Antibodies

Rat IgE (Fc specific) goat polyclonal antibody, HRP

Applications This antibody can be used
In Immunocytochemical and Immunohistochemical staining for the detection of IgE at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates, and to demonstrate circulating antibodies in serodiagnostic microbiology.
In non-isotopic assay methodology (e.g. ELISA) to identify and measure IgE in rat serum or other body fluid.
As a second step an avidin or streptavidin conjugate of the user’s choice has to be used.
Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Recommended Dilutions:
Histochemistry and Cytochemistry: 1/50-1/250.
ELISA and comparable non-precipitating antibody-binding assays: 1/1,000-1/5,000.
Reactivities Rat
Conjugation HRP

Mouse IgE (Fc specific) rabbit polyclonal antibody, HRP

Applications Can be used in enzyme-immunocytochemical and immunohistochemical staining for the detection of IgE at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates; to demonstrate circulating IgE antibodies in mouse serum or other body fluids; in non-isotopic assay methodology (e.g. ELISA) to identify and measure IgE in mouse serum or other body fluids. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Recommended working dilutions:
Histochemical and Cytochemical: 1/100 - 1/500.
ELISA and comparable non-precipitating antibody-binding assays: 1/500 - 1/5000.
Reactivities Mouse
Conjugation HRP

Rat IgG (H+L chain) rabbit polyclonal antibody, HRP

Applications Suitable for Immunoblotting (Western or Dot blot), ELISA, Immunoperoxidase electron microscopy and Immunohistochemistry as well as other peroxidas antibody based enzymatic assays requiring lot-to-lot consistency.
Recommended Dilutions:
ELISA: 1/170,000
Western blot: 1/1,000-1/5,000.
Immunohistochemistry: 1/500- 1/2,500.
Reactivities Rat
Conjugation HRP

Dog IgG F(c) Antibody Peroxidase Conjugated

Applications WB: 1:1,000 - 1:10,000
IHC: 1:500 - 1:2,500
ELISA: 1:10,000 - 1:50,000
Conjugation HRP

Horse IgG (H&L) Antibody Peroxidase Conjugated

Applications WB: 1:2,000 - 1:20,000
IHC: 1:1,000 - 1:5,000
ELISA: 1:20,000 - 1:60,000
Conjugation HRP

Human IgG F(c) Antibody Peroxidase Conjugated Pre-Adsorbed

Applications WB: 1:1,000 - 1:10,000
IHC: 1:500 - 1:2,500
ELISA: 1:200,000
Conjugation HRP

Goat IgG (H&L) Antibody Peroxidase Conjugated

Applications WB: 1:1,000 - 1:5,000
IHC: 1:500 - 1:2,500
ELISA: 1:10,000 - 1:150,000
Reactivities Goat
Conjugation HRP

Guinea Pig IgG F(c) Antibody Peroxidase Conjugated

Applications WB: 1:1,000 - 1:10,000
IHC: 1:500 - 1:2,500
ELISA: 1:10,000 - 1:50,000
Conjugation HRP

Chicken IgG (H&L) Antibody Peroxidase Conjugated

Applications WB: 1:2,500 - 1:10,000
IHC: 1:1,000 - 1:5,000
ELISA: 1:25,000 - 1:75,000
Conjugation HRP

Chicken IgG (H&L) Antibody Peroxidase Conjugated Pre-Adsorbed

Applications WB: 1:1,000 - 1:5,000
IHC: 1:500 - 1:2,500
ELISA: 1:65,000
Conjugation HRP

Dog IgG (H&L) Antibody Peroxidase Conjugated

Applications WB: 1:1,000 - 1:10,000
IHC: 1:500 - 1:2,500
ELISA: 1:50,000 - 1:150,000
Conjugation HRP

Rat IgG (H&L) Antibody Peroxidase Conjugated

Applications WB: 1:1,000 - 1:10,000
IHC: 1:500 - 1:2,500
ELISA: 1:10,000 - 1:50,000
Conjugation HRP

Rabbit IgG (H&L) Antibody Peroxidase Conjugated Pre-Adsorbed

Applications WB: 1:5,000-1:40,000
IHC: 1:500-1:5,000
ELISA: 1:10,000-1:100,000
Reactivities Rabbit
Conjugation HRP

Rabbit IgG F(c) Antibody Peroxidase Conjugated

Applications WB: 1:1,000 - 1:10,000
IHC: 1:500 - 1:2,500
ELISA: 1:10,000 - 1:50,000
Conjugation HRP

Rat IgG F(c) Antibody Peroxidase Conjugated

Applications WB: 1:2,000 - 1:10,000
IHC: 1:1,000 - 1:5,000
ELISA: 1:130,000
Conjugation HRP