Products

Antibodies

cDNA Clones

Proteins

Recombinant Proteins

Over-expression Lysates

CytoSections

Mass Spec Standards

Human

CD44

ONCOSTATIN M

STAT6

CCN4

CYCLIN D1

DLL1

HES1

GLI1

HDAC1

STAT5A

CYCLIN E1

NOTCH1

HOXB4

DLL3

OSM

DLL4

SMOOTHENED

IHC

WB

HEK293T

E. coli

HEK293

Sf9

C-Myc/DDK

C-MYC/DDK

C-His

N-His

C-Fc

His-tag

Tag Free

C-DDK

C-hFc

Myc-DDK

N-GST and C-His

MVPro

SDS PAGE

Druggable Genome

WB

Transmembrane

Transcription Factors

Secreted Protein

Protein Kinase

ES Cell Differentiation/IPS

Protease

Stem cell - Pluripotency

Phosphatase

GPCR

Ion Channels: Other

Adult stem cells

Embryonic stem cells

Nuclear Hormone Receptor

Cancer stem cells

Induced pluripotent stem cells

P450

Ion Channels: Potassium

Stem cell relevant signaling - JAK/STAT signaling pathway

Stem cell relevant signaling - TGFb/BMP signaling pathway

Stem cell relevant signaling - Wnt Signaling pathway

Stem cell relevant signaling - DSL/Notch pathway

Ion Channels: Cys-loop Receptors

Ion Channels: Transient receptor potential

Ion Channels: Glutamate Receptors

Ion Channels: ATP Receptors

Ion Channels: Sodium

Ion Channels: Cyclic nucleotide gated

Suitable for use in ELISA and Western blots.

Ion Channels: Calcium

Suitable for use in ELISA and Western blot.

Suitable for ELISA and Western blot.

ELISA.
Western Blot.

Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoassays.
Immunization.

Serological studies of influenza A virus

Suitable for use in ELISA

Suitable for use in ELISA.

immunogen for antibody production.

immunogen for antibody production.
Has been tested with MAb to Influenza A in ELISA.

ELISA.

ELISA

ELISA.
Western Blot.

FACS

Serological studies of Influenza B virus

000.

ELISA.
Lateral Flow.

ELISA.
Western Blot.

Colloidal Gold and Latex Beads.

ELISA and Western blot.

ELISA.

ELISA.
Western blot.
Lateral flow.

ELISA: 0.5-1.0 µg/ml coating concentration.
After thawing

Immunogen

Recommended for use in standard solid phase immunoassays.

Serological studies of Influenza A virus

Serological studies of influenza B virus

Suitable in ELISA

Suitable in Western Blot with a suggested dilution of 1:1

Use in standard solid phase immunoassays.

Western blot

Western blot and Immunochromatographic assays.

Western blot and immunochromatographic assays.

mix thoroughly by vortexing before use.

0.01 units Xanthine oxidase.
2. Equilibrate to 25°C and monitor at A550nm until the value is constant using a spectrophotometer.
3. Add 20 µl of recombinant SOD2 protein to 50 µg/ml in assay buffer. 4. Mix by inversion and record the increase at A550nm for 5 minutes.

0.033% (w/v) bovine serum albumin.
2. Equilibrate to 37°C and monitor at A340nm until the value is constant using a spectrophotometer
3. Add 50ul of recombinant LDHA protein in various concentrations (0.1ug

0.1 mg/ml Ovalbumin

0.1% (w/v) insulin

0.12 mM beta-nicotinamide adenine dinucleotide

0.5 mM EDTA

0.5ug) in assay buffer.
4. Mix by inversion and record the increase at A340nm for 5 minutes.

0.9 mM Xanthine

0.9ng) in assay buffer and equilibrate to 37C for 10 minutes. (Assay buffer: 50mM Tris-HCl

000 and 30

000 and 50

000 daltons and a doublet band at approximately 28

000 daltons that are not glycosylated.

000 or dot blot)

000 or less. This should be performed in a cold room.

000)

000) and Immunohistochemistry (1/200-1/1

000) as well as other Glucose Oxidase-biotin-avidin based enzymatic assays.

