Recombinant Proteins

Proteins

Recombinant Proteins

Human

POU5F1

BDNF

BMP6

BMP7

BMP8B

CD14

CD34

FGF10

GDF5

HBZ

KLF4

NANOG

SMAD2

SMAD3

SOX2

E. coli

C-Myc/DDK

N-His

His-tag

Tag Free

C-His

Myc-DDK

C-6His

C-Fc

N-GST and C-His

C-DDK/His

C-Human Fc

C-hFc

His-Cam-tag

N-GST and C-HIS

N-His&Flag

N-T7

Druggable Genome

Transmembrane

Secreted Protein

Transcription Factors

Protease

Stem cell - Pluripotency

ES Cell Differentiation/IPS

Protein Kinase

Phosphatase

Adult stem cells

Cancer stem cells

Embryonic stem cells

Induced pluripotent stem cells

Ion Channels: Other

Stem cell relevant signaling - TGFb/BMP signaling pathway

Stem cell relevant signaling - Wnt Signaling pathway

Stem cell relevant signaling - JAK/STAT signaling pathway

Ion Channels: Cys-loop Receptors

Nuclear Hormone Receptor

Ion Channels: Sodium

P450

Stem cell relevant signaling - DSL/Notch pathway

0.01 units Xanthine oxidase.
2. Equilibrate to 25°C and monitor at A550nm until the value is constant using a spectrophotometer.
3. Add 20 µl of recombinant SOD2 protein to 50 µg/ml in assay buffer. 4. Mix by inversion and record the increase at A550nm for 5 minutes.

0.033% (w/v) bovine serum albumin.
2. Equilibrate to 37°C and monitor at A340nm until the value is constant using a spectrophotometer
3. Add 50ul of recombinant LDHA protein in various concentrations (0.1ug

0.12 mM beta-nicotinamide adenine dinucleotide

0.5ug) in assay buffer.
4. Mix by inversion and record the increase at A340nm for 5 minutes.

0.9 mM Xanthine

10mM pNPP.
2. Pre-warm reaction mix in heat block at 37°C.
3. Add recombinant PPP1CA solution with various concentrations (2ug

1mM MnCl2

2.3 mM Pyruvate

2mM DTT

3ug) in 200ul reaction buffer.
4. Mix by inversion and incubate at 37°C for 10 minutes.
5. Stop the reaction by adding the 800ul of 0.1N NaOH.
6. Record the absorbance at 405nm.

5.5 mM Ethylendiaminetetraacetic acid

50mM NaCl

66 mM Cytochrom-C

Citric acid 0.055 g

D - glucose 2.05g in DW100 ml)
2. Centrifuge at 15000 rpm for 10 minutes and wash four times with PBS.
3. Dilute packed cells in a 0.5 mg/ml trypsin - EDTA solution to give 4% red cell suspension.
4. Incubate for 1h at 37°C and wash four times with PBS.
5 . Dilute packed cells in PBS to give 4% red cell suspension.
6 . Load 50ul of 0.5% BSA - in - 0.15M - NaCl solution and 25ul of 4% Red Cell - in - PBS in u shaped wells.
7 . Add 25ul of serial diluted galectin protein in PBS to each well plate . (Round bottom 96 well plate)
8 . Incubate for 30min at room temperature to observe visible agglutination.

Protocol: Activity Assay
1. Prepare a 180 µl assay buffer into a suitable container and pre-chill on ice before use: The concentrations are 54 mM Potassium Phosphate

Protocol: Activity Assay
1. Prepare a 1.5 ml reaction mix into a suitable container

Protocol: Activity Assay
1. Prepare a 200 ul reaction mix into a suitable container : The final concentrations are 10mM Tris (pH 7.5)

Protocol: Activity Assay
1. Prepare 170 ul assay buffer into a suitable container and pre-chill on ice before use:
The final concentrations are 200 mM Tris-HCl

Protocol: Biological Assay :
1. Mix equal volumes of human blood and Alsever’s solution (pH 7.0). (Alsever’s solution: NaCl 0.42 g

Sodium citric aci d 0.8g

adjust to pH 7.5 at 37°C and pre-chill on ice before use: The final concentrations are 100 mM Sodium phosphate

and 20nM chymotrypsin.
2. Add 10 ul of recombinant Cyclophilin A protein with 1 ug in assay buffer.
3. Mix by inversion and equilibrate to 1°C and monitor the A405nm until the value is constant using a spectrophotometer.
4. Add 20 ul pre-chilled 5mM suc-AAFP-pNA. (Substrate was dissolved in TFE that contained 460mM LiCl to a concentration of 3 mM)
5. Record the increase in A405 nm for 30 minutes at 25°C.

pH 8.0

reduced form

TGF-beta signaling pathway

Hedgehog signaling pathway

MAPK signaling pathway

Pathways in cancer

Adherens junction

Cell cycle

Colorectal cancer

Cytokine-cytokine receptor interaction

Hematopoietic cell lineage

Pancreatic cancer

Regulation of actin cytoskeleton

Wnt signaling pathway

Cell adhesion molecules (CAMs)

Chronic myeloid leukemia

Huntington's disease

Melanoma

Neurotrophin signaling pathway

Pathogenic Escherichia coli infection

Toll-like receptor signaling pathway

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