Mouse IgG1 goat polyclonal antibody, FITC
| Applications | Flow Cytometry: Use 10 µl of neat-1/10 diluted antibody to label 106 cells in 100 µl. Immunohistochemistry on Frozen Sections. Immunocytochemistry: neat-1/10. |
| Reactivities | Mouse |
| Conjugation | FITC |
Mouse IgG (Fc specific) goat polyclonal antibody, AP
| Applications | Suitable for Immunoprecipitation, Immunodiffusion, conjugation and most immunological methods requiring lot-to-lot consistency, high titer and specificity. Recommended Dilutions: ELISA: 1/2,000-1/100,000 Western blot: 1/500-1/2,500 Immunohistochemistry: 1/200-1/1,000. Note: This product has been assayed against 1.0 µg of Mouse IgG in a standard capture ELISA using pNPP p-nitrophenyl phosphate code #NPP-10 as a substrate for 30 minutes at room temperature. A working dilution of 1/500 to 1/5,000 of the reconstitution concentration is suggested for this product. |
| Reactivities | Mouse |
| Conjugation | AP |
Mouse IgG2b Antibody Rhodamine Conjugated Pre-adsorbed
| Applications | WB: 1:2,000 - 1:10,000 IHC: 1:1,000 - 1:5,000 IF: 1:1,000 - 1:5,000 ELISA: 1:2,000 - 1:10,000 FLISA: 1:20,000 - 1:100,000 |
| Reactivities | Mouse |
| Conjugation | TRITC |
Mouse IgG1 Antibody Fluorescein Conjugated Pre-adsorbed
| Applications | WB: 1:2,000 - 1:10,000 IHC: 1:1,000 - 1:5,000 IF: 1:1,000 - 1:5,000 FC: 1:200 - 1:500 FLISA: 1:10,000 - 1:50,000 |
| Reactivities | Mouse |
| Conjugation | FITC |
Mouse IgG2a Antibody Fluorescein Conjugated Pre-adsorbed
| Applications | WB: 1:2,000 - 1:10,000 IHC: 1:1,000 - 1:5,000 IF: 1:1,000 - 1:5,000 FC: 1:200 - 1:500 FLISA: 1:10,000 - 1:50,000 |
| Reactivities | Mouse |
| Conjugation | FITC |
Mouse IgG2b Antibody ATTO 550 Conjugated Pre-adsorbed
| Applications | WB: >1:10,000 IHC: >1:5,000 IF: >1:5,000 FC: 1:500 - 1:2,500 FLISA: >1:20,000 |
| Reactivities | Mouse |
| Conjugation | ATTO 550 |
Mouse IgG1 (Gamma 1 chain) ATTO 550 Conjugated Antibody
| Applications | WB: >1:10,000 IHC: >1:5,000 IF: >1:5,000 FC: 1:500 - 1:2,500 FLISA: >1:20,000 |
| Reactivities | Mouse |
| Conjugation | ATTO 550 |
Mouse IgG2a Antibody ATTO 532 Conjugated Pre-adsorbed
| Applications | WB: >1:10,000 IHC: >1:5,000 IF: >1:5,000 FC: 1:500 - 1:2,500 FLISA: >1:20,000 |
| Reactivities | Mouse |
| Conjugation | ATTO 532 |
Mouse IgG2a Antibody ATTO 550 Conjugated Pre-adsorbed
| Applications | WB: >1:10,000 IHC: >1:5,000 IF: >1:5,000 FC: 1:500 - 1:2,500 FLISA: >1:20,000 |
| Reactivities | Mouse |
| Conjugation | ATTO 550 |
Mouse IgG3 Antibody ATTO 550 Conjugated Pre-adsorbed
| Applications | WB: >1:10,000 IHC: >1:5,000 IF: >1:5,000 FC: 1:500 - 1:2,500 FLISA: >1:20,000 |
| Reactivities | Mouse |
| Conjugation | ATTO 550 |
Mouse IgG (H&L) Secondary Antibody Fluorescein Conjugated Pre-Adsorbed
| Applications | WB: User Optimized IHC: User Optimized IF: 1:1,000 - 1:5,000 FC: 1:500 - 1:2,500 FLISA: 1:10,000 - 1:50,000 |
| Reactivities | Mouse |
| Conjugation | FITC |
Mouse IgG (H+L chain), adsorbed donkey polyclonal antibody, HRP
| Applications | Suitable for Immunoblotting (1/2,000-1/20,000 for Western blot), ELISA (1/180,000), Immunoperoxidase electron microscopy and Immunohistochemistry (1/1,000-1/5,000) as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency. Recommended Dilutions: This product has been assayed against 1.0 µg of Mouse IgG in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1/20,000 to 1/80,000 of the reconstitution concentration is suggested for this product. |
| Reactivities | Mouse |
| Conjugation | HRP |
Mouse IgM (Fc specific) rabbit polyclonal antibody, HRP
| Applications | ELISA. Dot blot. Immunoblotting. Immunocytochemistry. Immunohistochemistry on Paraffin Sections. In enzyme-immunocytochemical and immunohistochemical staining for the detection of IgM at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates; to demonstrate circulating IgM antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen using a reference antibody of mouse origin known to be of the IgM isotype in the middle layer of the indirect test procedure; in non-isotopic assay methodology (e.g. ELISA) to measure IgM in mouse serum or other body fluids. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Recommended Working Dilutions Histochemical and cytochemical Use: 1/100- 1/500. ELISA and comparable non-precipitating antibody-binding assays: 1/1,000- 1/5,000. |
| Reactivities | Mouse |
| Conjugation | HRP |
Mouse IgM (Fc specific) rabbit polyclonal antibody, Biotin
| Applications | In immunocytochemical and immunohistochemical staining of IgM at the cellular and subcellular level of appropriately treated cell and tissue substrates; to demonstrate circulating IgM antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen using a reference antibody of mouse origin known to be of the IgM isotype in the middle layer of the indirect test procedure; in non-isotopic assay methodology (e.g. ELISA) to measure IgM in mouse serum or other body fluids. As a second step an avidin or streptavidin conjugate of the user’s choice has to be used. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Recommended Working Dilutions: Histochemical and cytochemical Use: 1/100- 1/2500. ELISA and comparable non-precipitating antibody-binding assays: 1/1000- 1/5000. |
| Reactivities | Mouse |
| Conjugation | Biotin |
Mouse IgA+IgG+IgM (H+L chain) goat polyclonal antibody, Biotin
| Applications | ELISA: 1/5000-1/20000. |
| Reactivities | Mouse |
| Conjugation | Biotin |
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