R-Phycoerythrin goat polyclonal antibody, AP
Applications | ELISA, IHC, WB |
R-Phycoerythrin goat polyclonal antibody, AP
Applications | ELISA, IHC, WB |
Chicken IgG / Chicken IgY (H+L chain) goat polyclonal antibody, AP
Applications | ELISA: In Sandwich ELISA a 1/800 dilution of GGHL-30PH-2 was found to generate an O.D of 1.0 in a 20 minute reaction with p-Nitrophenyl Phosphate as the substrate. Western Blot (1/1000). Immunoelectrophoresis: Chicken serum (3 µl) was placed in the center well (at the clear circle) and then subjected to electrophoresis. After separation, Goat anti-Chicken serum (75 μl) was placed in the lower trough and GGHL-30 (75 μl) (pre-conjugated affinity-purified antibody) was placed in the upper trough. After overnight incubation at 4°C, the gel was washed, fixed and stained with Coomassie. Note the single precipitin line between the center well and upper trough. Quality Control: Antibodies were analyzed using Immunoelectrophoresis and a Sandwich ELISA. |
Reactivities | Chicken |
Conjugation | AP |
Mouse IgG (adsorbed) goat polyclonal antibody, AP
Applications | ELISA: 1/200-1/2500. Western Blot: 1/100-1/500. |
Reactivities | Mouse |
Conjugation | AP |
Bovine IgM sheep polyclonal antibody, AP
Applications | ELISA: 1/1000-1/10000. Western Blot: 1/5000-1/50000. |
Reactivities | Bovine |
Conjugation | AP |
Bovine IgG sheep polyclonal antibody, AP
Applications | ELISA: 1/5000-1/50000. |
Reactivities | Bovine |
Conjugation | AP |
Mouse IgG (Fc specific), F(ab)2 Fragment, adsorbed goat polyclonal antibody, AP
Applications | Suitable for Immunoblotting (Western or dot blot), ELISA and immunohistochemistry as well as other phosphatase-antibody based enzymatic assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity. This product has been assayed against 1.0 µg of Mouse IgG in a standard capture ELISA using pNPP p-nitrophenyl phosphate as a substrate for 30 minutes at room temperature. A working dilution of 1/1,000 to 1/5,000 of the reconstitution concentration is suggested for this product. Recommended Dilutions: ELISA: 1/2000-1/10000. Western Blot: 1/500-1/2500. Immunochemistry: 1/200-1/1000. |
Reactivities | Mouse |
Conjugation | AP |