Human IgA rabbit polyclonal antibody, Purified
| Applications | Immunohistochemistry on Frozen and Paraffin Sections (ABC method: 1/25-1/100). Formalin-Fixed Paraffin Embedded Tissue Sections requires High temperature antigen unmasking with 10mM citrate buffer, pH 6.0 must be performed prior to the immunostaining. Incubation Time: 30 minutes at room temperature. Positive Control: Tonsil. |
| Reactivities | Human |
| Conjugation | Unconjugated |
Monkey IgG (H+L chain) rabbit polyclonal antibody, Azide Free
| Applications | Can be used as unlabelled primary or secondary reagent for indirect detection techniques, to prepare conjugates with markers of the user’s own choice, to prepare an insoluble immunoaffinity adsorbent or a solid phase antibody reagent by coupling to an artificial carrier and as catching or detection antibody in non-isotopic methodology and solid phase immunochemistry. When applied in any cytochemical or histochemical procedure or solids phase coupling technique, the optimum concentration of the IgG preparation should always be established by titration. Recommended working dilutions: Histochemistry: 1/50 and 1/250. ELISA and comparable non-precipitating antibody-binding assays: 1/500 - 1/5000. |
| Reactivities | Monkey |
| Conjugation | Unconjugated |
Monkey IgG (H+L chain) goat polyclonal antibody, Azide Free
| Applications | As unlabelled primary or secondary reagent for indirect detection techniques, to prepare conjugates with markers of the user’s own choice, to prepare an insoluble immunoaffinity adsorbent or a solid phase antibody reagent by coupling to an artificial carrier and as catching or detection antibody in non-isotopic methodology and solid phase immunochemistry. When applied in any cytochemical or histochemical procedure or solids phase coupling technique, the optimum concentration of the IgG preparation should always be established by titration. Recommended working dilutions: In histochemistry are usually between 1/50 and 1/250. In ELISA and comparable non-precipitating antibody-binding assays between 1/500 and 1/5000. |
| Reactivities | Monkey |
| Conjugation | Unconjugated |
Rat IgG2ab (subclass specific) goat polyclonal antibody, Azide Free
| Applications | As unlabelled primary or secondary reagent for indirect detection of IgG2a at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates; to prepare conjugates of the user’s own choice; to prepare an insoluble immunoaffinity adsorbent or a solid phase antibody reagent by coupling to an artificial carrier and as catching antibody in non-isotopic methodology and solid phase immunochemistry. When applied in any cytochemical or histochemical staining procedure or solid phase coupling technique, the optimum concentration of the IgG preparation should be established by titration before being used. Recommended working dilutions: Histochemistry: 1/100 - 1/250. ELISA and comparable non-precipitating antibody-binding assays: 1/500 - 1/5000. |
| Reactivities | Rat |
| Conjugation | Unconjugated |
Rat IgG2b (subclass specific) goat polyclonal antibody, Azide Free
| Applications | Can be used as unlabelled primary or secondary reagent for indirect detection of IgG2b at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates; to prepare conjugates of the user’s own choice; to prepare an insoluble immunoaffinity adsorbent or a solid phase antibody reagent by coupling to an artificial carrier and as catching antibody in non-isotopic methodology and solid phase immunochemistry. When applied in any cytochemical or histochemical staining procedure or solid phase coupling technique, the optimum concentration of the IgG preparation should be established by titration before being used. Recommended working dilutions: Histochemistry: 1/100 - 1/500. ELISA and comparable non-precipitating antibody-binding assays: 1/500 - 1/5000. |
| Reactivities | Rat |
| Conjugation | Unconjugated |
Human IgE (Fc specific) goat polyclonal antibody, Azide Free
| Applications | Can be used as unlabelled secondary antibody for Indirect detection of IgE in Human cell, tissue substrates and body fluids in immunofluorescence and immunoenzyme assay methods; for the production of immunoconjugates with a selected marker; to prepare insoluble immunoaffinity adsorbents by coupling to an artificial carrier; as catching or detecting antibody reagent in non-isotopic assay methodology (e.g. ELISA) to identify and measure IgE in human serum or other body fluid. When applied in any immunocytochemical or histochemical staining procedure or solid phase coupling technique, the optimum concentration of the IgG preparation should be established. Recommended Working Dilutions: Histochemical and Cytochemical Use: 1/100-1/250. ELISA and comparable non-precipitating antibody-binding assays: 1/500-1/5,000. Performance testing: Isotype specificity and quantitative specific recognition ability are further evaluated at the high level of sensitivity by direct singe and simultaneous double staining of different types of human cells, using counterstaining with reference conjugates with a different marker. The immunoconjugate is further tested in ELISA-type assays. |
| Reactivities | Human |
| Conjugation | Unconjugated |
Mouse IgE (Fc specific) goat polyclonal antibody, Azide Free
