Secondary Antibodies

Porcine IgM mouse monoclonal antibody, clone 206-2, Aff - Purified

Applications ELISA: 0.1 µg/ml (1/4000).
Immunoprecipitation.
Immunohistochemistry on Frozen Sections: 0.2 µg/ml (1/2000).
Immunohistochemistry on Paraffin Sections (Does not react on routinely processed Paraffin Section).
Does not react in Western Blot with reduced and denatured samples.
Reactivities Porcine
Conjugation Unconjugated

Monkey IgG (H&L) Antibody Biotin

Applications WB: 1:5,000 - 1:10,000
IHC: User Optimized
ELISA: 1:50,000 - 1:100,000
Conjugation Unconjugated

Human IgG4 mouse monoclonal antibody, clone MRQ-44, Purified

Applications Immunohistochemistry on Frozen and Formalin-Fixed, Paraffin-Embedded Sections: 1/100-1/500. 
Preparation and Pretreatment:
1. Cut 3-4 μm section of formalin-fixed paraffin-embedded tissue and place on positively charged slides; dry overnight at 58°C.
2. Deparaffinize, rehydrate, and epitope retrieve; the preferred method is the use of Heat Induced Epitope Retrieval (HIER) techniques in conjunction with a pressure cooker. The preferred method allows for simultaneous deparaffinization, rehydration, and epitope retrieval. Upon completion, rinse with 5 changes of distilled or deionized water.
3. If using HRP detection system, place slides in peroxide block for 10 minutes; rinse. If using AP detection system, omit this step.
Positive Control: Tonsil.
Reactivities Human
Conjugation Unconjugated

Human IgE mouse monoclonal antibody, clone I27/mAb27

Applications FACS surface, Western Blot, Basophil degranulation, Study of IgE binding to CD23 (Fc epsilon R2).
Usage recommendation
:
*This monoclonal antibody may be used between 1-10 μg/ml.
*Optimal dilution should be determined by each laboratory for each application.
*Coupled antibody: to maintain RT before use.
Reactivities Human
Conjugation Unconjugated

Human IgM mouse monoclonal antibody, clone M15/8, Purified

Applications Flow Cytometry: 20 µg/ml.
Immunohistochemistry on frozen sections: 20 µg/ml.
ELISA: 5 µg/ml as coating Antibody.
Immunoprecipitation.
Reactivities Human
Conjugation Unconjugated

Rabbit IgG (H&L) Antibody

Applications WB: 1:5,000 - 1:25,000
IHC: 1:1,000 - 1:5,000
ELISA: 1:50,000
Reactivities Rabbit
Conjugation Unconjugated

Mouse IgG (H&L) Antibody

Applications WB: 1:2,000 - 1:10,000
IHC: 1:1,000 - 1:5,000
ELISA: 1:20,000 - 1:100,000
Conjugation Unconjugated

Bovine IgG (H&L) Secondary Antibody

Applications WB: 1:2,000 - 1:10,000
IHC: 1:1,000 - 1:5,000
ELISA: 1:20,000 - 1:100,000
Conjugation Unconjugated

CNY 3,938.00

4周

Human IgG F(c) Antibody

Applications WB: 1:10,000 - 1:50,000
IHC: 1:1,000 - 1:5,000
ELISA: 1:20,000
Conjugation Unconjugated

Human (lambda chain) Antibody

Applications WB: 1:500 - 1:2,000
IHC: 1:1,000 - 1:5,000
ELISA: 1:4,000 - 1:20,000
Reactivities Human
Conjugation Unconjugated

Mouse IgG (H&L) Antibody Pre-adsorbed

Applications WB: 1:2,000 - 1:20,000
IHC: 1:1,000 - 1:5,000
ELISA: 1:50,000 - 1:100,000
Reactivities Mouse
Conjugation Unconjugated

Mouse IgM (mu chain) Antibody

Applications WB: 1:2,000 - 1:10,000
IHC: 1:1,000 - 1:5,000
ELISA: 1:20,400 - 1:40,400
Reactivities Mouse
Conjugation Unconjugated

CNY 2,231.00

4周

Goat IgG (H&L) Antibody

Applications WB: 1:2,000 - 1:10,000
IHC: 1:1,000 - 1:5,000
ELISA: 1:23,400-1:43,400
Reactivities Goat
Conjugation Unconjugated

Guinea Pig IgG (H&L) Antibody

Applications WB: 1:2,000 - 1:10,000
IHC: 1:1,000 - 1:5,000
ELISA: 1:15,000 - 1:75,000
Conjugation Unconjugated