Secondary Antibodies

Monkey IgA (Secretory component) goat polyclonal antibody, FITC

Applications Tested in immunoelectrophoresis, double radial immunodiffusion and ELISA against a panel of appropriate secretions and purified Ig isotypes.
Can be used as reagent for the direct detection of secretory component in monkey cells, tissues and body fluids in immunofluorescence; as detection reagent in non-isotopic methodology and solid phase immunochemistry (e.g. ELISA).
This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Working dilutions are usually between 1/20 and 1/80.
Reactivities Chimpanzee, Monkey
Conjugation FITC

Monkey IgA + IgG + IgM (H+L chain) rabbit polyclonal antibody, FITC

Applications Can be used for direct immunofluorescence staining of cytoplasmic Ig of appropriately treated cell and tissue substrates; to demonstrate immunoglobulins or specific antibodies in cells and tissues; to identify circulating antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen or immune complex using a reference antibody of monkey origin in the middle layer of the indirect test procedure. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Working dilutions are usually between 1:20 and 1:80.
Reactivities Monkey
Conjugation FITC

Monkey IgA + IgG + IgM (Fc specific) goat polyclonal antibody, FITC

Applications Can be used for direct immunofluorescence staining of cytoplasmic Ig of appropriately treated cell and tissue substrates; to demonstrate immunoglobulins or specific antibodies in cells and tissues; to identify circulating antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen or immune complex using a reference antibody of monkey origin in the middle layer of the indirect test procedure. The absence of activity to the common Ig/Fab subunit prevents the reaction of this conjugate with immunoglobulins bounds to Fc receptors on non-lymphoid cells. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Working dilutions are usually between 1/20 and 1/120.
Reactivities Monkey
Conjugation FITC

Mouse IgG (H+L chain) sheep polyclonal antibody, FITC

Applications Suitable for Immunomicroscopy and Flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
Reactivities Mouse
Conjugation FITC

Human IgA (Fc specific) mouse monoclonal antibody, clone 15D6, FITC, Purified

Applications ELISA: 1/1000-1/10000.
Immunofluorescence.
Reactivities Human
Conjugation FITC

Human IgG (Fc specific) rabbit polyclonal antibody, FITC

Applications This Fluorescein Conjugated antibody is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
Recommended Dilutions:
FLISA: 1/10,000-1/50,000.
IF MIscroscopy: 1/1000-1/5000.
Reactivities Human
Conjugation FITC

Mouse IgD (Delta heavy chain) rat monoclonal antibody, clone 11-26, FITC

Applications Flow Cytometry: < / = 1 µg/10e6 cells. (See Ref.1-5)
Immunofluorescence.
Reactivities Mouse
Conjugation FITC

Rat IgG2b (Gamma-2b chain specific) mouse monoclonal antibody, clone 2B 10A8, FITC

Applications Flow Cytometry: < / = 0.3 µg/10e6 cells.
Immunofluorescence Microscopy: Identification and enumeration of IgG2b+ cells.
Reactivities Rat
Conjugation FITC

Rat IgG2c (gamma 2c chain specific) mouse monoclonal antibody, clone 2C 8F1, FITC

Applications Flow Cytometry: < / = 0.3 µg/10e6 cells.
Immunofluorescence Microscopy: Identification and enumeration of IgG2c+ cells.
Reactivities Rat
Conjugation FITC

Rat IgG1 (gamma 1 chain specific) mouse monoclonal antibody, clone G1 7E7, FITC

Applications Flow Cytometry: < / = 0.3 µg/10e6 cells.
Immunofluorescence Microscopy: Identification and enumeration of IgG1+ cells.
Reactivities Rat
Conjugation FITC

Mouse IgA (alpha chain specific) rat monoclonal antibody, clone 11-44-2, FITC

Applications FLISA: 1/200-1/400.
Flow Cytometry:
≤ 1 µg/106 cells.
Identification and enumeration of IgA+ cells.
Immunofluorescence Microscopy: Identification and enumeration of IgA+ cells.
Reactivities Mouse
Conjugation FITC

Rat IgG2ab (subclass specific) goat polyclonal antibody, FITC

Applications Can be used to identify and measure IgG2, antigen or antibody, at the cellular and subcellular level by immunofluorescence staining of appropriately treated cell and tissue substrates, and to demonstrate circulating antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen or immune complex using a reference antibody of rat origin in the middle layer of the indirect test procedure. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Working dilutions: 1/20 - 1/80.
Reactivities Rat
Conjugation FITC

Rat IgG2b (subclass specific) goat polyclonal antibody, FITC

Applications Can be used to identify and measure IgG2b, antigen or antibody, at the cellular and subcellular level by immunofluorescence staining of appropriately treated cell and tissue substrates, and to demonstrate circulating antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen or immune complex using a reference antibody of rat origin in the middle layer of the indirect test procedure.
This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Recommended working dilutions: 1/20 - 1/80.
Reactivities Rat
Conjugation FITC

Rabbit IgA+IgG+IgM (H+L chain) goat polyclonal antibody, FITC

Applications Can be used for direct immunofluorescence staining of cytoplasmic Ig of appropriately treated cell and tissue substrates; to demonstrate immunoglobulins or specific antibodies in cells and tissues; to identify circulating antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen or immune complex using a reference antibody of rabbit origin in the middle layer of the indirect test procedure. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Recommended working dilutions: 1/20 - 1/80.
Reactivities Rabbit
Conjugation FITC

Rat IgE (Fc specific) goat polyclonal antibody, FITC

Applications This antibody can be used
In Immunocytochemical and Immunohistochemical staining for the detection of IgE at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates.
To identify and measure IgE in Rat serum or other body fluids.
Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Recommended Dilutions: 1/20-1/80.
Reactivities Rat
Conjugation FITC