Secondary Antibodies

Human IgA mouse monoclonal antibody, clone NI 69 (A89-034) and NI 184 (A89-035), Biotin

Applications To identify the presence of IgA in Human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as ELISA, immunoperoxidase staining of cytoplasmic IgA, and immunoblotting. As a second step an avidin or streptavidin conjugate of the customer’s choice have to be used.
General Recommended Dilutions:
Histochemical Use: 1/50-1/200
ELISA: from 1/250 upwards.
Western blot: from 1/500 upwards.
Reactivities Human
Conjugation Biotin

Human IgA2 (A2m2 Allotype Specific) mouse monoclonal antibody, clone NI 194-3 (A89-040), Biotin

Applications To identify the presence of IgA2(m)2 in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as ELISA, Immunoperoxidase staining of cytoplasmic IgA2(m)2, and Immunoblotting. As a second step an avidin or streptavidin conjugate of the customer’s choice have to be used.
General Recommended Dilutions:
Histochemical Use: 1/50-1/200
ELISA: from 1/200 upwards.
Western blot: from 1/200 upwards.
Reactivities Human
Conjugation Biotin

Human IgA1 mouse monoclonal antibody, clone NI 69-11 (A89-036), Biotin

Applications To identify the presence of IgA1 in Human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as Radioimmunoassay, ELISA, indirect immunoperoxidase and Immunofluorescence staining of cytoplasmic IgA1, Haemagglutination and Immunoblotting.
General Recommended Dilutions:
Histochemical Use: 1/50-1/200.
ELISA: from 1/250 upwards.
Western blot: 1/500 upwards.
Reactivities Human
Conjugation Biotin

Human IgG (Fd region) mouse monoclonal antibody, clone HP6045, Biotin

Applications ELISA (both capture and detection).
Western Blot.
Radioimmunoassay
Reactivities Human
Conjugation Biotin

Human IgA (alpha chain) goat polyclonal antibody, Biotin

Applications Immunoblotting, ELISA, immunohistochemistry, immunomicroscopy as well as other antibody
based assays using streptavidin or avidin conjugates:
ELISA: 1/60,000-1/600,000
Western Blot: 1/2,000-1/10,000
Immunohistochemistry: 1/1,000-1/5,000
Reactivities Human
Conjugation Biotin

Human IgA + IgG + IgM goat polyclonal antibody, Biotin

Applications Western Blot: 1/5000-1/20000.
ELISA: 1/400000.
Immunohistochemistry on frozen and paraffin sections: 1/1000-1/5000.
Reactivities Human
Conjugation Biotin

Human IgG (gamma chain specific) mouse monoclonal antibody, clone JDC-10, Biotin

Applications Indirect Immunofluorescent staining of IgG+ human B lymphocytes: < / = 1 µg/10e6 cells.
ELISA: 1/5,000-1/10,000.
Flow Cytometry: 1 µg / 10e6 cells
Western Blotting
Reactivities Human
Conjugation Biotin

Human IgM (Mu chain specific) mouse monoclonal antibody, clone UHB, Biotin

Applications Indirect Immunofluorescent staining of IgM+ human B lymphocytes: ≤ 1 µg/10e6 cells.
ELISA: 1/1,000-1/2,000.
Reactivities Human
Conjugation Biotin

Human IgE goat polyclonal antibody, Biotin

Applications ELISA: 1/10,000-1/100,000.
Western Blot: 1/10,000-1/100,000.
Reactivities Human, Monkey
Conjugation Biotin

Human IgG4 (pFC specific) mouse monoclonal antibody, clone NI 315 (HP 6206), Biotin

Applications To identify the presence of IgG4 in Human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as ELISA, immunoperoxidase staining of cytoplasmic IgG4, and Immunoblotting. As a second step an avidin or streptavidin conjugate of the customer’s choice have to be used.
General Recommended Dilutions:
Histochemical Use: 1/50-1/250.
ELISA: from 1/100 upwards.
Western blot: from 1/500 upwards.
Reactivities Human
Conjugation Biotin

Human IgG (gamma chain specific) rabbit polyclonal antibody, Biotin

Applications Suitable for Immunoblotting, ELISA, Immunohistochemistry, immunomicroscopy as well as other antibody based assays using streptavidin or avidin conjugates requiring lot-to-lot consistency.
Recommended Dilutions: This product has been assayed against 1.0 ug of Human IgG in a standard capture ELISA using Peroxidase Conjugated Streptavidin and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature.
A working dilution of 1:5,000 to 1:35,000 of the reconstitution concentration is suggested for this product.
Reactivities Human
Conjugation Biotin

Canine IgE mouse monoclonal antibody, clone E6-71A1, Biotin

Applications ELISA: The antibody may be used as a detection antibody in ELISA techniques in combination with SM1498P as capture antibody.
Western Blotting: Under reducing conditions, this antibody detects a band of approximately 62-65 kDa in samples of affinity purified Dog IgE.
Reactivities Canine, Human
Conjugation Biotin

Human IgG2 (Fc specific) mouse monoclonal antibody, clone 31-7-4, Biotin

Applications ELISA: 1/5,000-1/20,000.
Immunofluorescence < / = 1 µg/10e6 cells.
Reactivities Human
Conjugation Biotin

Human IgD (Delta chain specific) mouse monoclonal antibody, clone IADB6, Biotin

Applications Indirect Immunofluorescent staining of IgD+ human B lymphocytes: ≤ 1 µg/106 cells.
ELISA: 1/5,000-1/10,000.
Reactivities Human
Conjugation Biotin