Secondary Antibodies

Sheep IgM (Fc specific) rabbit polyclonal antibody, Azide Free

Applications ELISA.
Dot blot.
Immunoblotting.
Indirect Immunofluorescence.

Can be used as unlabelled primary or secondary reagent for indirect detection of IgM at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates; to prepare conjugates of the user’s own choice; to prepare an insoluble immunoaffinity adsorbent or a solid phase antibody reagent by coupling to an artificial carrier and as catching antibody in nonisotopic methodology and solid phase immunochemistry. When applied in any cytochemical or histochemical staining procedure or solid phase coupling technique, the optimum concentration of the IgG preparation should be established by titration
before being used.
Recommended Working Dilutions:
Histochemistry Use: 1/50-1/250.
ELISA and comparable non-precipitating antibody-binding assays: 1/500-1/5,000.
Precipitin titre: 1/64 when tested against pooled normal sheep serum in agar-block immunodiffusion titration.
Reactivities Sheep
Conjugation Unconjugated

Sheep IgG (Fc specific) rabbit polyclonal antibody, FITC

Applications ELISA.
Immunocytochemistry.
(In)direct Immunofluorescence.

Immunohistochemistry on Frozen Sections.
Can be used In direct staining of cytoplasmic IgG in fixed sheep cells and tissue substrates; to identify circulating IgG antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen or immune complex using a reference antibody of sheep in the middle layer of the test procedure. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Recommended Working Dilutions: 1/20-1/80.
Reactivities Sheep
Conjugation FITC

Sheep IgG F(c) specific(adsorbed) rabbit polyclonal antibody, Aff - Purified

Applications Suitable for Immunoprecipitation, Immunodiffusion, conjugation and most immunological methods requiring lot-to-lot consistency, high titer and specificity..
Reactivities Sheep
Conjugation Unconjugated

Sheep IgA rabbit polyclonal antibody, HRP

Applications ELISA: 1:10,000 - 1:100,000.
Reactivities Sheep
Conjugation HRP

Sheep IgG (H+L chain) rabbit polyclonal antibody, HRP

Applications ELISA: 1/400,000.
Western Blot: 1/1,000-1/5,000.
Immunohistochemistry: 1/500-1/2,500.
Reactivities Sheep
Conjugation HRP

Sheep IgG (H+L chain) rabbit polyclonal antibody, AP

Applications Suitable for Immunoblotting (Western or dot blot), ELISA and Immunohistochemistry as well as other phosphatase-antibody based enzymatic assays requiring lot-to-lot consistency.
Recommended Dilutions:
ELISA: 1/25,000.
Western Blot: 1/500-1/2,000.
Immunohistochemistry: 1/200-1/1,000.
Reactivities Sheep
Conjugation AP

Sheep IgG F(ab)2 specific rabbit polyclonal antibody, Aff - Purified

Applications Suitable for Immunoprecipitation, Immunodiffusion, conjugation and most immunological methods requiring lot-to-lot consistency, high titer and specificity.
Reactivities Sheep
Conjugation Unconjugated

Sheep IgM rabbit polyclonal antibody, Purified

Applications ELISA: 1:100 - 1:10,000.
Reactivities Sheep
Conjugation Unconjugated

Sheep IgM rabbit polyclonal antibody, FITC

Applications Flow Cytometry: 1:200 - 1:2000.
Reactivities Sheep
Conjugation FITC

Sheep IgM rabbit polyclonal antibody, HRP

Applications ELISA: 1/10,000-1/100,000.
Reactivities Sheep
Conjugation HRP

Sheep IgA rabbit polyclonal antibody, Purified

Applications ELISA: 1:100 - 1:10,000.
Reactivities Sheep
Conjugation Unconjugated

Sheep IgG F(ab)2 specific rabbit polyclonal antibody, TRITC

Applications Suitable for Immunomicroscopy and Flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
Reactivities Sheep
Conjugation TRITC

Sheep IgG (H+L chain), adsorbed rabbit polyclonal antibody, TRITC

Applications Suitable for Immunomicroscopy and Flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
Reactivities Sheep
Conjugation TRITC

Sheep IgG (H+L chain), adsorbed rabbit polyclonal antibody, Aff - Purified

Applications Suitable for Immunoprecipitation, Immunodiffusion, conjugation and most immunological methods requiring lot-to-lot consistency, high titer and specificity.
Reactivities Sheep
Conjugation Unconjugated

Sheep IgG (H+L chain), adsorbed rabbit polyclonal antibody, FITC

Applications Suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
Reactivities Sheep
Conjugation FITC