Secondary Antibodies

Mouse IgM (Mu chain specific) rat monoclonal antibody, clone 1B4B1, AP

Applications ELISA: 1/1000-1/2000.
Also used as a second step reagent for mouse IgM monoclonal antibodies.
Reactivities Mouse
Conjugation AP

Mouse IgG2a (gamma 2a chain specific) rat monoclonal antibody, clone SB84a, AP

Applications ELISA: 1/500-1/1000.
Also used as a second step reagent for mouse IgG2a monoclonal antibodies.
Reactivities Mouse
Conjugation AP

Mouse IgE goat polyclonal antibody, AP

Applications ELISA: 1:100 - 1:1000.
Reactivities Mouse
Conjugation AP

Mouse IgG (H+L chain), adsorbed donkey polyclonal antibody, AP

Applications Suitable for Immunoblotting (western or dot blot), ELISA and immunohistochemistry as well as other phosphatase-antibody based enzymatic assays requiring lot-to-lot consistency.
Recommended Dilutions:
This product has been assayed against 1.0 µg of Mouse IgG in a standard capture ELISA usingp-nitrophenyl phosphate (pNPP) as a substrate for 30 minutes at room temperature.
A working dilution of 1:500 to 1:2,000 of the reconstitution concentration is suggested for this product.
Reactivities Mouse
Conjugation AP

Mouse IgG (H+L chain) chicken polyclonal antibody, AP

Applications Suitable for Immunoblotting (Western or Dot blot), ELISA and Immunohistochemistry as well as other phosphatase-antibody based enzymatic assays requiring lot-to-lot consistency.
Recommended Dilutions:
This product has been assayed against 1.0 ug of Mouse IgG in a standard capture ELISA using pNPP (p-nitrophenyl phosphate) as a substrate for 30 minutes at room temperature.
A working dilution of 1:1,000 to 1:5,000 of the stated concentration is suggested for this product.
Reactivities Mouse
Conjugation AP

Mouse IgG (Fc specific) rabbit polyclonal antibody, AP

Applications Suitable for Immunoblotting (Western or Dot blot), ELISA and Immunohistochemistry as well as other phosphatase-antibody based enzymatic assays requiring lot-to-lot consistency.
Recommended Dilutions:
This product has been assayed against 1.0 ug of Mouse IgG in a standard capture ELISA using pNPP p-nitrophenyl phosphate as a substrate for 30 minutes at room temperature.
A working dilution of 1:500 to 1:2,500 of the reconstitution concentration is suggested for this product.
Reactivities Mouse
Conjugation AP

Mouse IgG (F(ab)2 specific) rabbit polyclonal antibody, AP

Applications Suitable for Immunoblotting (Western or Dot blot), ELISA and Immunohistochemistry as well as other phosphatase-antibody based enzymatic assays requiring lot-to-lot consistency.
Recommended Dilutions:
This product has been assayed against 1.0 µg of Mouse IgG in a standard capture ELISA using pNPP p-nitrophenyl phosphate as a substrate for 30 minutes at room temperature.
A working dilution of 1:1,000 to 1:4,700 of the reconstitution concentration is suggested for this product.
Reactivities Mouse
Conjugation AP

Mouse IgG (H+L chain), adsorbed rabbit polyclonal antibody, AP

Applications Suitable for Immunoblotting (1/100-1/1,000; Western or dot blot), ELISA (1/1,000-1/4000) and Immunohistochemistry (1/100-1/500) as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency.
Reactivities Mouse
Conjugation AP

Mouse IgA (H+L chain) goat polyclonal antibody, AP

Applications Suitable for Immunoblotting (Western or Dot blot), ELISA and Immunohistochemistry as well as other phosphatase-antibody based enzymatic assays requiring lot-to-lot consistency.
Recommended Dilutions:
ELISA: 1/2,000-1/10,000.
Western blot: 1/500-1/2,500.
Immunohistochemistry: 1/200-1/1,000.
Reactivities Mouse
Conjugation AP

Mouse IgA (H+L chain), F(ab)2 Fragment, adsorbed goat polyclonal antibody, AP

Applications Suitable for Immunoblotting (Western or dot blot), ELISA and Immunohistochemistry as well as other phosphatase-antibody based enzymatic assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.
Recommended Dilutions:
ELISA: 1:6,300
Western Blot: 1:200 - 1:1,000
Immunohistochemistry: 1:100 - 1:500
Reactivities Mouse
Conjugation AP

Mouse lambda light chain rabbit polyclonal antibody, AP

Applications Suitable for Immunoblotting (Western or dot blot), ELISA and Immunohistochemistry as well as other phosphatase-antibody based enzymatic assays requiring lot-to-lot consistency.
This product has been assayed against 1.0 ug of Mouse IgG in a standard capture ELISA using pNPP p-nitrophenyl phosphate as a substrate for 30 minutes at room temperature. A working dilution of 1:1,000 to 1:2,000 of the reconstitution concentration is suggested for this product.
Reactivities Mouse
Conjugation AP

Mouse IgG (F(ab)2 specific), adsorbed rabbit polyclonal antibody, AP

Applications Suitable for Immunoblotting (Western or Dot blot), ELISA and Immunohistochemistry as well as other phosphatase-antibody based enzymatic assays requiring lot-to-lot consistency.
Recommended Dilutions: This product has been assayed against 1.0 µg of Mouse IgG in a standard capture ELISA using pNPP p-nitrophenyl phosphate as a substrate for 30 minutes at room temperature. A working dilution of 1:500 to 1:3,500 of the reconstitution concentration is suggested for this product.
Reactivities Mouse
Conjugation AP

Mouse IgM (Mu chain specific) rabbit polyclonal antibody, AP

Applications Immunoblotting (Western or dot blot).
Immunohistochemistry.
ELISA (1:6,000) and other phosphatase-antibody based enzymatic assays requiring lot-to-lot consistency.
Reactivities Mouse
Conjugation AP

Mouse IgG (gamma chain specific) rabbit polyclonal antibody, AP

Applications Suitable for Immunoblotting (Western or Dot blot), ELISA and Immunohistochemistry as well as other phosphatase-antibody based enzymatic assays requiring lot-to-lot consistency.
Recommended Dilutions:
This product has been assayed against 1.0 ug of Mouse IgG in a standard capture ELISA using p-nitrophenyl phosphate (pNPP) as a substrate for 30 minutes at room temperature.
A working dilution of 1:1,000 to 1:2,000 of the reconstitution concentration is suggested for this product.
Reactivities Mouse
Conjugation AP

Mouse IgG2b (gamma 2b chain specific) rabbit polyclonal antibody, AP

Applications Suitable for Immunoblotting (Western or Dot blot), ELISA and immunohistochemistry as well as other phosphatase-antibody based enzymatic assays requiring lot-to-lot consistency.
Recommended Dilutions:
ELISA: 1/8,000-1/32,000.
Western Blot: 1/1,000-1/3,000.
Immunohistochemistry: 1/200-1/1,000.
Reactivities Mouse
Conjugation AP