Antibodies

Human IgG (Isotype Specific) mouse monoclonal antibody, clone NI 335 and NI 343, Purified

Applications To identify the presence of IgG in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as ELISA, indirect immunoperoxidase and indirect immunofluorescence staining of
cytoplasmic IgG, and immunoblotting.
General Recommended Dilutions:
Histochemical Use: 1/50-1/200
ELISA: from 1/500 upwards
Western blot: from 1/1000 upwards.
Reactivities Human
Conjugation Unconjugated

Human IgG (Fc heavy chain specific) mouse monoclonal antibody, clone EM-07, FITC

Applications Direct Immunofluorescence analysis: 2-5 µg/ml.
Reactivities Human
Conjugation FITC

Human IgE mouse monoclonal antibody, clone 4H10, FITC

Applications Flow cytometry: 2-5 µg/ml.
Reactivities Human
Conjugation FITC

Human IgE mouse monoclonal antibody, clone 4H10, Purified

Applications Flow Cytometry: 4 µg/ml.
ELISA.
Western Blot.
Reactivities Human
Conjugation Unconjugated

Human IgG (Fd region) mouse monoclonal antibody, clone HP6045, PE

Applications ELISA (both capture and detection).
Western Blot.
Radioimmunoassay
Reactivities Human
Conjugation PE

Human IgG (Fd region) mouse monoclonal antibody, clone HP6045, Biotin

Applications ELISA (both capture and detection).
Western Blot.
Radioimmunoassay
Reactivities Human
Conjugation Biotin

Human IgG (Fd region) mouse monoclonal antibody, clone HP6045, FITC

Applications ELISA (both capture and detection).
Western Blot.
Radioimmunoassay
Reactivities Human
Conjugation FITC

Goat IgG (H+L chain) mouse polyclonal antibody, Serum

Applications ELISA: 1/20,000-1/100,000.
Western Blot: 1/2,000-1/10,000.
Immunochemistry: 1/1,000-1/5,000.
Reactivities Goat
Conjugation Unconjugated

Rat IgM (Mu chain specific) mouse monoclonal antibody, Biotin

Applications Flow Cytometry (< / = 1.0 µg/10e6 cells).
Immunofluorescence Microscopy: Identification and enumeration of IgM+ cells.
ELISA.
Second step reagent for rat IgM Monoclonal Antibodies.
Surface IgM Detection.
B Cell Stimulation.
Reactivities Rat
Conjugation Biotin

Chicken IgG / Chicken IgY mouse monoclonal antibody, clone 4G12, Purified

Applications Western Blot: 0.5 µg/ml for HRPO/ECL detection.
Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubation buffer.
ELISA: 0.1 µg/ml.
Immunoprecipitation: 1 - 10 µg.
Reactivities Chicken
Conjugation Unconjugated

Human IgG (heavy chain) mouse monoclonal antibody, clone B33/20, Purified

Applications ELISA.
Reactivities Human
Conjugation Unconjugated

Chicken IgG / Chicken IgY (heavy chain) mouse monoclonal antibody, clone 16C7, Purified

Applications Flow Cytometry.
ELISA.
Western Blot under non-reducing conditions.
Reactivities Duck
Conjugation Unconjugated

Human IgG3 (Hinge Region) mouse monoclonal antibody, clone HP6050, PE

Applications FLISA: 1 µg/ml.
Immunocytochemistry.
Immunohistochemistry on Frozen Sections.
Reactivities Human
Conjugation PE

Human IgG3 (Hinge Region) mouse monoclonal antibody, clone HP6050, FITC

Applications FLISA: 1/200-1/400
Immunocytochemistry.
Immunohistochemistry on Frozen Sections.
Reactivities Human
Conjugation FITC