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Mouse IgM (mu chain) Antibody Peroxidase Conjugated
| Applications | WB: 1:1,000 - 1:5,000 IHC: 1:500 - 1:2,500 ELISA: 1:180,000 |
| Reactivities | Mouse |
| Conjugation | HRP |
Secondary Antibodies
Mouse IgG3 (subclass specific) goat polyclonal antibody, HRP
| Applications | This antibody can be used in Enzyme-Immunocytochemical and Immunohistochemical staining for the detection of IgG3 at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates To demonstrate circulating IgG3 antibodies in serodiagnostic microbiology and autoimmune diseases. To identify a specific antigen using a reference antibody of mouse origin known to be of the IgG3 isotype in the middle layer of the indirect test procedure In non-isotopic assay methodology (e.g. ELISA) to measure IgG3 in Mouse serum or other body fluids. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Working Dilutions: Histochemical/Cytochemical Use: 1/100-1/500. ELISA and comparable non-precipitating antibody-binding assays: 1/1,000-1/7,000 depending on the method used. Working dilutions schould be stored at +4°C, not refrozen, and preferably used the same day. |
| Reactivities | Mouse |
| Conjugation | HRP |
Mouse IgE (Fc specific) rabbit polyclonal antibody, HRP
| Applications | Can be used in enzyme-immunocytochemical and immunohistochemical staining for the detection of IgE at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates; to demonstrate circulating IgE antibodies in mouse serum or other body fluids; in non-isotopic assay methodology (e.g. ELISA) to identify and measure IgE in mouse serum or other body fluids. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Recommended working dilutions: Histochemical and Cytochemical: 1/100 - 1/500. ELISA and comparable non-precipitating antibody-binding assays: 1/500 - 1/5000. |
| Reactivities | Mouse |
| Conjugation | HRP |
Mouse IgM (Fc specific) goat polyclonal antibody, HRP
| Applications | Can be used in enzyme-immunocytochemical and immunohistochemical staining for the detection of IgM at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates; to demonstrate circulating IgM antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen using a reference antibody of mouse origin known to be of the IgM isotype in the middle layer of the indirect test procedure; in non-isotopic assay methodology (e.g. ELISA) to measure IgM in mouse serum or other body fluids. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Working dilutions: For histochemical and cytochemical use are usually between 1/100 and 1/400. In ELISA and comparable non-precipitating antibody-binding assays between 1/1000 and 1/8000 depending on the method used. |
| Reactivities | Mouse |
| Conjugation | HRP |
Mouse IgM (Fc specific) rabbit polyclonal antibody, HRP
| Applications | ELISA. Dot blot. Immunoblotting. Immunocytochemistry. Immunohistochemistry on Paraffin Sections. In enzyme-immunocytochemical and immunohistochemical staining for the detection of IgM at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates; to demonstrate circulating IgM antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen using a reference antibody of mouse origin known to be of the IgM isotype in the middle layer of the indirect test procedure; in non-isotopic assay methodology (e.g. ELISA) to measure IgM in mouse serum or other body fluids. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Recommended Working Dilutions Histochemical and cytochemical Use: 1/100- 1/500. ELISA and comparable non-precipitating antibody-binding assays: 1/1,000- 1/5,000. |
| Reactivities | Mouse |
| Conjugation | HRP |
Mouse lambda light chain rabbit polyclonal antibody, HRP
| Applications | ELISA: 1/10,000 - 1/50,000 . Western Blot: 1/1,000 - 1/5,000. Immunohistochemistry on paraffin sections: 1/500 - 1/2,500. |
| Reactivities | Mouse |
| Conjugation | HRP |
Mouse IgG1 (heavy chain) rat monoclonal antibody, clone LO-MG1-2, HRP
| Applications | ELISA: 500 ng/ml. This product may be used in a direct ELISA or as a detection reagent in a sandwich ELISA together with SM1491P as the capture reagent. |
| Reactivities | Mouse |
| Conjugation | HRP |
Goat Anti-Mouse IgG (H+L) HRP conjugated secondary antibody
| Applications | EIA and Western blots: 1:5,000-1:100,000. Immunohistochemistry: 1:500-1:5,000. Optimal working dilutions must be determined by end user. |
| Reactivities | Mouse |
| Conjugation | HRP |
Goat Anti-Mouse IgG (H+L) HRP conjugated secondary antibody
| Applications | EIA and Western blots: 1:5,000-1:100,000. Immunohistochemistry: 1:500-1:5,000. Optimal working dilutions must be determined by end user. |
| Reactivities | Mouse |
| Conjugation | HRP |
Mouse IgA (alpha chain specific) goat polyclonal antibody, HRP
| Applications | ELISA: 1/4,000-1/8,000. Immunoblotting. Immunohistochemistry. |
| Reactivities | Mouse |
| Conjugation | HRP |
Mouse IgG2a (gamma 2a chain specific) rabbit polyclonal antibody, HRP
| Applications | This secondary antibody anti-Mouse is ideal for investigators who routinely perform titration assays, Western-blot, Immunoprecipitation and more generally immunoassays. Anti-Mouse IgG2a heavy chain antibody is suitable for highly specific immunological methods requiring extremely low background levels, lot-to-lot consistency, high titer and specificity. Recommended Dilutions: ELISA: 1/90,000 Western blot: 1/2,000-1/10,000. Immunohistochemistry: 1/500-1/2,500. |
| Reactivities | Mouse |
| Conjugation | HRP |
Mouse IgG (H+L chain), adsorbed goat polyclonal antibody, HRP
| Applications | ELISA: 1/500-1/1000. Immunohistochemistry on Frozen Sections: 1/50. Immunohistochemistry on Paraffin Sections. |
| Reactivities | Mouse |
| Conjugation | HRP |
Mouse IgG2a (heavy chain) rat monoclonal antibody, clone LO-MG2a-9, HRP
| Applications | ELISA (250 ng/ml). |
| Reactivities | Mouse |
| Conjugation | HRP |
Mouse IgG2a goat polyclonal antibody, HRP
| Applications | ELISA: 1/4000-1/8000. Western Blotting. Immunohistochemistry on Frozen Sections. Immunohistochemistry on Paraffin Sections. |
| Reactivities | Mouse |
| Conjugation | HRP |
Mouse IgG2b goat polyclonal antibody, HRP
| Applications | ELISA: 1/4000-1/8000. Western Blot. Immunohistochemistry on Frozen Sections Immunohistochemistry on Paraffin Sections. |
| Reactivities | Mouse |
| Conjugation | HRP |
