Human IgA rabbit polyclonal antibody, Purified
| Applications | Immunohistochemistry on Frozen and Paraffin Sections (ABC method: 1/25-1/100). Formalin-Fixed Paraffin Embedded Tissue Sections requires High temperature antigen unmasking with 10mM citrate buffer, pH 6.0 must be performed prior to the immunostaining. Incubation Time: 30 minutes at room temperature. Positive Control: Tonsil. |
| Reactivities | Human |
| Conjugation | Unconjugated |
Bovine IgG (H+L chain) rabbit polyclonal antibody, Biotin
| Applications | Immunoblotting (1/2,000-1/10,000). ELISA (1/20,000-1/100,000). Immunohistochemistry (1/1,000-1/5,000). Immunomicroscopy. as well as other antibody based assays using streptavidin or avidin conjugates requiring lot-to-lot consistency. This product has been assayed against 1.0 µg of Bovine IgG in a standard capture ELISA using Peroxidase Conjugated Streptavidin and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1/4,000-1/20,000 of the reconstitution concentration is suggested for this product. |
| Reactivities | Bovine |
| Conjugation | Biotin |
Human IgG (Fc specific) rabbit polyclonal antibody, FITC
| Applications | This Fluorescein Conjugated antibody is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms. Recommended Dilutions: FLISA: 1/10,000-1/50,000. IF MIscroscopy: 1/1000-1/5000. |
| Reactivities | Human |
| Conjugation | FITC |
Monkey IgG (H+L chain) rabbit polyclonal antibody, Azide Free
| Applications | Can be used as unlabelled primary or secondary reagent for indirect detection techniques, to prepare conjugates with markers of the user’s own choice, to prepare an insoluble immunoaffinity adsorbent or a solid phase antibody reagent by coupling to an artificial carrier and as catching or detection antibody in non-isotopic methodology and solid phase immunochemistry. When applied in any cytochemical or histochemical procedure or solids phase coupling technique, the optimum concentration of the IgG preparation should always be established by titration. Recommended working dilutions: Histochemistry: 1/50 and 1/250. ELISA and comparable non-precipitating antibody-binding assays: 1/500 - 1/5000. |
| Reactivities | Monkey |
| Conjugation | Unconjugated |
Duck IgG (H+L chain) rabbit polyclonal antibody, HRP
| Applications | ELISA. Dot blot. Immunoblotting. Immunocytochemistry. Immunohistochemistry on Paraffin Sections. In enzyme-immunocytochemical and immunohistochemical staining for the detection of duck IgG, antigen or antibody, of appropriately treated cell and tissue substrates at the cellular and subcellular level. In nonisotopic assay methodology (e.g. ELISA) to identify and measure a specific IgG in duck serum or other body fluid. In electron microscopy, since the complex between the conjugated antibody and the antigen also has electron-dense properties. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Recommended Working Dilutions: Histochemical and Cytochemical Use: 1/100-1/500. ELISA and comparable non-precipitating antibody-binding assays: 1/1,000-1/5,000. |
| Reactivities | Duck |
| Conjugation | HRP |
Swine IgG (H&L) Antibody Fluorescein Conjugated
| Applications | IF: 1:1,000 - 1:5,000 FC: 1:500 - 1:2,500 FLISA: 1:10,000 - 1:50,000 |
| Conjugation | FITC |
F(ab')2 Swine IgG (H&L) Antibody Fluorescein Conjugated
| Applications | IF: 1:1,000 - 1:5,000 FC: 1:500 - 1:2,500 FLISA: 1:10,000 - 1:50,000 |
| Conjugation | FITC |
F(ab')2 Dog IgG (H&L) Antibody Texas Red™ Conjugated
| Applications | IF: 1:500-1:2,500 FC: 1:500 - 1:2,500 FLISA: 1:10,000 - 1:50,000 |
| Conjugation | Texas Red |
F(ab')2 Guinea Pig IgG (H&L) Antibody Phycoerythrin conjugated
| Applications | IF: 1:100-1:250 FC: User Optimized |
| Conjugation | Phycoerythrin |
Fab Sheep IgG (H&L) Antibody Rhodamine Conjugated
| Applications | IF: 1:1,000 - 1:5,000 FC: 1:500 - 1:2,500 FLISA: 1:10,000 - 1:50,000 |
| Conjugation | TRITC |
Fab Chicken IgG (H&L) Antibody Fluorescein Conjugated
| Applications | IF: 1:1,000 - 1:5,000 FC: 1:500 - 1:2,500 FLISA: 1:10,000 - 1:50,000 |
| Conjugation | FITC |
Fab Human IgG (H&L) Antibody Rhodamine Conjugated
| Applications | IF: 1:1,000 - 1:5,000 FC: 1:500 - 1:2,500 FLISA: 1:10,000 - 1:50,000 |
| Conjugation | TRITC |
Monkey IgG (H&L) Antibody
| Applications | WB: 1:10,000 - 1:60,000 ELISA: 1:50,000 - 1:100,000 |
| Conjugation | Unconjugated |
F(ab')2 Bovine IgG (H&L) Antibody Fluorescein Conjugated
| Applications | IF: 1:1,000 - 1:5,000 FC: 1:500 - 1:2,500 FLISA: 1:10,000 - 1:50,000 |
| Conjugation | FITC |
Chicken IgG (H&L) Secondary Antibody Fluorescein Conjugated
| Applications | IF: 1:1,000 - 1:5,000 FC: 1:500 - 1:2,500 FLISA: 1:10,000 - 1:50,000 |
| Conjugation | FITC |
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