Antibodies

Human IgG (H+L chain) chicken polyclonal antibody, Aff - Purified

Applications ELISA.
Western Blot (1/1000).
Immunocytochemistry.
Immunohistochemistry (1/250).
Quality Control: Antibodies were tested for specificity using Double-Immunodiffusion assays in agarose.
Reactivities Human
Conjugation Unconjugated

Rabbit IgG (H+L chain) chicken polyclonal antibody, Aff - Purified

Applications ELISA.
Western Blot (1/1000).
Immunocytochemistry.
Immunohistochemistry (1/250).
Quality Control: Antibodies were tested for specificity using Double-Immunodiffusion assays in agarose.
Reactivities Rabbit
Conjugation Unconjugated

Goat IgG (H+L chain) chicken polyclonal antibody, HRP

Applications ELISA: a 1/75,000 dilution gave an O.D. of 1.0 in a 15 minute reaction using Tetramethylbenzidine as substrate.
Western Blot (1/5000).
Quality Control: Antibodies were tested for specificity using Double-Immunodiffusion assays in Agarose gels and ELISA analysis.
Reactivities Goat
Conjugation HRP

IDO1 sheep polyclonal antibody, Purified

Applications ELISA, FC, IF, IHC, WB
Reactivities Human

Synphilin 1 (SNCAIP) rabbit polyclonal antibody, Purified

Applications ELISA, WB
Reactivities Human, Mouse

Rabbit IgG (H+L chain) chicken polyclonal antibody, HRP

Applications ELISA: a 1/20,000 dilution gave an O.D. of 1.0 in a 15 minute reaction using Tetramethylbenzidine as substrate.
Western Blot (1/5000).
Quality Control: Antibodies were tested for specificity using Double-Immunodiffusion assays in agarose gels and ELISA analysis.
Reactivities Rabbit
Conjugation HRP

Mouse IgG (H+L chain) chicken polyclonal antibody, HRP

Applications ELISA: a 1/70,000 dilution gave an O.D. of 1.0 in a 15 minute reaction using Tetramethylbenzidine as substrate.
Western Blot: 1/200 -1/5000.
Immunocytochemistry.
Immunohistochemistry: 1/250 - 1/5000.
Quality Control: Antibodies were tested for specificity using Double-Immunodiffusion assays in agarose gels and ELISA analysis.
Reactivities Mouse
Conjugation HRP

Chicken IgG / Chicken IgY (H+L chain) goat polyclonal antibody, Biotin

Applications ELISA.
Western Blot (1/5000).
Quality Control: Antibodies were analyzed using Immunoelectrophoresis and a Sandwich ELISA using HRP-labeled streptavidin. A 1/500,000 dilution was found to generate an O.D of 1.0 in a 30 minute reaction with Tetramethylbenzidine as the substrate.
Reactivities Chicken
Conjugation Biotin

Chicken IgG / Chicken IgY (H+L chain) goat polyclonal antibody, AP

Applications ELISA: In Sandwich ELISA a 1/800 dilution of GGHL-30PH-2 was found to generate an O.D of 1.0 in a 20 minute reaction with p-Nitrophenyl Phosphate as the substrate.
Western Blot (1/1000).
Immunoelectrophoresis: Chicken serum (3 µl) was placed in the center well (at the clear circle) and then subjected to electrophoresis. After separation, Goat anti-Chicken serum (75 μl) was placed in the lower trough and GGHL-30 (75 μl) (pre-conjugated affinity-purified antibody) was placed in the upper trough. After overnight incubation at 4°C, the gel was washed, fixed and stained with Coomassie. Note the single precipitin line between the center well and upper trough.
Quality Control: Antibodies were analyzed using Immunoelectrophoresis and a Sandwich ELISA.
Reactivities Chicken
Conjugation AP