Secondary Antibodies

Mouse IgG3 (heavy chain) rat monoclonal antibody, clone LO-MG3-7, FITC, Purified

Applications

Useful as second antibody in Immunoassays, such as ELISA's (good binding on plastic) and Immunohistochemistry on Frozen Sections.
The antibody can be used at 5 μg/ml.

Reactivities Mouse
Conjugation FITC

Mouse IgG2a (gamma 2a chain specific) rabbit polyclonal antibody, FITC

Applications This product is designed for Immunofluorescence Microscopy, Fluorescence based plate assays (FLISA) and Fluorescent Western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
Recommended Dilutions:
FLISA: 1/10,000-1/50,000.
Immunofluorescence: 1/1,000-1/5,000.
Flow Cytometry: 1/500-1/2,500.
Reactivities Mouse
Conjugation FITC

Mouse IgG (Fc specific), F(ab)2 Fragment, adsorbed goat polyclonal antibody, FITC

Applications
This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
Recommended Dilutions:
FLISA: 1/10,000-1/50,000.
IF Microscopy: 1/1,000-1/5,000.
Flow Cytometry: 1/500-1/2,500.

Reactivities Mouse
Conjugation FITC

Mouse lambda light chain goat polyclonal antibody, FITC

Applications Flow cytometry.
Immunofluorescence.
Reactivities Mouse
Conjugation FITC

Mouse IgE (heavy chain) rat monoclonal antibody, clone LO-ME-3, FITC

Applications Flow Cytometry: Use 50µl of 5µg/ml diluted antibody to label 10e6 cells in 100µl.
Reactivities Mouse
Conjugation FITC

Mouse IgE (Epsilon heavy chain) rat monoclonal antibody, clone 23G3, FITC

Applications Flow Cytometry: < / = 1 µg/10e6 cells.
Identification and enumeration of IgE+ cells.
Immunofluorescence Microscopy: Identification and enumeration of IgE+ cells.
Reactivities Mouse
Conjugation FITC

Mouse IgM (Mu chain specific) rat monoclonal antibody, clone 1B4B1, FITC

Applications Flow Cytometry: < / = 1 µg/10e6 cells.
Identification and enumeration of IgM+ cells.
Immunofluorescence Microscopy: Identification and enumeration of IgM+ cells.
Also used as a second step reagent for mouse IgM monoclonal antibodies.
Reactivities Mouse
Conjugation FITC

Mouse IgG (H+L chain) chicken polyclonal antibody, FITC

Applications Immunocytochemistry.
Immunohistochemistry (1/250).
Quality Control: Antibodies were tested for specificity using Double-Immunodiffusion assays in agarose and for non-specific binding using Cryostat sections of unfixed embryonic chick tissues.
Reactivities Mouse
Conjugation FITC

Mouse IgG2c goat polyclonal antibody, FITC

Applications Flow Cytometry.
Reactivities Mouse
Conjugation FITC

Mouse IgG IgA IgM (H&L) Antibody Fluorescein Conjugated Pre-Adsorbed

Applications IF: 1:1,000 - 1:5,000
FC: 1:500 - 1:2,500
FLISA: 1:10,000 - 1:50,000
Reactivities Mouse
Conjugation FITC

Mouse IgG goat polyclonal antibody, FITC, Aff - Purified

Applications Immunofluorescence: 1/500-1/1000. 
Immunohistochemistry.
Reactivities Mouse
Conjugation FITC

Mouse IgG (H+L chain), F(ab)2 Fragment goat polyclonal antibody, FITC

Applications Suitable for Immunomicroscopy and Flow Cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.
Reactivities Mouse
Conjugation FITC

Mouse IgG (H+L chain), F(ab)2 Fragment rabbit polyclonal antibody, FITC

Applications Flow Cytometry: Use 50 µl of 1/25-1/100 diluted antibody to label 106 cells in 100 µl.
Reactivities Mouse
Conjugation FITC

Mouse IgE (heavy chain) rat monoclonal antibody, clone LO-ME-3, FITC

Applications Useful as second antibody in Immunoassays, such as ELISA's
Reactivities Mouse
Conjugation FITC

Mouse IgG (F(ab)2 specific), adsorbed rabbit polyclonal antibody, FITC

Applications Suitable for Immunomicroscopy and Flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
Reactivities Mouse
Conjugation FITC