Secondary Antibodies

Human IgM (Mu chain specific) mouse monoclonal antibody, clone UHB, Purified

Applications Indirect Immunofluorescent staining of IgM+ human B lymphocytes: < / = 1 µg/10e6 cells.
ELISA.
Western blotting.
Dot- and Slot-Immunoblotting.
Immunohistochemistry on Frozen Sections.
Immunocytochemistry.
Reactivities Human
Conjugation Unconjugated

Human IgA (Fc specific) sheep polyclonal antibody, TRITC

Applications Direct staining of fixed cell and tissue substrates, to demonstrate the intracellular presence of IgA. The absence of the Fc domain in the conjugate ensures minimal interaction with the tissue components and cell surfaces other than the primary antibody activity. This conjugate is primarily intended for use in cell surface membrane staining procedures, to identify and quantitate Ig on B cells, especially if interference by Fc activity is expected.
Recommended Dilutions: 1/10-1/40
This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Reactivities Human
Conjugation TRITC

Human IgG (Fc specific) sheep polyclonal antibody, TRITC

Applications Direct staining of fixed cell and tissue substrates, to demonstrate the intracellular presence of IgG. The absence of the Fc domain in the conjugate ensures minimal interaction with the tissue components and cell surfaces other than the primary antibody activity. This conjugate is primarily intended for use in cell surface membrane staining procedures, to identify and quantitate Ig on B cells, especially if interference by Fc activity is expected.
Recommended Dilutions: 1/10-1/40
This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Reactivities Human
Conjugation TRITC

Human IgG (F(ab)2 specific) goat polyclonal antibody, FITC

Applications Direct staining of fixed cell and tissue substrates, to demonstrate the intracellular presence of free of Ig
bound subunits of both kappa and lambda type. The absence of the Fc domain in the conjugate ensures minimal interaction with the tissue components and cell surfaces other than the primary antibody activity.
This conjugate is primarily intended for use in cell surface membrane staining procedures, to identify and quantitate Ig on B cells, especially if interference by Fc activity is expected.
Recommended Dilutions: 1/20-1/80.
This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Reactivities Human
Conjugation FITC

Human IgG (F(ab)2 specific) goat polyclonal antibody, TRITC

Applications Direct staining of fixed cell and tissue substrates, to demonstrate the intracellular presence of free of Ig
bound subunits of both kappa and lambda type. The absence of the Fc domain in the conjugate ensures minimal interaction with the tissue components and cell surfaces other than the primary antibody activity.
This conjugate is primarily intended for use in cell surface membrane staining procedures, to identify and quantitate Ig on B cells, especially if interference by Fc activity is expected.
Recommended Dilutions: 1/10-1/40
This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Reactivities Human
Conjugation TRITC

Human Lambda Light Chain (free and bound) mouse monoclonal antibody, clone NI 412, NI 268, Azide Free

Applications To identify the light chain type of immunoglobulins or free light chains in Human serum, other body fluids, cell and tissue substrates and to determine its concentration in indirect techniques as ELISA, Indirect Immunoperoxidase staining and immunoblotting.
General Recommended Dilutions:
Histochemical Use: 1/50-1/200
ELISA: from 1/200 upwards
Western blot: from 1/400 upwards.
Reactivities Human
Conjugation Unconjugated

Human IgA (heavy chain) mouse monoclonal antibody, clone Mc24-2E11, Purified

Applications ELISA.
Western Blot.
Immunoprecipitation.
Immunohistochemistry on Frozen Sections.
Immunohistochemistry on Paraffin Sections (10 µg/ml).
Reactivities Human
Conjugation Unconjugated

Human IgD (F(ab)2 Fragment) rabbit polyclonal antibody, Purified

Applications ELISA
Reactivities Human
Conjugation Unconjugated

Human IgG (H&L) Antibody Rhodamine Conjugated

Applications IF: 1:500-1:2,500
Reactivities Human
Conjugation TRITC

Human IgM mouse monoclonal antibody, clone NI 179, Purified

Applications To identify the presence of IgM in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as radio immuno assay, ELISA, immunoblotting, immunoprecipitation, indirect immunoperoxidase and immunofluorescence
General Recommended Dilutions:
Histochemical use: 1/50-1/200
Dilutions may be prepared by adding PBS, pH 7.2.
Reactivities Human
Conjugation Unconjugated

Human IgM mouse monoclonal antibody, clone NI 179, Biotin

Applications To identify the presence of IgM in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as ELISA, Indirect immunoperoxidase and Indirect Immunofluorescence staining of cytoplasmic IgM, and Immunoblotting. As a second step an Avidin or Streptavidin conjugate have to be used.
General Recommended Dilutions:
Histochemical Use: 1/100-1/500
ELISA: from 1/1,000 upwards
Western blot: from 1/2,000 upwards
Reactivities Human
Conjugation Biotin

Human IgM mouse monoclonal antibody, clone NI 179, FITC

Applications To identify the presence of IgM in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as direct and indirect Immunofluorescence staining of cytoplasmic IgM and cell membrane IgM.
General Recommended Dilutions:
Histochemical Use: 1/10-1/50
Reactivities Human
Conjugation FITC

Human IgM mouse monoclonal antibody, clone NI 179, Ascites

Applications To identify the presence of IgM in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as ELISA, Indirect immunoperoxidase and Indirect Immunofluorescence staining of cytoplasmic IgM, and Immunoblotting. The optimum working
dilution is an assay-related characteristic and should always be determined by titration.
General Recommended Dilutions:
Histochemical Use: 1/50-1/200
ELISA: from 1/500 upwards
Western blot: from 1/1,000 upwards.
Reactivities Human
Conjugation Unconjugated

Human IgM mouse monoclonal antibody, clone NI 179, TRITC

Applications To identify the presence of IgM in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as direct and indirect immunofluorescence staining of cytoplasmic IgM and cell membrane IgM.
General Recommended Dilutions:
Histochemical Use: 1/10-1/100
Reactivities Human
Conjugation TRITC

Human IgG3 (F(ab)2 specific) mouse monoclonal antibody, clone NI 330 (HP 6050), TRITC

Applications To identify the presence of IgG3 in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as ELISA, indirect immunoperoxidase staining of cytoplasmic IgG3, and immunoblotting using a peroxidase labelled monoclonal antibody against TRITC.
General Recommended Dilutions:
Histochemical Use: 1/10-1/50.
ELISA: from 1/200 upwards.
Western blot: from 1/400 upwards.
Reactivities Human
Conjugation TRITC