Proteins

Proteins

Native Proteins

Druggable Genome

WB

Transmembrane

Transcription Factors

Secreted Protein

Protein Kinase

ES Cell Differentiation/IPS

Protease

Stem cell - Pluripotency

Phosphatase

GPCR

Ion Channels: Other

Adult stem cells

Embryonic stem cells

Nuclear Hormone Receptor

Cancer stem cells

Induced pluripotent stem cells

P450

Ion Channels: Potassium

Stem cell relevant signaling - JAK/STAT signaling pathway

Stem cell relevant signaling - TGFb/BMP signaling pathway

Stem cell relevant signaling - Wnt Signaling pathway

Stem cell relevant signaling - DSL/Notch pathway

Ion Channels: Cys-loop Receptors

Ion Channels: Transient receptor potential

Ion Channels: Glutamate Receptors

Ion Channels: ATP Receptors

Ion Channels: Sodium

Ion Channels: Cyclic nucleotide gated

Suitable for use in ELISA and Western blots.

Ion Channels: Calcium

Suitable for use in ELISA and Western blot.

Suitable for ELISA and Western blot.

ELISA.
Western Blot.

Serological studies of influenza A virus

Suitable for use in ELISA

Suitable for use in ELISA.

immunogen for antibody production.

immunogen for antibody production.
Has been tested with MAb to Influenza A in ELISA.

ELISA

ELISA.
Western Blot.

Serological studies of Influenza B virus

000.

ELISA.
Lateral Flow.

ELISA.

ELISA.
Western Blot.

Colloidal Gold and Latex Beads.

ELISA and Western blot.

ELISA.

ELISA.
Western blot.
Lateral flow.

ELISA: 0.5-1.0 µg/ml coating concentration.
After thawing

Immunogen

Recommended for use in standard solid phase immunoassays.

Serological studies of Influenza A virus

Serological studies of influenza B virus

Suitable in ELISA

Suitable in Western Blot with a suggested dilution of 1:1

Use in standard solid phase immunoassays.

Western blot

Western blot and Immunochromatographic assays.

Western blot and immunochromatographic assays.

mix thoroughly by vortexing before use.

000 and 30

000 and 50

000 daltons and a doublet band at approximately 28

000 daltons that are not glycosylated.

000 or less. This should be performed in a cold room.

000) and Immunohistochemistry (1/200-1/1

000) as well as other phosphatase-Biotin/biotin based enzymatic assays.
Assay by Immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine) and anti-Biotin.

000-1/32

1/500-1/2

10 mM Tris-HCl

2 mM dithiothreitol

500)

ELISA (0.5 µg/ml).
Western blot.

ELISA and Western blot.
Using an antigen Capture ELISA

ELISA.
Western blot.

ELISA.
Western Blot.
Lateral Flow.

ELISA.
Suitable for use as a Control or standard in indirect trapping ELISA for quantitation of antigen in serum using a standard curve.
Immunoprecipitation.
Western blotting.

ELISA.
Western blot: 1/1000.
Colloidal Gold and Latex Beads.

ELISA: this protein is suitable for use as a Mouse IgE standard in ELISA assays.

Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoassays.
Immunization.
Protocol: Reconstitution to filaments is performed by mixing equimolar amounts of keratins of type I and type II at concentrations of approx. 0.5 mg/ml

RIA.
Western blot.

Antigen in ELISA and Western blots.

Apo AI can be renatured into PBS

Colloidal Gold and Western Blot.
Not recommended for Latex Beads.

ELISA (1/8

ELISA and Western blot Immunoassay

ELISA and Western blot.
Glycosylation is heterogeneous and results in several immunoreactive products on Western blots that range from approximately 40

ELISA.
Western blot.

ELISA: 0.4-0.8 µg/well.
After thawing

FACS

Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoassays.
Immunization.

Second order rate constants for inhibition of:
uPA = 6.3 x 10e6 M-1s-1
tPA = 5.7 x 10e6 M-1s-1

Should be sonicated immediately prior to use to ensure that the preparation is uniform. Used for both IgG and IgM detection in assays which include EIA with polystyrene and latex solid phases and M capture formats.

Specific methodologies have not been tested using this product.

Suitable for ELISA

Suitable for ELISA.

Suitable for Immunoblotting (Western or Dot blot

Suitable for use as a positive control in Lateral Flow assays and when spiked into stool samples.

Suitable for use in EIA and Western Blot. Each laboratory should determine an optimum working titer for
use in its particular application.

Suitable for use in ELISA and Western blot

Suitable for use in ELISA and Western blot immunoassay.

Suitable for use in ELISA and other phase Immunoassays.
BIN008 is typically required for IgM antibody testing.

Suitable for use in Western blot.

Suitable in ELISA.

Suitable to promote the adhesion and propagation of cells in vitro when used to coat cell culture surfaces

TBS and other common buffers by dialysis using a membrane with a nominal molecular weight cut-off of 14

Tested as solid-phase coating antigen in ELISA with anti-HBsAg antibodies.

Tested in ELISA with anti-HBsAg antibodies.

The DYKDDDDK peptide (Flag) is used as a fusion tag for immunoaffinity chromatography.

The antigen preparation should be sonicated immediately prior to use to ensure that the preparation is uniform. Grade 2 antigen is suitable for use for both IgG and IgM detection in assays which include EIA with polystyrene and latex solid phases. This preparation may be further purified to meet the needs of a particular assay format.

This preparation may be used as is in a variety of immunoassay formats or may be further purified to meet the needs of a particular assay format. May be used for both IgG and IgM detection in assays which include EIA with polystyrene and latex solid phases.

Variety of Immunoassay formats including Microwell ELISA.
This product should be sonicated immediately prior to use to ensure that the preparation is uniform.

Western Blot and ELISA.

both dissolved in 9.5 M urea buffer (see above). Protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4 M urea and then to low salt condition (50 mM NaCl

calibrator in immunoassays and in vitro studies.

calibrator in immunoassays and in vitro studies.

completely thaw the tube at 37°C without agitation

e.g. Dulbecco's PBS).

each lot is tested against a reference material for both IGM and IgG activity.

flicking or mixing

glassware and microcarrier beads. After spin and opening of the vial

immunogen for antibody production. Has been tested with anti-influenza A monoclonal antibodies in ELISA.

immunogen for antibody production. Has been tested with anti-influenza B monoclonal antibodies in ELISA.

immunogen for antibody production. Has been tested with anti-influenza B monoclonal antibodies in ELISA.

including plasticware

mix thoroughly by vortexing before use!

pH 7.4).
For immunization purposes

the solution can be further dialyzed against PBS (phosphate buffered saline

then gently mix the contents with a pipette.

Cell culture

2

Yes