Secondary Antibodies

Human IgG (H+L chain), adsorbed goat polyclonal antibody, Biotin

Applications Suitable for Immunoblotting, ELISA, Immunohistochemistry, Immunomicroscopy as well as other antibody based assays using streptavidin or avidin conjugates requiring lot-to-lot consistency.
Recommended Dilutions:
ELISA: 1/20,000-1/100,000.
Western blot: 1/2,000-1/10,000.
Immunohistochemistry: 1/1,000-1/5,000.
Note: This product has been assayed against 1.0 µg of Human IgG in a standard capture ELISA using Peroxidase Conjugated Streptavidin and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6- sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1/20,000 to 1/80,000 of the reconstitution concentration is suggested for this product.
Reactivities Human
Conjugation Biotin

Human IgG (H+L chain), adsorbed goat polyclonal antibody, FITC

Applications Suitable for Immunomicroscopy and Flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency. Other applications not tested.
Reactivities Human
Conjugation FITC

Human IgA + IgG + IgM (adsorbed F(ab)2 Fragment) goat polyclonal antibody, TRITC

Applications Suitable for Immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.
Reactivities Human
Conjugation TRITC

Human IgA + IgG + IgM (adsorbed F(ab)2 Fragment) goat polyclonal antibody, FITC

Applications Suitable for Immunomicroscopy and Flow Cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.
Reactivities Human
Conjugation FITC

Human IgA + IgG + IgM (adsorbed F(ab)2 Fragment) goat polyclonal antibody, AP

Applications Suitable for Immunoblotting (Western or dot blot), ELISA and immunohistochemistry as well as other phosphatase-antibody based enzymatic assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.
This product has been assayed against 1.0 ug of Human IgG in a standard capture ELISA using pNPP p-nitrophenyl phosphate as a substrate for 30 minutes at room temperature. A working dilution of 1:1,000 to 1:5,000 of the reconstitution concentration is suggested for this product.
Reactivities Human
Conjugation AP

Human IgA + IgG + IgM (adsorbed F(ab)2 Fragment) goat polyclonal antibody, Biotin

Applications ELISA: 1/20,000-1/100,000
Western Blot: 1/2,000-1/10,000
Immunohistochemistry: 1/1,000-1/5,000.
This product has been assayed against 1.0 µg of Human IgG in a standard capture ELISA using Peroxidase Conjugated Streptavidin (Cat.-No RA021HRP) and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6- sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1/10,000 to 1/50,000 of the reconstitution concentration is suggested for this product.
Reactivities Human
Conjugation Biotin

Human IgG (F(ab)2 specific), F(ab)2 Fragment, adsorbed goat polyclonal antibody, TRITC

Applications Suitable for Immunomicroscopy and Flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.
Reactivities Human
Conjugation TRITC

Human IgG (F(ab)2 specific), F(ab)2 Fragment, adsorbed goat polyclonal antibody, Texas Red

Applications Suitable for Immunomicroscopy and Flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.
Reactivities Human
Conjugation Texas Red

Human IgM (Fc5 mu specific), F(ab)2 Fragment goat polyclonal antibody, Texas Red

Applications Suitable for Immunomicroscopy and Flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.
Reactivities Human
Conjugation Texas Red

Human IgM (Fc5 mu specific), F(ab)2 Fragment goat polyclonal antibody, HRP

Applications Suitable for Immunoblotting (Western or dot blot), ELISA, Immunoperoxidase Electron Microscopy and Immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.
Recommended Dilutions:
ELISA: 1/100,000.
Western Blot: 1/2,000-1/10,000.
Immunhistochemistry: 1/1,000-1/5,000.
Reactivities Human
Conjugation HRP

Human IgM (Fc5 mu specific), F(ab)2 Fragment goat polyclonal antibody, Biotin

Applications Suitable for use in Immunoblotting, ELISA, Immunohistochemistry, Immunomicroscopy as well as other antibody based assays using streptavidin or avidin conjugates requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.
ELISA: 1:10,000 - 1:50,000.
Western Blot: 1:1,000 - 1:5,000.
Immunohistochemistry: 1:500 - 1:3,000.
Reactivities Human
Conjugation Biotin

Human IgM (Fc5 mu specific), F(ab)2 Fragment goat polyclonal antibody, FITC

Applications Suitable for Immunomicroscopy and Flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.
Flow Cytometry: 1:2,000-1:10,000.
Immunofluorescence microscopy: 1:500-1:2,500.
Reactivities Human
Conjugation FITC

Human IgA + IgG + IgM (adsorbed F(ab)2 Fragment) goat polyclonal antibody, Texas Red

Applications Suitable for Immunomicroscopy and Flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.
Reactivities Human
Conjugation Texas Red

Human IgG (adsorbed F(ab)2 Fragment) goat polyclonal antibody, TRITC

Applications Suitable for Immunomicroscopy and Flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.
Reactivities Human
Conjugation TRITC

Human IgG (adsorbed F(ab)2 Fragment) goat polyclonal antibody, Texas Red

Applications Suitable for Immunomicroscopy and Flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.
Reactivities Human
Conjugation Texas Red