Secondary Antibodies

Mouse IgG (H+L chain) rabbit polyclonal antibody, Ig Fraction

Applications Immunoprecipitation.
Immunodiffusion.

Ouchterlony Test:
Sample size: 15 µl.
Distance between wells: 5 mm.
Test Time: 5 days.
Temperature: 37°C.
Samples Tested: Normal mouse Serum (NMS), Purified mouse IgG, Normal human serum (NHS), Purified human IgG.
Results:
Specificity: a single band against NMS was detected at dilutions of 1/4 and 1/8 which is homogeneous with band to purified mouse IgG.
No bands were detected against human IgG at 5 mg/ml or NHS at 1/4 dilution.
Protein content: Biuret Assay 14 mg/ml.
Reactivities Mouse
Conjugation Unconjugated

Human IgG rabbit polyclonal antibody, Ig Fraction

Applications Immunoprecipitation.
Immunodiffusion.
Reactivities Human
Conjugation Unconjugated

Rat IgM goat polyclonal antibody, Purified

Applications ELISA 1ug/ml - 10ug/ml, Western Blotting, Flow Cytometry
Reactivities Rat
Conjugation Unconjugated

Rat IgA goat polyclonal antibody, Purified

Applications ELISA: 1µg/ml - 10µg/ml.
Western Blot.
Immunofluorescence.
Reactivities Reptiles, Rat
Conjugation Unconjugated

Mouse IgM goat polyclonal antibody

Applications Immunohistochemistry on frozen and paraffin sections.
ELISA: 1/2000 - 1/4000.
Western Blot.
Reactivities Mouse
Conjugation Unconjugated

Mouse IgG2a goat polyclonal antibody

Applications ELISA: 1/2000 - 1/4000.
Reactivities Mouse
Conjugation Unconjugated

Rat IgG2c rabbit polyclonal antibody, Purified

Applications ELISA: 1µg/ml - 10µg/ml.
Western Blot.
Flow Cytometry.
Reactivities Human, Rat
Conjugation Unconjugated

Rat IgG2b rabbit polyclonal antibody, Purified

Applications ELISA: 1µg/ml - 10µg/ml.
Western Blot.
Flow Cytometry.
Reactivities Rat
Conjugation Unconjugated

Rat IgG1 goat polyclonal antibody, Purified

Applications ELISA: 1-10 µg/ml.
Western Blot.
Flow Cytometry.
Reactivities Rat
Conjugation Unconjugated

Mouse IgG (adsorbed) goat polyclonal antibody, Purified

Applications ELISA: 1µg/ml - 10µg/ml.
Western Blot.
Flow Cytometry.
Reactivities Mouse
Conjugation Unconjugated

Sheep IgM (H+L chain) rabbit polyclonal antibody, Azide Free

Applications ELISA.
Dot blot.
Immunoblotting.
Indirect Immunofluorescence.

Can be used as unlabelled primary or secondary reagent for indirect detection techniques, to prepare conjugates with markers of the user’s own choice, to prepare an insoluble immunoaffinity adsorbent or a solid phase antibody reagent by coupling to an artificial carrier and as catching or detection antibody in non-isotopic methodology and solid phase immunochemistry. When applied in any cytochemical or histochemical procedure or solids phase coupling technique, the optimum concentration of the IgG preparation should always be established by titration.
Recommended Working Dilutions:
Histochemistry Use: 1/50-1/250.
ELISA and comparable non-precipitating antibody-binding assays: 1/500-1/5,000.
Precipitin titre: 1/64 when tested against pooled normal sheep serum in agar-block immunodiffusion titration.
Reactivities Sheep
Conjugation Unconjugated

Sheep IgG (H+L chain) rabbit polyclonal antibody, Azide Free

Applications ELISA.
Dot blot.
Immunoblotting.
Indirect Immunofluorescence.

Can be used as unlabelled primary or secondary reagent for indirect detection techniques, to prepare conjugates with markers of the user’s own choice, to prepare an insoluble immunoaffinity adsorbent or a solid phase antibody reagent by coupling to an artificial carrier and as catching or detection antibody in non-isotopic methodology and solid phase immunochemistry. When applied in any cytochemical or histochemical procedure or solids phase coupling technique, the optimum concentration of the IgG preparation should always be established by titration.
Recommended Working Dilutions:
Histochemistry Use: 1/50-1/250.
ELISA and comparable non-precipitating antibody-binding assays: 1/500-1/5.000.
Precipitin titre: 1/64 when tested against pooled normal sheep serum in agar-block immunodiffusion titration.
Reactivities Sheep
Conjugation Unconjugated

Mouse IgG IgA IgM (H&L) Pre-Adsorbed

Applications WB: 1:2,000 - 1:10,000
IHC: 1:1,000 - 1:5,000
ELISA: 1:50,000-1:90,000
Reactivities Mouse
Conjugation Unconjugated

Human IgG Antibody (Min X Ms)

Applications WB: 1:2,000 - 1:10,000
IHC: 1:1,000 - 1:5,000
ELISA: 1:20,000 - 1:100,000
Conjugation Unconjugated

Human (kappa chain) Antibody

Applications WB: 1:1,000 - 1:10,000
IHC: 1:500 - 1:2,000
ELISA: 1:10,000 - 1:20,000
Conjugation Unconjugated