Secondary Antibodies

Human IgG (adsorbed F(ab)2 Fragment) goat polyclonal antibody, Biotin

Applications Suitable for Western Blot, ELISA, Immunohistochemistry, Immunomicroscopy as well as other antibody based assays using streptavidin or avidin conjugates requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity. Other applications not tested.
Recommended Dilutions: This product has been assayed against 1.0 µg of Human IgG in a standard capture ELISA using Peroxidase Conjugated Streptavidin and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:20000 to 1:100000 of the reconstitution concentration is suggested for this product.
Reactivities Human
Conjugation Biotin

Hamster IgG (H+L chain, F(ab)2 Fragment) rabbit polyclonal antibody, Texas Red

Applications Suitable for Immunomicroscopy and Flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
Flow Cytometry: 1:2,000-1:10,000.
Immunofluorescence microscopy: 1:500-1:2,500.
Reactivities Hamster
Conjugation Texas Red

Goat IgG (H+L chain), F(ab)2 Fragment, adsorbed rabbit polyclonal antibody, Biotin

Applications Suitable for Immunoblotting, ELISA, Immunohistochemistry, Immunomicroscopy as well as other antibody based assays using streptavidin or avidin conjugates requiring lot-to-lot consistency.
Recommended Dilutions: This product has been assayed against 1.0 ug of Goat IgG in a standard capture ELISA using Peroxidase Conjugated Streptavidin and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature.
A working dilution of 1:40,000 to 1:200,000 is suggested for this product.
The following general recommendations are suggested as starting dilutions for:
Immunoblotting: 1:10,000,
Enzyme Immunohisto-chemistry on tissue sections: 1:500-1:5,000.
Flow cytometry and fluorescence Immunohisto/cytochemistry: 1:200-1:1,000.
Reactivities Goat
Conjugation Biotin

Goat IgG (H+L chain), adsorbed rabbit polyclonal antibody, AP

Applications Suitable for Immunoblotting (Western or Dot blot), ELISA and Immunohistochemistry as well as other phosphatase-antibody based enzymatic assays requiring lot-to-lot consistency.
ELISA: 1:10,000 - 1:40,000.
Western Blot: 1:500 - 1:5,000.
Immunohistochemistry: 1:200 - 1:2,000.
Reactivities Goat
Conjugation AP

Rat IgG (H+L chain), Fab Fragment goat polyclonal antibody, HRP

Applications Suitable for Immunoblotting (Western or Dot blot), ELISA, Immunoperoxidase electron microscopy and Immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency.
ELISA: 1:10,000 - 1:40,000.
Western Blot: 1:1,000 - 1:4,000.
Reactivities Rat
Conjugation HRP

Guinea pig IgG (H+L chain), F(ab)2 Fragment goat polyclonal antibody, AP

Applications Suitable for Immunoblotting (Western or dot blot), ELISA and Immunohistochemistry as well as other phosphatase-antibody based enzymatic assays requiring lot-to-lot consistency. This product has been assayed against 1.0 ug of Guinea Pig IgG in a standard capture ELISA using pNPP p-nitrophenyl phosphate as a substrate for 30 minutes at room temperature. A working dilution of 1:1,000 to 1:5,000 of the reconstitution concentration is suggested for this product.
Reactivities Guinea Pig
Conjugation AP

Guinea pig IgG (H+L chain), F(ab)2 Fragment goat polyclonal antibody, Biotin

Applications Suitable for Immunoblotting, ELISA, Immunohistochemistry, Immunomicroscopy as well as other antibody based assays using streptavidin or avidin conjugates requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.
Recommended Dilutions: This product has been assayed against 1.0 ug of Guinea Pig IgG in a standard capture ELISA using Peroxidase Conjugated Streptavidin and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature.
A working dilution of 1:10,000 to 1:50,000 is suggested for this product.
Reactivities Guinea Pig
Conjugation Biotin

Goat IgG (H+L chain), F(ab)2 Fragment, adsorbed rabbit polyclonal antibody, TRITC

Applications Suitable for Immunomicroscopy and Flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
Reactivities Goat
Conjugation TRITC

Goat IgG (H+L chain), F(ab)2 Fragment, adsorbed rabbit polyclonal antibody, Texas Red

Applications Suitable for Immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
Flow Cytometry: 1:2,000 - 1:10,000.
Immunofluorescence microscopy: 1:500 - 1:2,500.
Reactivities Goat
Conjugation Texas Red

Goat IgG (H+L chain), F(ab)2 Fragment, adsorbed rabbit polyclonal antibody, HRP

Applications Suitable for Immunoblotting (Western or dot blot), ELISA, Immunoperoxidase electron microscopy and Immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency.
Recommended Dilutions: This product has been assayed against 1.0 ug of Goat IgG in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:10,000 to 1:50,000 is suggested for this product.
Reactivities Goat
Conjugation HRP

Goat IgG (H+L chain), F(ab)2 Fragment, adsorbed rabbit polyclonal antibody, Aff - Purified

Applications Suitable for Immunoprecipitation, Immunodiffusion, conjugation and most immunological methods requiring lot-to-lot consistency, high titer and specificity.
ELISA: 1:5,000 - 1:20,000.
Reactivities Goat
Conjugation Unconjugated

Mouse IgG (H+L chain), Fab Fragment goat polyclonal antibody, HRP

Applications Suitable for Immunoblotting (Western or Dot blot), ELISA, Immunoperoxidase electron microscopy and Immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency.
Recommended Dilutions
ELISA: 1/10,000-1:100,000.
Westrn Blot: 1/2,000-1/20,000.
Immunohistochemistry: 1:500-1:2,500.
Reactivities Mouse
Conjugation HRP

Human IgG (H+L chain), Fab Fragment goat polyclonal antibody, Texas Red

Applications Suitable for Immunomicroscopy and Flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.
Reactivities Human
Conjugation Texas Red

Mouse IgG (H+L chain), Fab Fragment goat polyclonal antibody, Biotin

Applications Suitable for Immunoblotting, ELISA,
Immunohistochemistry, Immunomicroscopy as well as other antibody based assays using streptavidin or avidin conjugates requiring lot-to-lot consistency.
Recommended dilutions:
This product has been assayed against 1.0 ug of Mouse IgG in a standard capture ELISA using Peroxidase Conjugated Streptavidin and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:30,000 to 1:140,000 of the reconstitution concentration is suggested for this product.
Reactivities Mouse
Conjugation Biotin

Rat IgG (H+L chain), Fab Fragment goat polyclonal antibody, Biotin

Applications Immunoblotting
ELISA
Immunohistochemistry
Immunomicroscopy
as well as other antibody based assays using streptavidin or avidin conjugates requiring lot-to-lot consistency.
Recommended Dilutions: This product has been assayed against 1.0 ug of Rat IgG in a standard capture ELISA using Peroxidase Conjugated Streptavidin and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:20,000 to 1:110,000 of the reconstitution concentration is suggested for this product.
Reactivities Rat
Conjugation Biotin