Secondary Antibodies

Rat IgG (H+L chain), F(ab)2 Fragment goat polyclonal antibody, PE

Applications Suitable for Immunomicroscopy and Flow Cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.
The maximum amount of reagent required to stain 1 x 10e6 cells in Flow Cytometry is approximately 1.0 µg of antibody conjugate. Lesser amounts of reagent may be sufficient for staining. As a general guideline dilutions of 1:100 to 1:250 should be suitable for most applications.
Reactivities Rat
Conjugation PE

Rabbit IgG (F(ab)2 specific adsorbed F(ab)2 Fragment) goat polyclonal antibody, TRITC

Applications Suitable for Immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.
Reactivities Rabbit
Conjugation TRITC

Human IgG (H+L chain), adsorbed rabbit polyclonal antibody, AP

Applications Suitable for immunoblotting (western or dot blot), ELISA and immunohistochemistry as well as other phosphatase-antibody based enzymatic assays requiring lot-to-lot consistency.
Recommended Dilutions:
ELISA: 1:2,000-1:40,000.
Western Blot: 1: 500-1:2,000.
Immunohistochemistry: 1:200-1:1,000.
Reactivities Human
Conjugation AP

Human IgG (H+L chain), adsorbed rabbit polyclonal antibody, Biotin

Applications Suitable for Immunoblotting, ELISA, Immunohistochemistry, Immunomicroscopy as well as other antibody based assays using streptavidin or avidin conjugates requiring lot-to-lot consistency.
Recommended Dilutions: This product has been assayed against 1.0 ug of Human IgG in a standard capture ELISA using Peroxidase Conjugated and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:17,000 to 1:75,000 of the reconstitution concentration is suggested for this product.
Reactivities Human
Conjugation Biotin

Human IgG (H+L chain), adsorbed rabbit polyclonal antibody, Texas Red

Applications Suitable for Immunomicroscopy and Flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
Reactivities Human
Conjugation Texas Red

Human IgG (H+L chain), adsorbed rabbit polyclonal antibody, TRITC

Applications Suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
Reactivities Human
Conjugation TRITC

Human IgG (H+L chain), adsorbed rabbit polyclonal antibody, FITC

Applications Suitable for Immunomicroscopy and Flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
Reactivities Human
Conjugation FITC

Equine IgG (F(ab)2 specific) rabbit polyclonal antibody, AP

Applications Suitable for Immunoblotting (Western blot: 1/500-1/2,000 or Dot blot), ELISA (1/2,000-1/8,000) and Immunohistochemistry (1/200-1/1,000) as well as other phosphatase-antibody based enzymatic assays requiring lot-to-lot consistency.
Reactivities Horse
Conjugation AP

Human IgG (H+L chain) chicken polyclonal antibody, HRP

Applications Suitable for Immunoblotting (1/1,000-1/5,000, Western or Dot blot), ELISA (1/10,000-1/40,000), Immunoperoxidase electron microscopy and Immunohistochemistry (1/500-1/2,500) as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency.
Reactivities Human
Conjugation HRP

Human IgG (H+L chain) chicken polyclonal antibody, AP

Applications ELISA: 1/2,000 - 1/10,000.
Western Blot: 1/500 - 1/2,500.
Immunohistochemistry: 1/200 - 1/1,000.
Reactivities Human
Conjugation AP

Equine IgG (Fc specific) rabbit polyclonal antibody, AP

Applications Suitable for immunoblotting (western or dot blot), ELISA and immunohistochemistry as well as other phosphatase-antibody based enzymatic assays requiring lot-to-lot consistency.
Recommended Dilutions: This product has been assayed against 1.0 ug of Horse IgG in a standard capture ELISA using pNPP (p-nitrophenyl phosphate) as a substrate for 30 minutes at room temperature. A working dilution of 1:500 to 1:2,000 of the reconstitution concentration is suggested for this product.
Reactivities Horse
Conjugation AP

Hamster IgG (H+L chain), adsorbed goat polyclonal antibody, Texas Red

Applications Suitable for Immunomicroscopy and Flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
Reactivities Hamster
Conjugation Texas Red

Hamster IgG (H+L chain), adsorbed goat polyclonal antibody, TRITC

Applications Suitable for Immunomicroscopy and Flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
Reactivities Hamster
Conjugation TRITC

Hamster IgG (H+L chain), adsorbed goat polyclonal antibody, FITC

Applications Suitable for Immunomicroscopy and Flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
Reactivities Hamster
Conjugation FITC

Golden Syrian Hamster IgG (F(ab)2 specific) rabbit polyclonal antibody, AP

Applications Suitable for immunoblotting (western or dot blot), ELISA and immunohistochemistry as well as other phosphatase-antibody based enzymatic assays requiring lot-to-lot consistency.
This product has been assayed against 1.0 ug of Hamster IgG in a standard capture ELISA using pNPP p-nitrophenyl phosphate as a substrate for 30 minutes at room temperature.
A working dilution of 1:1,000 to 1:5,000 of the reconstitution concentration is suggested for this product. Optimal dilutions of this antibody are dependent on conditions and should be determined by the user.
Reactivities Golden Syrian Hamster
Conjugation AP