Antibodies

Mouse IgA+IgG+IgM (H+L chain) goat polyclonal antibody, AP

Applications ELISA: 1/2,000-1/4,000.
Immunoblotting.
Immunohistochemistry.
Reactivities Mouse
Conjugation AP

Human IgG3 (Hinge Region) mouse monoclonal antibody, clone HP6050, AP

Applications ELISA: 1/500-1/1,000.
Western blotting.
Dot- and Slot-Immunoblotting
Immunohistochemistry on Frozen Sections.
Reactivities Human
Conjugation AP

Mouse IgG (Fc specific) goat polyclonal antibody, Ig Fraction

Applications ELISA: 1/20000-1/100000.
Western Blot: 1/2000-1/10000.
Immunohistochemistry: 1/1000-1/5,000.
Reactivities Mouse
Conjugation Unconjugated

Human IgG (F(ab)2 specific) goat polyclonal antibody, FITC

Applications Direct staining of fixed cell and tissue substrates, to demonstrate the intracellular presence of free of Ig
bound subunits of both kappa and lambda type. The absence of the Fc domain in the conjugate ensures minimal interaction with the tissue components and cell surfaces other than the primary antibody activity.
This conjugate is primarily intended for use in cell surface membrane staining procedures, to identify and quantitate Ig on B cells, especially if interference by Fc activity is expected.
Recommended Dilutions: 1/20-1/80.
This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Reactivities Human
Conjugation FITC

Rat IgG2a goat polyclonal antibody, HRP

Applications ELISA: 1/10,000.
Western Blot: 1/1,000-1/10,000.
Reactivities Rat
Conjugation HRP

Human IgG3 (F(ab)2 specific) mouse monoclonal antibody, clone NI 330 (HP 6050), Ascites

Applications To identify the presence of IgG3 in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as ELISA, indirect immunoperoxidase and indirect immunofluorescence staining of cytoplasmic IgG3, and immunoblotting.
General Recommended Dilutions:
Histochemical Use: 1/50-1/200.
ELISA: from 1/500 upwards.
Western blot: from 1/1000 upwards.
Reactivities Human
Conjugation Unconjugated

Human IgG1 (Fc specific) mouse monoclonal antibody, clone NI 132 (HP 6186), Ascites

Applications To identify the presence of IgG1 in Human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as ELISA, Indirect Immunoperoxidase and indirect Immunofluorescence staining of cytoplasmic IgG1, and Immunoblotting.
General Recommended Dilutions:
Histochemical Use: 1/50-1/200.
ELISA: from 1/500 upwards.
Western blot: from 1/1000 upwards.
Reactivities Human
Conjugation Unconjugated

Mouse IgG goat polyclonal antibody, AP, Aff - Purified

Applications ELISA: 1/10000.
Western Blot: 1/5000-1/10000,
Immunohistochemistry.
Reactivities Mouse
Conjugation AP

Biotin Conjugated Rabbit Monoclonal Anti-Human Ig Light Chains antibody 3, clone RM129

Applications ELISA: 0.02ug/mL – 0.25ug/mL; IHC, ICC: 0.5ug/mL – 2ug/mL
Reactivities Human Ig light chain
Conjugation Biotin

Rabbit Monoclonal Anti-Human IgA1 antibody, clone RM124

Applications ELISA: 50ng/well – 200ng/well (for Capture); 0.05ug/mL – 0.2ug/mL (for Detection); IHC, ICC: 0.5ug/mL – 2ug/mL
Reactivities Human IgA1
Conjugation Unconjugated

Rabbit IgG (Fc specific) goat polyclonal antibody, HRP

Applications Suitable for Immunoblotting (Western or Dot blot), ELISA, Immunoperoxidase electron microscopy and Immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency.
Recommended Dilutions:
ELISA: 1/110,000.
Western Blot: 1/2,000-1/20,000.
Immunohistochemistry: 1/1,000-1/5,000.
Reactivities Rabbit
Conjugation HRP

Mouse IgG1 (gamma 1 chain specific) rabbit polyclonal antibody, HRP

Applications Anti Mouse IgG1 secondary antibody conjugate is suitable for ELISA, Immunohistochemistry, Western blotting as well as other anti IgG1 antibody based assays.
Recommended Dilutions:
ELISA: 1/300,000.
Western Blot: 1/2,000-1/10,000.
Immunohistochemistry: 1/500-1/2,500.
Reactivities Mouse
Conjugation HRP

Rabbit IgG (heavy chain) rat monoclonal antibody, clone RG-1, Aff - Purified

Applications ELISA.
Immunofluorescence.
Reactivities Rabbit
Conjugation Unconjugated

Human IgG (heavy chain) mouse monoclonal antibody, clone B33/20, Purified

Applications ELISA.
Reactivities Human
Conjugation Unconjugated

Human IgA + IgG + IgM (adsorbed F(ab)2 Fragment) goat polyclonal antibody, HRP

Applications Suitable for Immunoblotting (Western: 1/1,000-1/10,000 or dot blot), ELISA (1/10,000-1/50,000), immunoperoxidase electron microscopy and Immunohistochemistry (1/500-1/2,500) as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency.
This product has been assayed against 1.0 µg of Human IgG in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1/2,000 to 1/8,000 of the reconstitution concentration is suggested for this product.
Reactivities Human
Conjugation HRP