000) as well as other phosphatase-Biotin/biotin based enzymatic assays.
Assay by Immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine) and anti-Biotin.

000-1/200

000-1/32

000-1/40

000-1/5

1 mM DTT

1/500-1/2

10 mM Tris-HCl

10mM pNPP.
2. Pre-warm reaction mix in heat block at 37°C.
3. Add recombinant PPP1CA solution with various concentrations (2ug

1mM DTT

1mM MnCl2

2 mM dithiothreitol

2.3 mM Pyruvate

2mM DTT

2mM EDTA.
2. Add 4ug of recombinant thioredoxin into reaction cocktail and mix immediately.
3. Incubate at 25°C for 20 minutes.
4. Record the increase in A650nm for 15 minutes

3ug) in 200ul reaction buffer.
4. Mix by inversion and incubate at 37°C for 10 minutes.
5. Stop the reaction by adding the 800ul of 0.1N NaOH.
6. Record the absorbance at 405nm.

5.5 mM Ethylendiaminetetraacetic acid

500)

50mM NaCl

63mM sodium phosphate

655077)
- Fluorescent plate reader (PerkinElmer

66 mM Cytochrom-C

ELISA (0.5 µg/ml).
Western blot.

ELISA and Western blot.
Using an antigen Capture ELISA

ELISA.
Western blot.

ELISA.
Western Blot.
Lateral Flow.

ELISA.
Suitable for use as a Control or standard in indirect trapping ELISA for quantitation of antigen in serum using a standard curve.
Immunoprecipitation.
Western blotting.

ELISA.
Western blot: 1/1000.
Colloidal Gold and Latex Beads.

ELISA: this protein is suitable for use as a Mouse IgE standard in ELISA assays.

Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoassays.
Immunization.
Protocol: Reconstitution to filaments is performed by mixing equimolar amounts of keratins of type I and type II at concentrations of approx. 0.5 mg/ml

RIA.
Western blot.

Western Blot Control.
ATPase activity.
This protein has ATPase activity at the time of manufacture of 2.3 µM Phosphate liberated/hr/µg protein in a 200 µl reaction at 37°C (pH 8) in the presence of 20 µl of 1mM ATP using Malachite Green Assay.
Binding Assay.
ELISA reference standard.

Antigen in ELISA and Western blots.

Apo AI can be renatured into PBS

BML-SE211)
- 96 Well Polystyrene Microplate

Cat.No

Citric acid 0.055 g

Colloidal Gold and Western Blot.
Not recommended for Latex Beads.

D - glucose 2.05g in DW100 ml)
2. Centrifuge at 15000 rpm for 10 minutes and wash four times with PBS.
3. Dilute packed cells in a 0.5 mg/ml trypsin - EDTA solution to give 4% red cell suspension.
4. Incubate for 1h at 37°C and wash four times with PBS.
5 . Dilute packed cells in PBS to give 4% red cell suspension.
6 . Load 50ul of 0.5% BSA - in - 0.15M - NaCl solution and 25ul of 4% Red Cell - in - PBS in u shaped wells.
7 . Add 25ul of serial diluted galectin protein in PBS to each well plate . (Round bottom 96 well plate)
8 . Incubate for 30min at room temperature to observe visible agglutination.

ELISA (1/20

ELISA (1/8

ELISA and Western blot Immunoassay

ELISA and Western blot.
Glycosylation is heterogeneous and results in several immunoreactive products on Western blots that range from approximately 40

ELISA. Can be used for all immunological/laboratory methods requiring highly purified antigens.

ELISA.
Western blot.

ELISA: 0.4-0.8 µg/well.
After thawing

For use as a Control or standard in indirect trapping ELISA for quantitation of antigen in serum using a standard curve.
Immunoprecipitation.
Western blotting.

