Human IgE mouse monoclonal antibody, clone I27/mAb27
| Applications | FACS surface, Western Blot, Basophil degranulation, Study of IgE binding to CD23 (Fc epsilon R2). Usage recommendation: *This monoclonal antibody may be used between 1-10 μg/ml. *Optimal dilution should be determined by each laboratory for each application. *Coupled antibody: to maintain RT before use. |
| Reactivities | Human |
| Conjugation | Unconjugated |
Human IgM mouse monoclonal antibody, clone M15/8, Purified
| Applications | Flow Cytometry: 20 µg/ml. Immunohistochemistry on frozen sections: 20 µg/ml. ELISA: 5 µg/ml as coating Antibody. Immunoprecipitation. |
| Reactivities | Human |
| Conjugation | Unconjugated |
Human IgA (Secretory component) goat polyclonal antibody, FITC
| Applications | Can be used as reagent for the direct detection of secretory component in human cells, tissues and body fluids in Immunofluorescence, as detection reagent in non-isotopic methodology and solid phase immunochemistry (e.g. ELISA). This immunoconjugate is not pre-diluted and the optimum working dilution should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Working Dilutions: 1/20-1/80. |
| Reactivities | Human |
| Conjugation | FITC |
Human IgD (Fc specific) goat polyclonal antibody, HRP
| Applications | Can be used in Enzyme-immunocytochemical and Immunohistochemical staining for the detection of IgD at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates; to demonstrate circulating IgD antibodies in serodiagnostic microbiology and autoimmune diseases; in nonisotopic assay methodology (e.g. ELISA) to measure IgD in human serum or other body fluids. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Recommended working dilutions: Histochemical and Cytochemical: 1/50 - 1/500. ELISA and comparable non-precipitating antibody-binding assays: 1/500 - 1/10,000. |
| Reactivities | Human |
| Conjugation | HRP |
Human IgD (Fc specific) goat polyclonal antibody, Biotin
| Applications | Can be used in Immunocytochemical and Immunohistochemical staining for the detection of IgD at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates, and to demonstrate circulating antibodies in serodiagnostic microbiology. In non-isotopic assay methodology (e.g. ELISA) to identify and measure IgD in human serum or other body fluid. As a second step an avidin or streptavidin conjugate of the user’s choice has to be used. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Recommended Dilutions: Histochemical and Cytochemical Use: 1/100-1/250 ELISA and comparable non-precipitating antibody-binding assays: 1/1,000-1/10,000. Performance testing. Isotype specificity and quantitative specific recognition ability are further evaluated at the high level of sensitivity by direct singe and simultaneous double staining of different types of human cells, using counterstaining with reference conjugates with a different marker. The immunoconjugate is further tested in ELISA-type assays. |
| Reactivities | Human |
| Conjugation | Biotin |
Human IgG F(c) Antibody Biotin Conjugated Pre-Adsorbed
| Applications | WB: 1:1,000 - 1:5,000 IHC: 1:500 - 1:2,500 ELISA: 1:194,000 - 1:294,000 |
| Reactivities | Human |
| Conjugation | Biotin |
Human (lambda chain) Antibody
| Applications | WB: 1:500 - 1:2,000 IHC: 1:1,000 - 1:5,000 ELISA: 1:4,000 - 1:20,000 |
| Reactivities | Human |
| Conjugation | Unconjugated |
Human IgG (H&L) Antibody Alkaline Phosphatase Conjugated Pre-Adsorbed
| Applications | WB: 1:500 - 1:2,500 IHC: 1:200 - 1:1,000 ELISA: 1:2,000 - 1:10,000 |
| Reactivities | Human |
| Conjugation | Alkaline Phosphatase |
Human IgG (H&L) Antibody Peroxidase Conjugated
| Applications | WB: 1:1,000 - 1:5,000 IHC: 1:500 - 1:2,500 ELISA: 1:10,000 - 1:50,000 |
| Reactivities | Human |
| Conjugation | HRP |
Human IgG (H&L) Antibody Peroxidase Conjugated
| Applications | WB: 1:1,000 - 1:10,000 IHC: 1:500 - 1:2,500 ELISA: 1:150,000 - 1:250,000 |
| Reactivities | Human |
| Conjugation | HRP |
Human IgG F(c) Antibody Pre-Adsorbed
| Applications | WB: 1:1,000 - 1:5,000 IHC: 1:1,000 - 1:3,000 ELISA: 1:15,000 - 1:60,000 |
| Reactivities | Human |
| Conjugation | Unconjugated |
Human IgG F(c) Antibody
| Applications | WB: 1:2,000 - 1:10,000 IHC: 1:1,000 - 1:5,000 ELISA: 1:20,000 - 1:100,000 |
| Reactivities | Human |
| Conjugation | Unconjugated |
Human IgG (H&L) Antibody
| Applications | WB: 1:500 - 1:5,000 IHC: 1:1,000 - 1:5,000 ELISA: 1:5,000 - 1:50,000 |
| Reactivities | Human |
| Conjugation | Unconjugated |
Human IgG (H&L) Antibody Biotin Conjugated Pre-Adsorbed
| Applications | WB: 1:2,000 - 1:10,000 IHC: 1:1,000 - 1:5,000 ELISA: 1:20,000 - 1:100,000 |
| Reactivities | Human |
| Conjugation | Biotin |
Human IgG (H&L) Antibody Alkaline Phosphatase Conjugated
| Applications | WB: 1:500 - 1:2,500 IHC: 1:200 - 1:1,000 ELISA: 1:2,000 - 1:10,000 |
| Reactivities | Human |
| Conjugation | Alkaline Phosphatase |
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