Secondary Antibodies

Human IgD (Fc specific) goat polyclonal antibody, Azide Free

Applications This antiserum is intended to detect and identify IgD in serum or other body fluids using the Immunofixation technique. Like Immunoelectrophoresis Immunofixation is essentially a two step technique. Proteins in a complex mixture are separated by electrophoresis in a gel carrier, followed by immunoprecipitation in situ with the antiserum. Non-precipitated proteins are removed by washing and the precipitated complex is revealed with a protein stain which allows its exact localization.
Immunofixation may be the method of choice whenever a high level of sensitivity is required to identify a minor protein component against a high background of other proteins. It enables the detection and identification of more than one paraproteins in serum or of free light chain. The detection limit is approximately 0.5 to 1 mg/ml in the presence of normal levels of immunoglobulins.
Antibody titre: Precipitin titre not less than 1:16 when tested against an appropriate reference in agar immunodiffusion block titration.
Reactivities Human
Conjugation Unconjugated

Human IgG1 (gamma 1 chain specific) mouse monoclonal antibody, clone 4E3, Purified

Applications ELISA: ≤ 5 µg/ml (Ref.1)
Immunofluorescence: ≤ 1 µg/10e6 cells.
Flow Cytometry.
Reactivities Human
Conjugation Unconjugated

Human IgA (Secretory component) mouse monoclonal antibody, clone MC29/12, Purified

Applications Immunohistochemistry on frozen and paraffin sections.
ELISA.
Reactivities Human
Conjugation Unconjugated

Human IgM rat monoclonal antibody, clone HM-7, Aff - Purified

Applications ELISA.
Reactivities Human
Conjugation Unconjugated

Human IgG (Fc CH2 Domain specific) mouse monoclonal antibody, clone 8A4, Purified

Applications ELISA.
Flow cytometry (1:50/1:100): use 10 µl of the suggested working dilution to label 10e6 cells in 100 µl.
Reactivities Human
Conjugation Unconjugated

Human IgE mouse monoclonal antibody, clone MH25/1, Purified

Applications Immunofluorescence.
Immunohistochemistry on frozen and paraffin sections: 1/5 – 1/10 (Based on the Indirect method, with a normal ABC method the dilution can be 5-10 times higher, but the optimal dilution should be tested by serial dilution.) 
Dilute immediately before use with PBS or TRIS.
Localisation: D ε 1 epitope on Fc Fragment 
Positive control: Polypus nose
Reactivities Human
Conjugation Unconjugated

Human IgE mouse monoclonal antibody, clone 4G7, Purified

Applications ELISA.
Reactivities Human
Conjugation Unconjugated

Human IgD mouse monoclonal antibody, clone IA6-2, Purified

Applications Flow cytometry.
Reactivities Human
Conjugation Unconjugated

Human IgE mouse monoclonal antibody, clone B537M, Purified

Applications ELISA.
Reactivities Human
Conjugation Unconjugated

Human IgE mouse monoclonal antibody, clone 090-11348, Purified

Applications ELISA.
Recommended antibody pair for Sandwich Immunoassay:
Capture / Detection
AM00919PU-N / AM00918PU-N
Reactivities Human
Conjugation Unconjugated

Human IgM (Mu chain, Fab-Region) mouse monoclonal antibody, clone B1545M, Purified

Applications
This antibody can pair with itself in ELISA, EIA.
Can be used for specific IgM antibodies detection and for total IgM level detection.
Can also be used as a capture antibody for total IgM level detection.
Reactivities Human
Conjugation Unconjugated

Human IgA + IgG + IgM (light chain) mouse monoclonal antibody, clone 7A9

Reactivities Human
Conjugation Unconjugated

Human IgG2 (Fc specific) mouse monoclonal antibody, clone 52G1

Reactivities Human
Conjugation Unconjugated

Human IgE (C epsilon 3 specific) (in vitro) mouse monoclonal antibody, clone 4F4cc

Reactivities Human
Conjugation Unconjugated

Human IgE (Epsilon chain specific) mouse monoclonal antibody, clone HP6061, Purified

Applications ELISA: 2.5-10 µg/mL
This antibody is best used as a Capture antibody.
Reactivities Human
Conjugation Unconjugated