Secondary Antibodies

Human IgG3 (Hinge Region) mouse monoclonal antibody, clone HP6050, PE

Applications FLISA: 1 µg/ml.
Immunocytochemistry.
Immunohistochemistry on Frozen Sections.
Reactivities Human
Conjugation PE

Mouse IgG2a (gamma 2a chain specific) rat monoclonal antibody, clone SB84a, PE

Applications Flow Cytometry:  ≤ 0.2 µg/106 cells.
Identification and enumeration of IgG2a+.
Immunofluorescence Microscopy: Identification and enumeration of IgG2a+.
ELISA/FLISA.
Also used as a second step reagent for mouse IgG2a monoclonal antibodies.
Reactivities Mouse
Conjugation PE

Human IgG (Fd region) mouse monoclonal antibody, clone HP6045, PE

Applications ELISA (both capture and detection).
Western Blot.
Radioimmunoassay
Reactivities Human
Conjugation PE

Human IgG2 (Fc specific) mouse monoclonal antibody, clone 31-7-4, PE

Applications ELISA.
Immunofluorescence < / = 0.2 µg/10e6 cells.
Reactivities Human
Conjugation PE

Human IgG (gamma chain specific) mouse monoclonal antibody, clone JDC-10, PE

Applications Indirect Immunofluorescent staining of IgG+ human B lymphocytes: < / = 0.2 µg/10e6 cells.
Reactivities Human
Conjugation PE

Chicken IgA (alpha chain specific) mouse monoclonal antibody, clone A-1, PE

Applications Flow Cytometry: < / = 0.2 µg/10e6 cells.
Immunofluorescence.
Reactivities Chicken
Conjugation PE

Rat IgG2b (Gamma-2b chain specific) mouse monoclonal antibody, clone 2B 10A8, PE

Applications Flow Cytometry: < / = 0.02 µg/10e6 cells.
Immunofluorescence Microscopy: Identification and enumeration of IgG2b+ cells.
Reactivities Rat
Conjugation PE

Rat IgG1 (gamma 1 chain specific) mouse monoclonal antibody, clone G1 7E7, PE

Applications Flow Cytometry: < / = 0.02 µg/10e6 cells.
Immunofluorescence Microscopy: Identification and enumeration of IgG1+ cells.
Reactivities Rat
Conjugation PE

Rat IgG1 (gamma 1 chain specific) mouse monoclonal antibody, clone G1 1C5, PE

Applications Flow Cytometry: < / = 0.02 µg/10e6 cells.
Immunofluorescence Microscopy: Identification and enumeration of IgG1+ cells.
Reactivities Rat
Conjugation PE

Mouse IgA (alpha chain specific) rat monoclonal antibody, clone 11-44-2, PE

Applications Flow Cytometry: < / = 0.2 µg/10e6 cells.
Identification and enumeration of IgA+ cells.
Immunofluorescence Microscopy: Identification and enumeration of IgA+ cells.
Reactivities Mouse
Conjugation PE

Mouse IgA (alpha chain specific) rat monoclonal antibody, clone 11-44-2, PE

Applications Flow Cytometry: < / = 0.2 µg/10e6 cells.
Identification and enumeration of IgA+ cells.
Immunofluorescence Microscopy: Identification and enumeration of IgA+ cells.
Reactivities Mouse
Conjugation PE

Mouse IgE (Epsilon heavy chain) rat monoclonal antibody, clone 23G3, PE

Applications Flow Cytometry: < / = 0.2 µg/10e6 cells.
Identification and enumeration of IgE+ cells.
Immunofluorescence Microscopy: Identification and enumeration of IgE+ cells.
Reactivities Mouse
Conjugation PE

Mouse IgG (F(ab)2 specific), F(ab)2 Fragment, adsorbed goat polyclonal antibody, PE

Applications Suitable for Immunomicroscopy (1/100-1/250) and Flow Cytometry (1/100-1/250) or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.
Recommended Dilutions: The maximum amount of reagent required to stain 1 x 10e6 cells in Flow Cytometry is approximately 1.0 µg of antibody conjugate. Lesser amounts of reagent may be sufficient for staining. As a general guideline dilutions of 1/50 to 1/200 should be suitable for most applications.
Reactivities Mouse
Conjugation PE

Mouse IgG2a goat polyclonal antibody, PE

Applications Flow Cytometry: 1/20 - 1/30.
Reactivities Mouse
Conjugation PE