Human IgM rabbit polyclonal antibody, Purified
| Applications | Immunohistochemistry with normal and neoplastic tissues (formalin fixed paraffin-embedded): the antibody may be diluted 1:150-1:250 in an ABC method, incubation time 30 minutes. Positive control: Tonsil. Cellular localization: Cytoplasmic. |
| Reactivities | Human |
| Conjugation | Unconjugated |
Human IgA mouse monoclonal antibody, clone DA43D3, Purified
| Applications | Suitable for Immunohistochemistry on Frozen (1 µg/ml, 1/400) and Paraffin Embedded Sections. Proteinase K pretreatment for antigen retrieval is recommended. Suggested Positive Control: Human tonsil. |
| Reactivities | Human |
| Conjugation | Unconjugated |
Human IgM mouse monoclonal antibody, clone DM3A3, Purified
| Applications | Immunohistochemistry on frozen sections: 1-2 µg/ml (1:200 - 1:400). Immunohistochemistry on paraffin sections: Proteinase K pretreatment for antigen retrieval is recommended. Suggested positive control: Human tonsil. |
| Reactivities | Human |
| Conjugation | Unconjugated |
Human IgG4 mouse monoclonal antibody, clone MRQ-44, Purified
| Applications | Immunohistochemistry on Frozen and Formalin-Fixed, Paraffin-Embedded Sections: 1/100-1/500. Preparation and Pretreatment: 1. Cut 3-4 μm section of formalin-fixed paraffin-embedded tissue and place on positively charged slides; dry overnight at 58°C. 2. Deparaffinize, rehydrate, and epitope retrieve; the preferred method is the use of Heat Induced Epitope Retrieval (HIER) techniques in conjunction with a pressure cooker. The preferred method allows for simultaneous deparaffinization, rehydration, and epitope retrieval. Upon completion, rinse with 5 changes of distilled or deionized water. 3. If using HRP detection system, place slides in peroxide block for 10 minutes; rinse. If using AP detection system, omit this step. Positive Control: Tonsil. |
| Reactivities | Human |
| Conjugation | Unconjugated |
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