Secondary Antibodies

Mouse IgE (heavy chain) rat monoclonal antibody, clone LO-ME-3, Biotin

Applications Useful as second antibody in Immunoassays, such as ELISA's (good binding on plastic) and Immunohistochemistry on Frozen Sections.
Reactivities Mouse
Conjugation Biotin

Mouse IgA (heavy chain) rat monoclonal antibody, clone LO-MA-7, Biotin, Purified

Applications Immunoassay.
ELISA: good binding on plastic.
Immunohistochemistry on frozen sections.
Reactivities Mouse
Conjugation Biotin

Mouse IgD (Delta heavy chain) rat monoclonal antibody, clone 11-26, Biotin

Applications Flow Cytometry: < / = 1µg/10e6 cells. (See Ref.1-5)
Reactivities Mouse
Conjugation Biotin

Mouse IgG (H+L chain), adsorbed rabbit polyclonal antibody, Biotin

Applications Suitable for Immunoblotting, ELISA, Immunohistochemistry, Immunomicroscopy as well as other antibody based assays using streptavidin or avidin conjugates requiring lot-to-lot consistency.
Recommended Dilutions:
This product has been assayed against 1.0 ug of Mouse IgG in a standard capture ELISA using Peroxidase Conjugated Streptavidin and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:20,000 to 1:80,000 of the reconstitution concentration is suggested for this product.
Reactivities Mouse
Conjugation Biotin

Mouse IgE (heavy chain) rat monoclonal antibody, clone LO-ME-3, Biotin

Applications ELISA (Use at 0.5 µg/ml as a detection antibody, avidity 3 x 10e9 M-1).
Reactivities Mouse
Conjugation Biotin

Mouse IgM (heavy chain) rat monoclonal antibody, clone LO-MM-9, Biotin

Applications ELISA: 250ng/ml.
Reactivities Mouse
Conjugation Biotin

Mouse IgG2a (heavy chain) rat monoclonal antibody, clone LO-MG2a-7, Biotin

Applications ELISA: 0.5µg/ml - 1.0µg/ml.
Reactivities Mouse
Conjugation Biotin

Mouse IgG1 (heavy chain) rat monoclonal antibody, clone LO-MG1-2, Biotin

Applications ELISA: 500ng/ml.
Reactivities Mouse
Conjugation Biotin

Mouse IgG + IgM (H+L chain), adsorbed goat polyclonal antibody, Biotin

Applications Suitable for Immunoblotting (Western Blot, 1:2,000-1:10,000), ELISA (1:20,000-1:100,000), Immunohistochemistry, Immunomicroscopy as well as other antibody based assays using streptavidin or avidin conjugates requiring lot-to-lot consistency.
Reactivities Mouse
Conjugation Biotin

Mouse IgG2b (gamma 2b chain specific) rabbit polyclonal antibody, Biotin

Applications Suitable for Immunoblotting, ELISA, Immunohistochemistry, Immunomicroscopy as well as other antibody based assays using streptavidin or avidin conjugates requiring lot-to-lot consistency.
Recommended Dilutions: This product has been assayed against 1.0 µg of Mouse IgG2b in a standard capture ELISA using Peroxidase Conjugated Streptavidin and ABTS (2,2'-azino-bis-[3-ethylbenthiozoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:15,000 to 1:75,000 is suggested for this product.
Reactivities Mouse
Conjugation Biotin

Mouse IgG3 (gamma 3 chain specific) rabbit polyclonal antibody, Biotin

Applications Suitable for Immunoblotting, ELISA, Immunohistochemistry, Immunomicroscopy as well as other antibody based assays using streptavidin or avidin conjugates requiring lot-to-lot consistency.
Recommended Dilutions:
ELISA: 1/8,000-1/32,000.
Western Blot: 1/1,000-1/5,000.
Immunohistochemistry: 1/500- 1/2,000.
Reactivities Mouse
Conjugation Biotin

Mouse IgG (F(ab)2 specific) rabbit polyclonal antibody, Biotin

Applications Immunoblotting, ELISA, Immunohistochemistry, Immunomicroscopy as well as other antibody based assays using streptavidin or avidin conjugates requiring lot-to-lot consistency.
Recommended Dilutions:
This product has been assayed against 1.0 µg of Mouse IgG in a standard capture ELISA using Peroxidase Conjugated Streptavidin and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:20,000 to 1:100,000 of the reconstitution concentration is suggested for this product.
Reactivities Mouse
Conjugation Biotin

Mouse IgG (gamma chain specific) rabbit polyclonal antibody, Biotin

Applications Suitable for Immunoblotting, ELISA, Immunohistochemistry, Immunomicroscopy as well as other antibody based assays using streptavidin or avidin conjugates requiring lot-to-lot consistency.
Recommended Dilutions:
This product has been assayed against 1.0 µg of Mouse IgG in a standard capture ELISA using Peroxidase Conjugated Streptavidin and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature.
A working dilution of 1:10,000 to 1:50,000 of the reconstitution concentration is suggested for this product.
Reactivities Mouse
Conjugation Biotin

Mouse IgG (H+L chain) chicken polyclonal antibody, Biotin

Applications Suitable for Immunoblotting, ELISA, Immunohistochemistry, Immunomicroscopy as well as other antibody based assays using streptavidin or avidin conjugates requiring lot-to-lot consistency.
Recommended Dilutions:
This product has been assayed against 1.0 ug of Mouse IgG in a standard capture ELISA using Peroxidase Conjugated Streptavidin and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:4,000 to 1:20,000 of the reconstitution concentration is suggested for this product.
Reactivities Mouse
Conjugation Biotin

Mouse IgG1 (gamma 1 chain specific) rabbit polyclonal antibody, Biotin

Applications Suitable for Immunoblotting (Western blot: 1/1,000-1/5,000), ELISA (1/25,000), Immunohistochemistry (1/500-1/3,000) and immunomicroscopy as well as other antibody based assays using streptavidin or avidin conjugates and requiring lot-to-lot consistency.
Reactivities Mouse
Conjugation Biotin