pCas-Guide-Puro-CRISPRi Vector, All-in-one Vector for CRISPR/Cas9 Gene Interference with Puro selection marker, dCas9-KRAB-MeCP2 and gRNA cloning site.
Vectors
pT7-Cas9-Nickase vector (Cas9 D10A) (10 µg)
Function | Cas9 Nickase |
pLenti-Cas9 vector, Cas9 in lenti vector (10 ug)
Function | Cas9 only vectors |
p-xCas9-Guide vector (with xCas9 expression) for genomic target sequence cloning (10 µg)
Function | All-in-One CRISPR/Cas9 vectors |
Donor vector with AAVS1 homologous arms, C-terminal mGFP tag, Blasticidin resistance, ready for transgene cloning
Function | AAVS1 donor vector |
pCas-Guide-Puro-CRISPRi-Scramble, scramble gRNA control in GE100083 vector (with Puro selection)
pLenti-Cas9-Myc-DDK-P2A-Puro control plasmid containing the expression cassette for Cas9-Myc-DDK fusion protein and Puromycin resistance.
Function | Cas9 only vectors |
Validated sgRNA targeting RFP dead mutant in GE100041C cells