Oligo annealing buffer (10X)
CRISPR Accessories
Cre recombinase mammalian expression vector
PCR Primer mix to confirm AAVS1 integration, amplifying the left integration junction, 2 nmols
Cas9 in vitro digestion buffer, 10x
CRISPR/Cas9 starter kit (HA tagging human HSP60 at C-terminus)