Secondary Antibodies

Rabbit IgG F(ab')2 specific goat polyclonal antibody, HRP

Applications Suitable for Immunoblotting (Western or Dot blot), ELISA, Immunoperoxidase electron microscopy and Immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency.
Recommended Dilutions:
ELISA: 1/10,000-1/50,000.
Western Blot: 1/1,000-1/10,000.
Immunohistochemistry: 1/500-1/2,500.
Reactivities Rabbit
Conjugation HRP

Rabbit IgG (H+L chain, adsorbed) goat polyclonal antibody, HRP

Applications Suitable for Immunoblotting (Western or dot blot), ELISA, Immunoperoxidase electron microscopy and Immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency.
Recommended Dilutions:
ELISA: 1/10,000-1/150,000.
Western blot: 1/1,500-1/5,000.
Immunohistochemistry: 1/750-1/2,500.
Reactivities Rabbit
Conjugation HRP

Rabbit IgG (Fc specific, adsorbed) goat polyclonal antibody, HRP

Applications Suitable for Immunoblotting (Western or Dot blot), ELISA, Immunoperoxidase electron microscopy and Immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency.
Recommended Dilutions:
ELISA: 1:20,000-1:80,000.
Western Blot: 1:2,000-1:10,000.
Immunohistochemistry: 1:500-1:2,500.
Reactivities Rabbit
Conjugation HRP

Rabbit IgG (H+L chain) chicken polyclonal antibody, HRP

Applications Suitable for Immunoblotting (Western or Dot blot), ELISA, Immunoperoxidase electron microscopy and Immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency.
Recommended Dilutions:
ELISA: 1/140,000.
Western blot: 1/2,000-1/10,000.
Immunohistochemistry: 1/1,000-1/5,000.
Reactivities Rabbit
Conjugation HRP

Mouse IgG (H+L chain) chicken polyclonal antibody, HRP

Applications Suitable for Immunoblotting (Western or Dot blot), ELISA, Immunoperoxidase electron microscopy and Immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency.
Recommended Dilutions:
ELISA: 20,000-1/100,000.
Western Blot: 1/2,000-1/10,000.
Immunohistochemistry: 1/500-1/2,500.
Reactivities Mouse
Conjugation HRP

Human IgA (alpha chain) rabbit polyclonal antibody, HRP

Applications Suitable for Immunoblotting (Western or Dot blot), ELISA, Immunoperoxidase electron microscopy and Immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency.
Recommended Dilutions:
ELISA: 1/56,000
Western Blot: 1/1,000-1/5,000
Immunohistochemistry: 1/500-1/2,500.
Reactivities Human
Conjugation HRP

Chicken IgG / Chicken IgY (F(ab)2 specific) rabbit polyclonal antibody, HRP

Applications Suitable for Immunoblotting (Western or Dot blot), ELISA, Immunoperoxidase electron microscopy and Immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency.
Recommended dilutions: This product has been assayed against 1.0 µg of Chicken IgG in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:5,000 to 1:20,000 of the reconstitution concentration is suggested for this product.
Reactivities Chicken
Conjugation HRP

Chicken IgG / Chicken IgY (Fc specific) rabbit polyclonal antibody, HRP

Applications Suitable for Immunoblotting (Western or Dot blot), ELISA, Immunoperoxidase electron microscopy and Immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency.
Recommended Dilutions: This product has been assayed against 1.0 µg of Chicken IgG in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:6,000 to 1:37,000 of the reconstitution concentration is suggested for this product.
Reactivities Chicken
Conjugation HRP

Goat IgG (H+L chain), adsorbed donkey polyclonal antibody, HRP

Applications Suitable for Immunoblotting (Western or Dot blot), ELISA, Immunoperoxidase electron microscopy and Immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency.
Recommended Dilutions:
ELISA: 1/130,000.
Western blot: 1/2,000-1/10,000.
Immunohistochemistry: 1/500-1/2,500.
Reactivities Goat
Conjugation HRP

Mouse IgG3 (gamma 3 chain specific) rabbit polyclonal antibody, HRP

Applications Suitable for Immunoblotting (Western or dot blot: 1/1,000-1/5,000), ELISA (1/31,000), Immunoperoxidase Electron Microscopy and Immunohistochemistry (1/500-1/2,500) as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency.
Reactivities Mouse
Conjugation HRP

Mouse IgG (F(ab)2 specific), adsorbed rabbit polyclonal antibody, HRP

Applications Suitable for Immunoblotting (Western or Dot blot), ELISA, immuno-peroxidase electron microscopy and immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency.
Recommended Dilutions: This product has been assayed against 1.0 ug of Mouse IgG in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:8,000 to 1:38,000 of the reconstitution concentration is suggested for this product.
Reactivities Mouse
Conjugation HRP

Mouse IgG (gamma chain specific) rabbit polyclonal antibody, HRP

Applications Suitable for Immunoblotting (Western or Dot blot), ELISA, Immunoperoxidase electron microscopy and Immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency.
Recommended Dilutions:
ELISA: 1/50,000.
Western Blot: 1/1,000-1/5,000.
Immunohistochemistry: 1/500-1/2,500.
Reactivities Mouse
Conjugation HRP

Mouse IgG (F(ab)2 specific) rabbit polyclonal antibody, HRP

Applications Suitable for Immunoblotting (Western or Dot blot), ELISA, Immunoperoxidase electron microscopy and Immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency.
Recommended Dilutions:
This product has been assayed against 1.0 ug of Mouse IgG in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:2,000 to 1:12,000 is suggested for this product.
Reactivities Mouse
Conjugation HRP

Mouse IgG (H+L chain), adsorbed rabbit polyclonal antibody, HRP

Applications Suitable for Immunoblotting (1/500-1/2,500, Western or dot blot), ELISA (1/5,000-1/20,000), Immunoperoxidase electron microscopy and Immunohistochemistry (1/200-1/1,000) as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency.
Reactivities Mouse
Conjugation HRP

Human IgM (Fc5 mu specific) rabbit polyclonal antibody, HRP

Applications Suitable for Immunoblotting (Western or Dot blot), ELISA, Immunoperoxidase electron microscopy and Immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency.
Recommended Dilutions:
ELISA: 1/15,000-1/60,000.
Western Blot: 1/2,000-1/10,000.
Immunohistochemistry: 1/1,000-1/5,000.
Reactivities Human
Conjugation HRP