pCas-Guide-CRISPRa-Scramble, scramble gRNA control in GE100055 vector (no selection marker/reporter)
Function | CRISPRa scramble control vector |
pCas-Guide-CRISPRa-Scramble, scramble gRNA control in GE100055 vector (no selection marker/reporter)
Function | CRISPRa scramble control vector |
pLenti-Cas9-mGFP-P2A-Puro control plasmid containing the expression cassette for Cas9-mGFP fusion protein and Puromycin resistance.
Function | Cas9 only vectors |
HuSH shRNA RFP Lenti Cloning Vector (pRFP-C-shLenti Vector), 5 ug
Viral Packaging | Lentiviral |
pCas-Guide-Puro-CRISPRi-Scramble, scramble gRNA control in GE100083 vector (with Puro selection)
pCas-Guide-Puro-CRISPRa-Scramble, scramble gRNA control in GE100081 vector (with Puro selection)
pCas-Guide-Nickase vector for gRNA cloning (with Cas9 H840A expression) (10 µg)
Function | Cas9 Nickase |
pLenti-C-Myc-DDK-P2A-Puro-mGFP, Lenti vector with C-terminal Myc-DDK tag, P2A-Puro selection marker and mGFP reporter gene
C-Tag | Myc-DDK |
E. coli Selection | Chloramphenicol (34 ug/mL) |
Cell Selection | Puromycin |
Catalytically inactive Cas9 (D10A/H840A) vector for gRNA cloning (10 µg)
Function | Cas9 Nickase |
pLenti-Cas9-Myc-DDK-P2A-Puro control plasmid containing the expression cassette for Cas9-Myc-DDK fusion protein and Puromycin resistance.
Function | Cas9 only vectors |
29-mer Scrambled shRNA Cassette in p-RFP-C-shLenti Vector, 5 ug
Viral Packaging | Lentiviral |
29-mer Scrambled shRNA Cassette in pGFP-A-shAAV Vector, 5ug
Viral Packaging | Adeno-associated virus (AAV) |
Validated sgRNA targeting RFP dead mutant in GE100041C cells
29-mer Scrambled shRNA Cassette in pB-RS Vector, 5 ug
Viral Packaging | Retroviral |