Secondary Antibodies

Mouse IgM (Mu chain specific) rat monoclonal antibody, clone 1B4B1, HRP

Applications ELISA: 1/2000-1/4000.
Also used as a second step reagent for mouse IgM monoclonal antibodies.
Reactivities Mouse
Conjugation HRP

Mouse IgM (Mu chain specific) rat monoclonal antibody, clone SB73a, HRP

Applications ELISA: 1/2000-1/4000.
Also used as a second step reagent for mouse IgM monoclonal antibodies.
Reactivities Mouse
Conjugation HRP

Mouse IgG2a (gamma 2a chain specific) rat monoclonal antibody, clone SB84a, HRP

Applications ELISA: 1/1000-1/2000.
Also used as a second step reagent for mouse IgG2a monoclonal antibodies.
Reactivities Mouse
Conjugation HRP

Mouse IgE goat polyclonal antibody, HRP

Applications ELISA: 1:100 - 1:1000.
Reactivities Mouse
Conjugation HRP

Mouse IgG2c goat polyclonal antibody, HRP

Applications ELISA: 1/10000-1/100000.
Reactivities Mouse
Conjugation HRP

Mouse IgG + IgM (H+L chain), adsorbed goat polyclonal antibody, HRP

Applications Suitable for Immunoblotting (Western or dot blot), ELISA, Immunoperoxidase Electron Microscopy and Immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency.
Recommended Dilutions:
ELISA: 1/40,000.
Western Blot: 1/2,000-1/10,000.
Immunohistochemistry: 1/500-1/2,500.
Reactivities Mouse
Conjugation HRP

Mouse IgG (H+L chain) chicken polyclonal antibody, HRP

Applications Suitable for Immunoblotting (Western or Dot blot), ELISA, Immunoperoxidase electron microscopy and Immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency.
Recommended Dilutions:
ELISA: 20,000-1/100,000.
Western Blot: 1/2,000-1/10,000.
Immunohistochemistry: 1/500-1/2,500.
Reactivities Mouse
Conjugation HRP

Mouse IgG3 (gamma 3 chain specific) rabbit polyclonal antibody, HRP

Applications Suitable for Immunoblotting (Western or dot blot: 1/1,000-1/5,000), ELISA (1/31,000), Immunoperoxidase Electron Microscopy and Immunohistochemistry (1/500-1/2,500) as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency.
Reactivities Mouse
Conjugation HRP

Mouse IgG (F(ab)2 specific), adsorbed rabbit polyclonal antibody, HRP

Applications Suitable for Immunoblotting (Western or Dot blot), ELISA, immuno-peroxidase electron microscopy and immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency.
Recommended Dilutions: This product has been assayed against 1.0 ug of Mouse IgG in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:8,000 to 1:38,000 of the reconstitution concentration is suggested for this product.
Reactivities Mouse
Conjugation HRP

Mouse IgG (gamma chain specific) rabbit polyclonal antibody, HRP

Applications Suitable for Immunoblotting (Western or Dot blot), ELISA, Immunoperoxidase electron microscopy and Immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency.
Recommended Dilutions:
ELISA: 1/50,000.
Western Blot: 1/1,000-1/5,000.
Immunohistochemistry: 1/500-1/2,500.
Reactivities Mouse
Conjugation HRP

Mouse IgG (F(ab)2 specific) rabbit polyclonal antibody, HRP

Applications Suitable for Immunoblotting (Western or Dot blot), ELISA, Immunoperoxidase electron microscopy and Immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency.
Recommended Dilutions:
This product has been assayed against 1.0 ug of Mouse IgG in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:2,000 to 1:12,000 is suggested for this product.
Reactivities Mouse
Conjugation HRP

Mouse IgG (H+L chain), adsorbed rabbit polyclonal antibody, HRP

Applications Suitable for Immunoblotting (1/500-1/2,500, Western or dot blot), ELISA (1/5,000-1/20,000), Immunoperoxidase electron microscopy and Immunohistochemistry (1/200-1/1,000) as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency.
Reactivities Mouse
Conjugation HRP

Mouse IgG (H+L chain), F(ab)2 Fragment, adsorbed goat polyclonal antibody, HRP

Applications Suitable for Immunoblotting (Western or Dot blot), ELISA, Immunoperoxidase electron microscopy and Immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.
Recommended dilution
ELISA: 1:60,000.
Reactivities Mouse
Conjugation HRP