Secondary Antibodies

Mouse IgA+IgG+IgM (H+L chain) goat polyclonal antibody, PE

Applications FLISA: ? 0.1 µg/ml
Flow Cytometry: ? 0.1 µg/106 cells.
Reactivities Mouse
Conjugation PE

Mouse IgA+IgG+IgM (H+L chain) goat polyclonal antibody, PE

Applications FLISA: ? 0.1 µg/ml
Flow Cytometry: ? 0.1 µg/106 cells.
Reactivities Mouse
Conjugation PE

Mouse IgM (Mu chain specific) rat monoclonal antibody, clone 1B4B1, PE

Applications Flow Cytometry: < / = 0.2 µg/10e6 cells.
Identification and enumeration of IgE+ cells.
Immunofluorescence Microscopy: Identification and enumeration of IgE+ cells.
Also used as a second step reagent for mouse IgM monoclonal antibodies.
Reactivities Mouse
Conjugation PE

Mouse IgM (Mu chain specific) rat monoclonal antibody, clone 1B4B1, PE

Applications Flow Cytometry: < / = 0.2 µg/10e6 cells.
Identification and enumeration of IgE+ cells.
Immunofluorescence Microscopy: Identification and enumeration of IgE+ cells.
Also used as a second step reagent for mouse IgM monoclonal antibodies.
Reactivities Mouse
Conjugation PE

Mouse IgA (alpha chain specific) goat polyclonal antibody, PE

Applications FLISA.
Flow Cytometry.
Immunofluorescence:
≤ 0.1 µg/106 cells.
Immunohistochemistry.
Reactivities Mouse
Conjugation PE

Mouse IgE (Epsilon heavy chain) rat monoclonal antibody, clone 23G3, PE

Applications Flow Cytometry: < / = 0.2 µg/10e6 cells.
Identification and enumeration of IgE+ cells.
Immunofluorescence Microscopy: Identification and enumeration of IgE+ cells.
Reactivities Mouse
Conjugation PE

Mouse IgG2a (gamma 2a chain specific) rat monoclonal antibody, clone SB84a, PE

Applications Flow Cytometry: ≤ 0.2 µg/106 cells.
Identification and enumeration of IgG2a+.
Immunofluorescence Microscopy: Identification and enumeration of IgG2a+.
ELISA/FLISA.
Also used as a second step reagent for mouse IgG2a monoclonal antibodies.
Reactivities Mouse
Conjugation PE

Mouse IgG2a (gamma 2a chain specific) rat monoclonal antibody, clone SB84a, PE

Applications Flow Cytometry: ≤ 0.2 µg/106 cells.
Identification and enumeration of IgG2a+.
Immunofluorescence Microscopy: Identification and enumeration of IgG2a+.
ELISA/FLISA.
Also used as a second step reagent for mouse IgG2a monoclonal antibodies.
Reactivities Mouse
Conjugation PE

Mouse IgG (F(ab)2 specific), F(ab)2 Fragment, adsorbed goat polyclonal antibody, PE

Applications Suitable for Immunomicroscopy (1/100-1/250) and Flow Cytometry (1/100-1/250) or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.
Recommended Dilutions: The maximum amount of reagent required to stain 1 x 10e6 cells in Flow Cytometry is approximately 1.0 µg of antibody conjugate. Lesser amounts of reagent may be sufficient for staining. As a general guideline dilutions of 1/50 to 1/200 should be suitable for most applications.
Reactivities Mouse
Conjugation PE

Mouse IgE (Epsilon heavy chain) rat monoclonal antibody, clone 23G3, PE

Applications Flow Cytometry: < / = 0.2 µg/10e6 cells.
Identification and enumeration of IgE+ cells.
Immunofluorescence Microscopy: Identification and enumeration of IgE+ cells.
Reactivities Mouse
Conjugation PE

Mouse IgA (alpha chain specific) rat monoclonal antibody, clone 11-44-2, PE

Applications Flow Cytometry: < / = 0.2 µg/10e6 cells.
Identification and enumeration of IgA+ cells.
Immunofluorescence Microscopy: Identification and enumeration of IgA+ cells.
Reactivities Mouse
Conjugation PE

Mouse IgA (alpha chain specific) rat monoclonal antibody, clone 11-44-2, PE

Applications Flow Cytometry: < / = 0.2 µg/10e6 cells.
Identification and enumeration of IgA+ cells.
Immunofluorescence Microscopy: Identification and enumeration of IgA+ cells.
Reactivities Mouse
Conjugation PE

Mouse IgG (H+L chain), F(ab)2 Fragment, adsorbed rabbit polyclonal antibody, PE

Applications Immunofluorescence: 1/100 - 1/250
Flow Cytometry: 1/100 - 1/250; The maximum amount of reagent required to stain 1 x 10e6 cells in flow cytometry is approximately 1.0 µg of antibody conjugate. Lesser amounts of reagent may be sufficient for staining.

Optimal titers for other applications should be determined by the researcher. As a general guideline dilutions of 1/100 to 1/250 should be suitable for most applications.
Reactivities Mouse
Conjugation PE

Mouse IgG (Fc specific), F(ab)2 Fragment, adsorbed goat polyclonal antibody, PE

Applications Suitable for Immunomicroscopy and Flow Cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity. The maximum amount of reagent required to stain 1 x 106 cells in Flow Cytometry is approximately 1.0 µg of antibody conjugate. Lesser amounts of reagent may be sufficient for staining. As a general guideline dilutions of 1/100 to 1/250 should be suitable for most applications.
Recommended Dilutions:
Immunofluorescence: 1/100-1/250.
Flow Cytometry: 1/100-1/250.
Reactivities Mouse
Conjugation PE

Mouse IgG (H+L chain), adsorbed goat polyclonal antibody, PE

Applications Flow Cytometry: Use 50 µl of 1/10 diluted antibody to label 10e6 cells in 100 µl.
Reactivities Mouse
Conjugation PE