| Applications | As unlabelled secondary antibody for indirect detection of IgE in mouse cell, tissue substrates and body fluids in immunofluorescence and immunoenzyme assay methods; for the production of immunoconjugates with a selected marker; to prepare insoluble immunoaffinity adsorbents by coupling to an artificial carrier; as catching or detecting antibody reagent in non-isotopic assay methodology (e.g. ELISA) to identify and measure IgE in mouse serum or other body fluid. When applied in any immunocytochemical or histochemical staining procedure or solid phase coupling technique, the optimum concentration of the IgG preparation should be established. Recommended working dilutions: Histochemical and Cytochemical: 1/100 - 1/250. ELISA and comparable non-precipitating antibody-binding assays: 1/500 - 1/5000. Performance testing: Isotype specificity and quantitative specific recognition ability are further evaluated at the high level of sensitivity by direct singe and simultaneous double staining of different types of mouse cells, using counterstaining with reference conjugates with a different marker. The immunoconjugate is further tested in ELISA-type assays. |
| Reactivities | Mouse |
| Conjugation | Unconjugated |
Human IgG (H+L chain) chicken polyclonal antibody, Aff - Purified
| Applications | ELISA. Western Blot (1/1000). Immunocytochemistry. Immunohistochemistry (1/250). Quality Control: Antibodies were tested for specificity using Double-Immunodiffusion assays in agarose. |
| Reactivities | Human |
| Conjugation | Unconjugated |
Rabbit IgG (H+L chain) chicken polyclonal antibody, Aff - Purified
| Applications | ELISA. Western Blot (1/1000). Immunocytochemistry. Immunohistochemistry (1/250). Quality Control: Antibodies were tested for specificity using Double-Immunodiffusion assays in agarose. |
| Reactivities | Rabbit |
| Conjugation | Unconjugated |
Guinea Pig IgG (H&L) Antibody Pre-Adsorbed
| Applications | WB: 1:2,000 - 1:10,000 ELISA: 1:25,000 - 1:75,000 |
| Conjugation | Unconjugated |
Monkey IgG (H&L) Antibody
| Applications | WB: 1:10,000 - 1:60,000 ELISA: 1:50,000 - 1:100,000 |
| Conjugation | Unconjugated |
Mouse IgG3 (subclass specific) goat polyclonal antibody, Azide Free
| Applications | Can be used as unlabelled primary or secondary reagent for indirect detection of IgG3 at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates; to prepare conjugates of the user’s own choice; to prepare an insoluble immunoaffinity adsorbent or a solid phase antibody reagent by coupling to an artificial carrier and as catching antibody in non-isotopic methodology and solid phase immunochemistry. When applied in any cytochemical or histochemical staining procedure or solid phase coupling technique, the optimum concentration of the IgG preparation should be established by titration before being used. Recommended Working Dilutions: Histochemistry: 1/100-1/500. ELISA and comparable non-precipitating antibody-binding assays: 1/500-1/5.000. Performance testing: Titre, specificity and reactivity of the subclass specific cross-adsorbed IgG fraction is further evaluated in a number of highly sensitive non-precipitating antibody-binding assay systems. These performance tests include direct single and double identification of cIg in mouse cell and tissue substrates and their evaluation as second antibody in the analysis of human cells and tissues after reacting with a primary monoclonal mouse antibody to a human antigen. The quantitative specific recognition ability is verified in double staining procedures together with reference reagents of known specificity and reactivity. |
| Reactivities | Mouse |
| Conjugation | Unconjugated |
Rabbit IgA (Fc specific) goat polyclonal antibody, Azide Free
| Applications | As unlabelled primary or secondary reagent for indirect detection techniques, to prepare conjugates with markers of the user’s own choice, to prepare an insoluble immunoaffinity adsorbent or a solid phase antibody reagent by coupling to an artificial carrier and as catching or detection antibody in non-isotopic methodology and solid phase immunochemistry. When applied in any cytochemical or histochemical procedure or solids phase coupling technique, the optimum concentration of the IgG preparation should always be established by titration. Recommended Working Dilutions: Histochemistry: 1/100-1/250. ELISA and comparable non-precipitating antibody-binding assays: 1/500-1/2500. Antibody titre: Precipitin titre not less than 1/32 when tested against pooled normal rabbit serum in agar-block immunodiffusion titration. |
| Reactivities | Rabbit |
| Conjugation | Unconjugated |
Rat IgE (Fc specific) goat polyclonal antibody, Azide Free
| Applications | This antibody can be used: • As unlabelled secondary antibody for indirect detection of IgE in Rat cell, tissue substrates and body fluids. • In Immunofluorescence and immunoenzyme assay methods. • For the production of immunoconjugates with a selected marker. • To prepare insoluble immunoaffinity adsorbents by coupling to an artificial carrier. • As catching or detecting antibody reagent in non-isotopic assay methodology (e.g. ELISA) to identify and measure IgE in rat serum or other body fluid. Recommended Dilutions: Histochemistry and Cytochemistry: 1/100-1/250. ELISA and comparable non-precipitating antibody-binding assays: 1/500-1/5,000. |
| Reactivities | Rat |
| Conjugation | Unconjugated |
Rabbit IgM (Fc specific) goat polyclonal antibody, Azide Free
| Applications | Can be used as unlabelled primary or secondary reagent for indirect detection techniques, to prepare conjugates with markers of the user’s own choice, to prepare an insoluble immunoaffinity adsorbent or a solid phase antibody reagent by coupling to an artificial carrier and as catching or detection antibody in non-isotopic methodology and solid phase immunochemistry. When applied in any cytochemical or histochemical procedure or solids phase coupling technique, the optimum concentration of the IgG preparation should always be established by titration. Recommended working dilutions: Histochemistry: 1/50 - 1/250. ELISA and comparable non-precipitating antibody-binding assays: 1/500 - 1/3000. Antibody titre: Precipitin titre not less than 1/32 when tested against pooled normal rabbit serum in agar-block immunodiffusion titration. |
| Reactivities | Rabbit |
| Conjugation | Unconjugated |
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