometry: 0.5-1 μg/106 cells.
Immunofluorescence: 0.5-1 μg/ml.
Western Blot: 0.5-1 μg/ml.
Immunoprecipitation: 0.5-1 μg/500 μg protein lysate.
Immunohistochemistry on Frozen and Formalin-Fixed Paraffin Sections: 0.5-1 μg/ml for 30 minutes at RT.
Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
Positive Control: 293T, Raji or hPBL cells, Tonsil or Spleen.
Reactivities Human
Conjugation Unconjugated

Human IgM (heavy chain) mouse monoclonal antibody, clone IM260, Purified

Applications ELISA: Use Antibody without BSA for Coating.
Flow Cytometry: 0.5-1 μg/106 cells.
Immunofluorescence: 0.5-1 μg/ml.
Western Blot: 0.5-1 μg/ml.
Immunoprecipitation: 0.5-1 μg/500 μg protein lysate.
Immunohistochemistry on Frozen and Formalin-Fixed Paraffin Sections: 0.5-1 μg/ml for 30 minutes at RT.
Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
Positive Control: 293T, Raji or hPBL cells, Tonsil or Spleen.
Reactivities Human
Conjugation Unconjugated

Human IgE mouse monoclonal antibody, clone 4H10, Purified

Applications Flow Cytometry: 4 µg/ml.
ELISA.
Western Blot.
Reactivities Human
Conjugation Unconjugated

Human IgG (Fc heavy chain specific) mouse monoclonal antibody, clone EM-07, FITC

Applications Direct Immunofluorescence analysis: 2-5 µg/ml.
Reactivities Human
Conjugation FITC

Human IgG (Fc specific) mouse monoclonal antibody, clone 21G/3D3, Purified

Applications ELISA.
Reactivities Human
Conjugation Unconjugated

Human IgG mouse monoclonal antibody, clone B573M, Purified

Applications ELISA.
Lateral Flow Assays.
Reactivities Human
Conjugation Unconjugated

Human IgE mouse monoclonal antibody, clone 4H10, FITC

Applications Flow cytometry: 2-5 µg/ml.
Reactivities Human
Conjugation FITC

Kappa light chain, mouse anti human, clone κ -117 Biotin

Applications Tested for immunohistochemistry (IHC) Approximate working dilution for IHC: Frozen sections: 0.25µg/ml (1:800) Paraffin sections: Has been described to work with proteinase K pretreatment. Optimal dilutions should be determined by the end user. Suggested positive control: Human tonsil.
Reactivities Human
Conjugation Biotin

Lambda light chain, mouse anti human, clone λ-1 Biotin

Applications Tested for immunohistochemistry (IHC) Approximate working dilution for IHC: Frozen sections: 0.125µg/ml (1:1600) Paraffin sections: Has been described to work with proteinase K pretreatment. Optimal dilutions should be determined by the end user. Suggested positive control: Human tonsil.
Reactivities Human
Conjugation Biotin

Human IgM (Mu chain, Fc-Region) (in vitro) mouse monoclonal antibody, clone 2B9

Reactivities Human
Conjugation Unconjugated

Human IgG3 (Hinge Region) mouse monoclonal antibody, clone HP6050, AP

Applications ELISA: 1/500-1/1,000.
Western blotting.
Dot- and Slot-Immunoblotting
Immunohistochemistry on Frozen Sections.
Reactivities Human
Conjugation AP

Human IgG3 (Hinge Region) mouse monoclonal antibody, clone HP6050, Biotin

Applications ELISA: 1/5,000-1/10,000.
Western blotting.
Dot- and Slot-Immunoblotting.
Immunocytochemistry.
Immunohistochemistry on Frozen Sections.
Reactivities Human
Conjugation Biotin

Human IgE (Epsilon chain specific) mouse monoclonal antibody, clone HP6029, Purified

Applications ELISA: Purified (UNLB) antibody ≤1 ug/mL.
Western blotting
Dot- and slot-immunoblotting.
Immunocytochemistry.
Immunohistochemistry on Ffrozen Sections.
Reactivities Human
Conjugation Unconjugated