Secondary Antibodies

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Mouse IgE (Fc specific) Rabbit Polyclonal Antibody
AP21485BT-N

CNY 7,710.00

5周

Mouse IgE (Fc specific) rabbit polyclonal antibody, Biotin

Applications Can be used in Immunocytochemical and Immunohistochemical staining for the detection of IgE at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates, and to demonstrate circulating antibodies in serodiagnostic microbiology. In non-isotopic assay methodology (e.g. ELISA) to identify and measure IgE in mouse serum or other body fluid. As a second step an avidin or streptavidin conjugate of the user’s choice has to be used. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Recommended Working Dilutions:
Histochemical and Cytochemical: 1/100-1/250.
ELISA and comparable non-precipitating antibody-binding assays: 1/500-1/3000.
Performance testing: Isotype specificity and quantitative specific recognition ability are further evaluated at the high level of sensitivity by direct singe and simultaneous double staining of different types of mouse cells, using counterstaining with reference conjugates with a different marker. The immunoconjugate is further tested in ELISA-type assays.
Reactivities Mouse
Conjugation Biotin
Mouse IgM (Fc specific) Goat Polyclonal Antibody
AP31543HR-N

CNY 7,710.00

5周

Mouse IgM (Fc specific) goat polyclonal antibody, HRP

Applications Can be used in enzyme-immunocytochemical and immunohistochemical staining for the detection of IgM at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates; to demonstrate circulating IgM antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen using a reference antibody of mouse origin known to be of the IgM isotype in the middle layer of the indirect test procedure; in non-isotopic assay methodology (e.g. ELISA) to measure IgM in mouse serum or other body fluids. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Working dilutions:
For histochemical and cytochemical use are usually between 1/100 and 1/400.
In ELISA and comparable non-precipitating antibody-binding assays between 1/1000 and 1/8000 depending on the method used.
Reactivities Mouse
Conjugation HRP
Mouse IgM (Fc specific) Rabbit Polyclonal Antibody
AP32992HR-N

CNY 7,710.00

5周

Mouse IgM (Fc specific) rabbit polyclonal antibody, HRP

Applications ELISA.
Dot blot.
Immunoblotting.
Immunocytochemistry.
Immunohistochemistry on Paraffin Sections.
In enzyme-immunocytochemical and immunohistochemical staining for the detection of IgM at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates; to demonstrate circulating IgM antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen using a reference antibody of mouse origin known to be of the IgM isotype in the middle layer of the indirect test procedure; in non-isotopic assay methodology (e.g. ELISA) to measure IgM in mouse serum or other body fluids. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Recommended Working Dilutions
Histochemical and cytochemical Use: 1/100- 1/500.
ELISA and comparable non-precipitating antibody-binding assays: 1/1,000- 1/5,000.
Reactivities Mouse
Conjugation HRP
Mouse IgM (Fc specific) Rabbit Polyclonal Antibody
AP32992BT-N

CNY 7,710.00

5周

Mouse IgM (Fc specific) rabbit polyclonal antibody, Biotin

Applications In immunocytochemical and immunohistochemical staining of IgM at the cellular and subcellular level of appropriately treated cell and tissue substrates; to demonstrate circulating IgM antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen using a reference antibody of mouse origin known to be of the IgM isotype in the middle layer of the indirect test procedure; in non-isotopic assay methodology (e.g. ELISA) to measure IgM in mouse serum or other body fluids. As a second step an avidin or streptavidin conjugate of the user’s choice has to be used. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Recommended Working Dilutions:
Histochemical and cytochemical Use: 1/100- 1/2500.
ELISA and comparable non-precipitating antibody-binding assays: 1/1000- 1/5000.
Reactivities Mouse
Conjugation Biotin
Mouse IgG (H+L chain) Sheep Polyclonal Antibody
R1256TR

CNY 2,657.00

4周

Mouse IgG (H+L chain) sheep polyclonal antibody, Texas Red

Applications Suitable for Immunomicroscopy and Flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
Reactivities Mouse
Conjugation Texas Red
Mouse IgG (H+L chain) Sheep Polyclonal Antibody
R1256T

