Secondary Antibodies

Mouse IgM (Fc specific) rabbit polyclonal antibody, TRITC

Applications ELISA.
Immunocytochemistry.
(In)direct immunofluorescence

Immunohistochemistry on Frozen Sections.
Direct immunofluorescence staining of cytoplasmic Ig of appropriately treated cell and tissue substrates; to demonstrate immunoglobulins or specific antibodies in cells and tissues; to identify circulating antibodies in serodiagnostic microbiology and autoimmune diseases. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Recommended Working Dilutions: 1/10- 1/80.
Reactivities Mouse
Conjugation TRITC

Mouse IgG3 Antibody Rhodamine Conjugated

Applications WB: 1:2,000 - 1:10,000
IHC: 1:1,000 - 1:5,000
IF: 1:1,000 - 1:5,000
ELISA: 1:2,000 - 1:10,000
FLISA: 1:20,000 - 1:100,000
Reactivities Mouse
Conjugation TRITC

Mouse IgA (alpha chain specific) goat polyclonal antibody, TRITC

Applications FLISA: 1/100-1/400.
Flow Cytometry.
Immunohistochemistry.
Reactivities Mouse
Conjugation TRITC

Mouse IgA+IgG+IgM (H+L chain) goat polyclonal antibody, TRITC

Applications

FLISA: 1/100–1/400.
Flow Cytometry.
Immunohistochemistry.

Reactivities Mouse
Conjugation TRITC

Rabbit IgG (H+L chain) goat polyclonal antibody, TRITC, Lyophilized

Applications Suitable for Immunomicroscopy and Flow Cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
Recommended Dilutions:
FLISA: 1/10,000-1/50,000.
IF Microscopy: 1/1,000-1/5,000.
Reactivities Rabbit
Conjugation TRITC

Human IgA (Fc specific) sheep polyclonal antibody, TRITC

Applications Direct staining of fixed cell and tissue substrates, to demonstrate the intracellular presence of IgA. The absence of the Fc domain in the conjugate ensures minimal interaction with the tissue components and cell surfaces other than the primary antibody activity. This conjugate is primarily intended for use in cell surface membrane staining procedures, to identify and quantitate Ig on B cells, especially if interference by Fc activity is expected.
Recommended Dilutions: 1/10-1/40
This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Reactivities Human
Conjugation TRITC

Human IgG (Fc specific) sheep polyclonal antibody, TRITC

Applications Direct staining of fixed cell and tissue substrates, to demonstrate the intracellular presence of IgG. The absence of the Fc domain in the conjugate ensures minimal interaction with the tissue components and cell surfaces other than the primary antibody activity. This conjugate is primarily intended for use in cell surface membrane staining procedures, to identify and quantitate Ig on B cells, especially if interference by Fc activity is expected.
Recommended Dilutions: 1/10-1/40
This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Reactivities Human
Conjugation TRITC

Human IgG (F(ab)2 specific) goat polyclonal antibody, TRITC

Applications Direct staining of fixed cell and tissue substrates, to demonstrate the intracellular presence of free of Ig
bound subunits of both kappa and lambda type. The absence of the Fc domain in the conjugate ensures minimal interaction with the tissue components and cell surfaces other than the primary antibody activity.
This conjugate is primarily intended for use in cell surface membrane staining procedures, to identify and quantitate Ig on B cells, especially if interference by Fc activity is expected.
Recommended Dilutions: 1/10-1/40
This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Reactivities Human
Conjugation TRITC

Mouse IgM (Fc specific) goat polyclonal antibody, TRITC

Applications Direct immunofluorescence staining of cytoplasmic Ig of appropriately treated cell and tissue substrates; to demonstrate immunoglobulins or specific antibodies in cells and tissues; to identify circulating antibodies in serodiagnostic microbiology and autoimmune diseases. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Working dilutions are usually between 1/10 and 1/80.
Reactivities Mouse
Conjugation TRITC

Human IgG (H&L) Antibody Rhodamine Conjugated

Applications IF: 1:500-1:2,500
Reactivities Human
Conjugation TRITC

F(ab')2 Sheep IgG (H&L) Antibody Rhodamine Conjugated

Applications IF: 1:500-1:2,500
Conjugation TRITC

Mouse IgG (gamma 1, 2a, 2b and 3 chain) Antibody Rhodamine Conjugated

Applications WB: 1:2,000 - 1:10,000
IHC: 1:1,000 - 1:5,000
IF: 1:1,000 - 1:5,000
FC: 1:1,000 - 1:5,000
FLISA: 1:20,000 - 1:100,000
Conjugation TRITC

F(ab')2 Rabbit IgG (H&L) Antibody Rhodamine Conjugated

Applications IF: 1:500-1:2,500
Conjugation TRITC

F(ab')2 Rabbit IgG Antibody Rhodamine Conjugated Pre-Adsorbed

Applications IF: 1:500-1:2,500
Conjugation TRITC

Chicken IgG (H&L) Antibody Rhodamine Conjugated Pre-Adsorbed

Applications IF: 1:1,000 - 1:5,000
FLISA: 1:10,000 - 1:50,000
Conjugation TRITC