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Secondary Antibodies
Human IgG (H+L chain), adsorbed rabbit polyclonal antibody, Aff - Purified
| Applications | Suitable for Immunoprecipitation, Immunodiffusion, Conjugation and most immunological methods requiring lot-to-lot consistency, high titer and specificity. Recommended Dilutions: ELISA: 1/75,000-1/500,000. Western Blot: 1/10,000-1/50,000. Immunohistochemistry: 1/1,000-1/10,000. |
| Reactivities | Human |
| Conjugation | Unconjugated |
CNY 3,188.00
4周
Human IgG (F(ab)2 specific), F(ab)2 Fragment, adsorbed goat polyclonal antibody, Aff - Purified
| Applications | Application(s): suitable for highly specific immunological methods requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity. Recommended Dilution(s): ELISA: 1:25,000 Western Blot: 1:1,000 - 1:5,000 Immunohistochemistry: 1:500 - 1:2,500 |
| Reactivities | Human |
| Conjugation | Unconjugated |
Human IgG3 (Hinge Region) mouse monoclonal antibody, clone HP6050, HRP
| Applications | ELISA: 1/4,000-1/10,000. Western blotting. Dot- and Slot-immunoblotting. Immunohistochemistry on Frozen Sections: 1/10-1/20. |
| Reactivities | Human |
| Conjugation | HRP |
CNY 3,940.00
5周
Human IgG (Fc heavy chain specific) mouse monoclonal antibody, clone EM-07, HRP
| Applications | ELISA: 0.1-0.5 µg/ml. Western blot. |
| Reactivities | Human |
| Conjugation | HRP |
CNY 4,400.00
4周
Human IgG1 (gamma 1 chain specific) mouse monoclonal antibody, clone 4E3, PE
| Applications | ELISA. FLISA (≤ 1.0 µg/ml). |
| Reactivities | Human |
| Conjugation | PE |
Human IgE (Fc specific) goat polyclonal antibody, HRP
| Applications | Enzyme-Immunocytochemical and Immunohistochemical staining for the detection of IgE at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates. To demonstrate circulating IgE antibodies in serodiagnostic microbiology and autoimmune diseases. To identify a specific antigen using an reference antibody of Human origin known to be of the IgE isotype in the middle layer of the indirect test procedure. In non-isotopic assay methodology (e.g. ELISA) to measure IgE in Human serum or other body fluids. Recommneded Dilutions: Histochemistry and Cytochemistry: 1/50-1/250. ELISA and comparable non-precipitating antibody-binding assays: 1/1,000-1/25,000. |
| Reactivities | Human |
| Conjugation | HRP |
Human IgE (Fc specific) goat polyclonal antibody, FITC
| Applications | Can be used in Immunocytochemical and Immunohistochemical staining for the detection of IgE at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates, to identify and measure IgE in Human serum or other body fluids. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Recommended Working Dilutions: 1/20-1/80, depending on the method used. |
| Reactivities | Human |
| Conjugation | FITC |
Goat Polyclonal Anti-Human IgG Antibody
| Applications | WB 1:500-1:2,000 |
| Reactivities | Human |
| Conjugation | Unconjugated |
CNY 6,190.00
5周
Human IgA (Secretory component) mouse monoclonal antibody, clone NI 194-4 (A89-039), Ascites
| Applications | To identify the presence of free or bound Secretory component in Human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as Radioimmunoassay, ELISA, Indirect Immunoperoxidase and Immunofluorescence staining, Haemagglutination and Immunoblotting. General Recommended Dilutions: Histochemical Use: from 1/50-1/200. ELISA: form 1/500 upwards. Western blot: from 1/1000 upwards. |
| Reactivities | Human |
| Conjugation | Unconjugated |
CNY 9,630.00
5周
Human IgA (Secretory component) mouse monoclonal antibody, clone NI 194-4 (A89-039), HRP
| Applications | To identify the presence of free or bound Secretory component in Human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as Radioimmunoassay, ELISA, Indirect Immunoperoxidase and Immunofluorescence staining, Haemagglutination and Immunoblotting. General Recommended Dilutions: Histochemical Use: from 1/20 upwards. ELISA: form 1/50 upwards. Western blot: from 1/100 upwards. |
| Reactivities | Human |
| Conjugation | HRP |
CNY 9,630.00
5周
Human IgA (Secretory component) mouse monoclonal antibody, clone NI 194-4 (A89-039), Biotin
| Applications | To identify the presence of free or bound Secretory component in Human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as Radioimmunoassay, ELISA, Indirect Immunoperoxidase and Immunofluorescence staining, Haemagglutination and Immunoblotting. General Recommended Dilutions: Histochemical Use: from 1/20 upwards ELISA: form 1/25 upwards Western blot: from 1/50 upwards. |
| Reactivities | Human |
| Conjugation | Biotin |
Human IgE mouse monoclonal antibody, clone NI 307, FITC
| Applications | To identify the presence of IgE in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as direct and indirect immunofluorescence staining of cytoplasmic IgE and cell membrane IgE. General Recommended Dilutions: Histochemical Use: 1/50-1/200. |
| Reactivities | Human |
| Conjugation | FITC |
Human IgM (Mu chain, Fab-Region) mouse monoclonal antibody, clone MA2, Purified
| Applications | ELISA. |
| Reactivities | Human |
| Conjugation | Unconjugated |
Human IgG4 mouse monoclonal antibody, clone MRQ-44, Purified
| Applications | Immunohistochemistry on Frozen and Formalin-Fixed, Paraffin-Embedded Sections: 1/100-1/500. Preparation and Pretreatment: 1. Cut 3-4 μm section of formalin-fixed paraffin-embedded tissue and place on positively charged slides; dry overnight at 58°C. 2. Deparaffinize, rehydrate, and epitope retrieve; the preferred method is the use of Heat Induced Epitope Retrieval (HIER) techniques in conjunction with a pressure cooker. The preferred method allows for simultaneous deparaffinization, rehydration, and epitope retrieval. Upon completion, rinse with 5 changes of distilled or deionized water. 3. If using HRP detection system, place slides in peroxide block for 10 minutes; rinse. If using AP detection system, omit this step. Positive Control: Tonsil. |
| Reactivities | Human |
| Conjugation | Unconjugated |
