Secondary Antibodies

Duck IgM (Fc specific) goat polyclonal antibody, Azide Free

Applications As unlabelled primary or secondary reagent for indirect detection of IgM at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates.
Can be used to prepare conjugates of the user’s own choice, to prepare an insoluble Immunoaffinity adsorbent or a solid phase antibody reagent by coupling to an artificial carrier and as catching antibody in nonisotopic methodology and solid phase immunochemistry.
Recommended Dilutions:
ELISA and comparable non-precipitating antibody-binding assays: 1/200-1/4,000.
Immunohistochemistry: 1/50-1/250.
Note: When applied in any Cytochemical or Histochemical staining procedure or solid phase coupling technique, the optimum concentration of the IgG preparation should be established by titration before being used.
Antibody titre: Precipitin titre 1/16 when tested against pooled normal Duck serum in agar-block immunodiffusion titration.
Reactivities Duck
Conjugation Unconjugated

Duck IgG (H+L chain) rabbit polyclonal antibody, Azide Free

Applications ELISA.
Dot blot.
Immunoblotting.
Indirect Immunofluorescence.

Can be used as unlabelled primary or secondary reagent for indirect detection techniques, to prepare conjugates with markers of the user’s own choice, to prepare an insoluble immunoaffinity adsorbent or a solid phase antibody reagent by coupling to an artificial carrier and as catching or detection antibody in non-isotopic methodology and solid phase immunochemistry. When applied in any cytochemical or histochemical procedure or solids phase coupling technique, the optimum concentration of the IgG preparation should always be established by titration.
Recommended Working Dilutions:
Histochemistry: 1/100-1/500.
ELISA and comparable non-precipitating antibody-binding assays: 1/500-1/5,000.
Antibody titre: Precipitin titre not less than 1/128 when tested against normal Duck serum in agar block titration.
Reactivities Duck
Conjugation Unconjugated

Chicken IgG / Chicken IgY (heavy chain) mouse monoclonal antibody, clone 16C7, Purified

Applications Flow Cytometry.
ELISA.
Western Blot under non-reducing conditions.
Reactivities Duck
Conjugation Unconjugated

Chicken IgG / Chicken IgY mouse monoclonal antibody, clone 7C2, Purified

Applications Immunoassays.
Western blot (non-reduced): The typical starting working dilution is 1:10.
Also useful for immunopurification.
Reactivities Chicken, Duck
Conjugation Unconjugated