Secondary Antibodies

Rat IgG1 (gamma 1 chain specific) mouse monoclonal antibody, clone G1 1C5, Biotin

Applications Flow Cytometry: < / = 0.3 µg/10e6 cells.
Immunofluorescence Microscopy: Identification and enumeration of IgG1+ cells.
ELISA: Can be used as Capture antibody.
Reactivities Rat
Conjugation Biotin

Rat IgG1 (gamma 1 chain specific) mouse monoclonal antibody, clone G1 7E7, Biotin

Applications Flow Cytometry: ≤ 1 µg/106 cells.
Immunofluorescence Microscopy: Identification and enumeration of IgG1+ cells.
ELISA: 1/5,000-1/20,000.
Reactivities Rat
Conjugation Biotin

Mouse IgA (alpha chain specific) rat monoclonal antibody, clone 11-44-2, Biotin

Applications Flow Cytometry: < / = 1 µg/10e6 cells.
Identification and enumeration of IgA+.
Immunofluorescence Microscopy: Identification and enumeration of IgA+.
ELISA.
Reactivities Mouse
Conjugation Biotin

Goat IgG (H+L chain) rabbit polyclonal antibody, Biotin

Applications Suitable for Immunoblotting, ELISA, Immunohistochemistry, Immunomicroscopy as well as other assays using streptavidin/avidin conjugates requiring lot-to-lot consistency.
Recommended Dilutions:
ELISA: 1/20,000-1/1,00,000.
Western Blot: 1/2,000-1/10,000.
Immunohistochemistry: 1/1,000-1/5,000.
Reactivities Goat
Conjugation Biotin

Mouse IgG (H+L chain) rabbit polyclonal antibody, Biotin

Applications Immunoblotting, ELISA, Immunohistochemistry, Immunomicroscopy as well as other antibody based assays using streptavidin or avidin conjugates requiring lot-to-lot consistency.
Recommended Dilutions:
ELISA: 1/300,000.
Western blot: 1/2,000-1/10,000.
Immunohistochemistry: 1/1,000-1/5,000.
Reactivities Mouse
Conjugation Biotin

Biotin Conjugated Rabbit Monoclonal Anti-Human Ig Light Chains antibody 3, clone RM129

Applications ELISA: 0.02ug/mL – 0.25ug/mL; IHC, ICC: 0.5ug/mL – 2ug/mL
Reactivities Human Ig light chain
Conjugation Biotin

Rat IgG (H+L chain, adsorbed) goat polyclonal antibody, Biotin

Applications

ELISA: 1/5000-1/20000. 
Western Blot. 
Immunofluorescence. 
Immunohistochemistry on Frozen Sections. 
Immunohistochemistry on Paraffin Sections.

Reactivities Rat
Conjugation Biotin

Human IgA (alpha chain) goat polyclonal antibody, Biotin

Applications Immunoblotting, ELISA, immunohistochemistry, immunomicroscopy as well as other antibody
based assays using streptavidin or avidin conjugates:
ELISA: 1/60,000-1/600,000
Western Blot: 1/2,000-1/10,000
Immunohistochemistry: 1/1,000-1/5,000
Reactivities Human
Conjugation Biotin

Mouse IgM (Mu chain specific) rat monoclonal antibody, clone 1B4B1, Biotin

Applications Flow Cytometry: < / = 1 µg/10e6 cells.
Identification and enumeration of IgE+ cells.
Immunofluorescence Microscopy: Identification and enumeration of IgE+ cells .
ELISA.
Also used as a second step reagent for mouse IgM monoclonal antibodies.
Reactivities Mouse
Conjugation Biotin

Mouse IgG2a (gamma 2a chain specific) rat monoclonal antibody, clone SB84a, Biotin

Applications Flow Cytometry: Identification and enumeration of IgG2a+.
Immunofluorescence Microscopy: Identification and enumeration of IgG2a+.
ELISA.
Also used as a second step reagent for mouse IgG2a monoclonal antibodies.
Reactivities Mouse
Conjugation Biotin

Mouse IgE (Epsilon heavy chain) rat monoclonal antibody, clone 23G3, Biotin

Applications Flow Cytometry: < / = 1 µg/10e6 cells.
Identification and enumeration of IgE+ cells.
Immunofluorescence Microscopy: Identification and enumeration of IgE+ cells .
ELISA: 1/5000-1/10000.
Enzyme-Linked-Immunosorbent-Assay.
Reactivities Mouse
Conjugation Biotin

Fab Golden Syrian Hamster IgG (H&L) Antibody Biotin Conjugated

Applications WB: 1:2,000 - 1:10,000
IHC: 1:1,000 - 1:5,000
ELISA: 1:200,000
Conjugation Biotin

F(ab')2 Swine IgG (H&L) Antibody Biotin Conjugated

Applications WB: 1:2,000 - 1:20,000
IHC: 1:1,000 - 1:5,000
ELISA: 1:600,000
Conjugation Biotin