CNY 2,520.00

4周

Mouse IgG (H+L chain) sheep polyclonal antibody, TRITC

Applications Suitable for Immunomicroscopy and Flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
Reactivities Mouse
Conjugation TRITC
Mouse IgG (H+L chain) Sheep Polyclonal Antibody
R1256F

CNY 2,520.00

4周

Mouse IgG (H+L chain) sheep polyclonal antibody, FITC

Applications Suitable for Immunomicroscopy and Flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
Reactivities Mouse
Conjugation FITC
Mouse IgG (H+L chain) Sheep Polyclonal Antibody
R1256AP

CNY 3,224.00

4周

Mouse IgG (H+L chain) sheep polyclonal antibody, AP

Applications Suitable for Immunoblotting (Western or Dot blot), ELISA and Immunohistochemistry as well as other phosphatase-antibody based enzymatic assays requiring lot-to-lot consistency.
Recommended dilutions: This product has been assayed against 1.0 �g of Mouse IgG in a standard capture ELISA using pNPP p-nitrophenyl phosphate as a substrate for 30 minutes at room temperature. A working dilution of 1:1,000 to 1:5,000 of the reconstitution concentration is suggested for this product.
Reactivities Mouse
Conjugation AP
Mouse IgG (H+L chain) Sheep Polyclonal Antibody
R1256B

CNY 2,657.00

4周

Mouse IgG (H+L chain) sheep polyclonal antibody, Biotin

Applications Suiatble for Immunoblotting, ELISA, Immunohistochemistry, Immunomicroscopy as well as other antibody based assays using streptavidin or avidin conjugates requiring lot-to-lot consistency.
Recommended Dilutions:
ELISA: 1/10,000-1/50,000.
Western Blot: 1/1,000-1/5,000.
Immunohistochemistry: 1/1,000-1/2,000.
Reactivities Mouse
Conjugation Biotin
Mouse IgG (Fc specific) Rabbit Polyclonal Antibody
R1352P

CNY 2,657.00

4周

Mouse IgG (Fc specific) rabbit polyclonal antibody, Aff - Purified

Applications Immunoprecipitation.
Immunodiffusion.
Conjugation and most immunological methods requiring lot-to-lot consistency, high titer and specificity.
Recommended dilutions:
ELISA: 1/40,000-1/180,000.
Western blot: 1/2,000-1/10,000.
Immunohistochemistry: 1/1,000-1/5,000.
Reactivities Mouse
Conjugation Unconjugated
Mouse IgG1 (Gamma 1 chain) ATTO 532 Conjugated Antibody
TA397871

CNY 5,888.00

4周

Mouse IgG1 (Gamma 1 chain) ATTO 532 Conjugated Antibody

Applications WB: >1:10,000
IF: >1:5,000
FLISA: >1:20,000
Reactivities Mouse
Conjugation ATTO 532
Mouse IgG1 (Gamma 1 chain) ATTO 594 Conjugated Antibody
TA397881

CNY 5,888.00

4周

Mouse IgG1 (Gamma 1 chain) ATTO 594 Conjugated Antibody

Applications WB: >1:10,000
IF: >1:5,000
FLISA: >1:20,000
Reactivities Mouse
Conjugation ATTO 594
Mouse IgG1 (Gamma 1 chain) ATTO 655 Conjugated Antibody
TA397891

CNY 5,888.00

4周

Mouse IgG1 (Gamma 1 chain) ATTO 655 Conjugated Antibody

Applications WB: >1:10,000
IF: >1:5,000
FLISA: >1:20,000
Reactivities Mouse
Conjugation ATTO 647N
Mouse IgG1 (Gamma 1 chain) ATTO 488 Conjugated Antibody
TA397866

CNY 5,888.00

4周

Mouse IgG1 (Gamma 1 chain) ATTO 488 Conjugated Antibody

Applications WB: >1:10,000
IF: >1:5,000
FLISA: >1:20,000
Reactivities Mouse
Conjugation ATTO 488
Mouse IgG1 (Gamma 1 chain) ATTO 425 Conjugated Antibody
TA397860

CNY 5,888.00

4周

Mouse IgG1 (Gamma 1 chain) ATTO 425 Conjugated Antibody

Applications WB: >1:10,000
IF: >1:5,000
FLISA: >1:20,000
Reactivities Mouse
Conjugation ATTO 425