Antibodies

Bovine IgM (Fc specific) rabbit polyclonal antibody, HRP

Applications Enzyme-Immunocytochemical and Immunohistochemical staining for the detection of IgM at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates.
To demonstrate circulating IgM antibodies in serodiagnostic microbiology and autoimmune diseases.
To identify a specific antigen using an reference antibody of bovine origin known to be of the IgM isotype in the middle layer of the indirect test procedure.
In non-isotopic assay methodology (e.g. ELISA) to measure IgM in Bovine serum or other body fluids.
Recommneded Dilutions:
Histochemistry and Cytochemistry: 1/50-1/250.
ELISA and comparable non-precipitating antibody-binding assays: 1/500-1/2,000.
Reactivities Bovine
Conjugation HRP

Bovine IgM (Fc specific) rabbit polyclonal antibody, Biotin

Applications Immunocytochemical and Immunohistochemical staining for the detection of IgM at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates.
To demonstrate circulating IgM antibodies in serodiagnostic microbiology and autoimmune diseases.
To identify a specific antigen using an reference antibody of bovine origin known to be of the IgM isotype in the middle layer of the indirect test procedure.
In non-isotopic assay methodology (e.g. ELISA) to measure IgM in Bovine serum or other body fluids.
As a second step an avidin or streptavidin conjugate of the user’s choice has to be used.
Recommneded Dilutions:
Histochemistry and Cytochemistry: 1/50-1/250.
ELISA and comparable non-precipitating antibody-binding assays: 1/100-1/500.
Reactivities Bovine
Conjugation Biotin

Mouse IgM (Fc specific) rabbit polyclonal antibody, HRP

Applications ELISA.
Dot blot.
Immunoblotting.
Immunocytochemistry.
Immunohistochemistry on Paraffin Sections.
In enzyme-immunocytochemical and immunohistochemical staining for the detection of IgM at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates; to demonstrate circulating IgM antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen using a reference antibody of mouse origin known to be of the IgM isotype in the middle layer of the indirect test procedure; in non-isotopic assay methodology (e.g. ELISA) to measure IgM in mouse serum or other body fluids. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Recommended Working Dilutions
Histochemical and cytochemical Use: 1/100- 1/500.
ELISA and comparable non-precipitating antibody-binding assays: 1/1,000- 1/5,000.
Reactivities Mouse
Conjugation HRP

Mouse IgM (Fc specific) rabbit polyclonal antibody, Biotin

Applications In immunocytochemical and immunohistochemical staining of IgM at the cellular and subcellular level of appropriately treated cell and tissue substrates; to demonstrate circulating IgM antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen using a reference antibody of mouse origin known to be of the IgM isotype in the middle layer of the indirect test procedure; in non-isotopic assay methodology (e.g. ELISA) to measure IgM in mouse serum or other body fluids. As a second step an avidin or streptavidin conjugate of the user’s choice has to be used. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Recommended Working Dilutions:
Histochemical and cytochemical Use: 1/100- 1/2500.
ELISA and comparable non-precipitating antibody-binding assays: 1/1000- 1/5000.
Reactivities Mouse
Conjugation Biotin

Guinea pig IgG (H+L chain) rabbit polyclonal antibody, HRP

Applications Suitable for Immunoblotting (Western or Dot blot), ELISA, Immunoperoxidase electron microscopy and Immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency.
Note: This product has been assayed against 1.0 µg of Guinea Pig IgG in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:4,000 to 1:20,000 of the reconstitution concentration is suggested for this product.
Recommended Dilutions:
ELISA: 1/100,000.
Western Blot: 1/2,000-1/10,000.
Immunohistochemistry: 1/500-1/2,500.
Reactivities Guinea Pig
Conjugation HRP

Canine IgG (Fc specific) rabbit polyclonal antibody, Aff - Purified

Applications Suitable for Immunoprecipitation, Immunodiffusion, Conjugation and most immunological methods requiring lot-to-lot consistency, high titer and specificity.
ELISA: 1/20,000-1/100,000.
Western Blot: 1/2,000-1/10,000.
Immunohistochemistry: 1/1,000-1/5,000.
Reactivities Dog
Conjugation Unconjugated

Human IgA (alpha chain) rabbit polyclonal antibody, Aff - Purified

Applications Suitable for Immunoprecipitation, Immunodiffusion, conjugation and most immunological methods requiring lot-to-lot consistency, high titer and specificity.
Recommended dilutions:
ELISA: 1/5,000-1/20,000.
Western blot: 1/500-1/2,000.
IHC: 1/250-1:1,000
Reactivities Human
Conjugation Unconjugated

Chicken IgG / Chicken IgY (H+L chain) rabbit polyclonal antibody, Biotin

Applications Suitable for Immunoblotting, ELISA, Immunohistochemistry, Immunomicroscopy as well as other antibody based assays using streptavidin or avidin conjugates requiring lot-to-lot consistency.
Recommended Dilutions:
ELISA: 1/500,000.
Western blot: 1/2,000-1/20,000.
Immunohistochemistry: 1/1,000-1/5,000.
Reactivities Chicken
Conjugation Biotin

Mouse IgG (Fc specific) rabbit polyclonal antibody, Aff - Purified

Applications Immunoprecipitation.
Immunodiffusion.
Conjugation and most immunological methods requiring lot-to-lot consistency, high titer and specificity.
Recommended dilutions:
ELISA: 1/40,000-1/180,000.
Western blot: 1/2,000-1/10,000.
Immunohistochemistry: 1/1,000-1/5,000.
Reactivities Mouse
Conjugation Unconjugated

Rabbit Monoclonal Anti-Human Lambda Light Chain antibody, clone RM127

Applications ELISA: 0.05ug/mL-0.2ug/ml; ICC/IHC: 0.5ug/mL-2ug/mL
Reactivities Human lambda light chain
Conjugation Unconjugated

Rabbit Monoclonal Anti-Mouse IgM (mu-Chain) antibody, clone RM109

Applications ELISA: 0.005ug/mL – 0.2ug/mL, ICC, IHC: 0.5ug/mL-2ug/mL
Reactivities Mouse IgM mu-chain
Conjugation Unconjugated

CNY 8,025.00

4周

VhH Antibody

Applications WB: 1:1,000-1:5,000
ELISA: 1:10,000-1:50,000
Conjugation Unconjugated

CNY 2,119.00

4周

VhH Antibody

Applications WB: 1:1,000-1:5,000
ELISA: 1:10,000-1:50,000
Conjugation Unconjugated

Mouse lambda light chain rabbit polyclonal antibody, HRP

Applications ELISA: 1/10,000 - 1/50,000 .
Western Blot: 1/1,000 - 1/5,000.
Immunohistochemistry on paraffin sections: 1/500 - 1/2,500.
Reactivities Mouse
Conjugation HRP

Human IgA rabbit polyclonal antibody, Purified

Applications Immunohistochemistry on Frozen and Paraffin Sections (ABC method: 1/25-1/100).
Formalin-Fixed Paraffin Embedded Tissue Sections requires High temperature antigen unmasking with 10mM citrate buffer, pH 6.0 must be performed prior to the immunostaining.
Incubation Time: 30 minutes at room temperature.
Positive Control: Tonsil.
Reactivities Human
Conjugation Unconjugated