Protocol: 1. Prepare a 100ul of recombinant PGP9.5 protein with various concentrations (0.48ng

Protocol: Activity Assay
1. Prepare a 180 µl assay buffer into a suitable container and pre-chill on ice before use: The concentrations are 54 mM Potassium Phosphate

Protocol: Activity Assay
1. Prepare a 1.5 ml reaction mix into a suitable container

Protocol: Activity Assay
1. Prepare a 200 ul reaction mix into a suitable container : The final concentrations are 10mM Tris (pH 7.5)

Protocol: Activity Assay
1. Prepare 170 ul assay buffer into a suitable container and pre-chill on ice before use:
The final concentrations are 200 mM Tris-HCl

Protocol: Biological Assay :
1. Mix equal volumes of human blood and Alsever’s solution (pH 7.0). (Alsever’s solution: NaCl 0.42 g

Protocol: Activity Assay
1. Prepare a 1 ml reaction cocktail into a suitable container: The final concentrations are 5mM Tris

Second order rate constants for inhibition of:
uPA = 6.3 x 10e6 M-1s-1
tPA = 5.7 x 10e6 M-1s-1

Should be sonicated immediately prior to use to ensure that the preparation is uniform. Used for both IgG and IgM detection in assays which include EIA with polystyrene and latex solid phases and M capture formats.

Sodium citric aci d 0.8g

Specific methodologies have not been tested using this product.

Suitable for ELISA

Suitable for ELISA.

Suitable for Immunoblotting (Western or Dot blot

Suitable for immunoblotting (Western: 1/10

Suitable for use as a positive control in Lateral Flow assays and when spiked into stool samples.

Suitable for use in EIA and Western Blot. Each laboratory should determine an optimum working titer for
use in its particular application.

Suitable for use in ELISA and Western blot

Suitable for use in ELISA and Western blot immunoassay.

Suitable for use in ELISA and other phase Immunoassays.
BIN008 is typically required for IgM antibody testing.

Suitable for use in Western blot.

Suitable in ELISA.

Suitable to promote the adhesion and propagation of cells in vitro when used to coat cell culture surfaces

TBS and other common buffers by dialysis using a membrane with a nominal molecular weight cut-off of 14

Tested as solid-phase coating antigen in ELISA with anti-HBsAg antibodies.

Tested in ELISA with anti-HBsAg antibodies.

The DYKDDDDK peptide (Flag) is used as a fusion tag for immunoaffinity chromatography.

The antigen preparation should be sonicated immediately prior to use to ensure that the preparation is uniform. Grade 2 antigen is suitable for use for both IgG and IgM detection in assays which include EIA with polystyrene and latex solid phases. This preparation may be further purified to meet the needs of a particular assay format.

This preparation may be used as is in a variety of immunoassay formats or may be further purified to meet the needs of a particular assay format. May be used for both IgG and IgM detection in assays which include EIA with polystyrene and latex solid phases.

VICTOR X3)

Variety of Immunoassay formats including Microwell ELISA.
This product should be sonicated immediately prior to use to ensure that the preparation is uniform.

Western Blot and ELISA.

adjust to pH 7.5 at 37°C and pre-chill on ice before use: The final concentrations are 100 mM Sodium phosphate

and 20nM chymotrypsin.
2. Add 10 ul of recombinant Cyclophilin A protein with 1 ug in assay buffer.
3. Mix by inversion and equilibrate to 1°C and monitor the A405nm until the value is constant using a spectrophotometer.
4. Add 20 ul pre-chilled 5mM suc-AAFP-pNA. (Substrate was dissolved in TFE that contained 460mM LiCl to a concentration of 3 mM)
5. Record the increase in A405 nm for 30 minutes at 25°C.

black (greiner bio-one

both dissolved in 9.5 M urea buffer (see above). Protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4 M urea and then to low salt condition (50 mM NaCl

calibrator in immunoassays and in vitro studies.

calibrator in immunoassays and in vitro studies.

completely thaw the tube at 37°C without agitation

e.g. Dulbecco's PBS).

each lot is tested against a reference material for both IGM and IgG activity.

flicking or mixing

glassware and microcarrier beads. After spin and opening of the vial

immunogen for antibody production. Has been tested with anti-influenza A monoclonal antibodies in ELISA.

immunogen for antibody production. Has been tested with anti-influenza B monoclonal antibodies in ELISA.

immunogen for antibody production. Has been tested with anti-influenza B monoclonal antibodies in ELISA.

immunoperoxidase electron microscopy and immunochemistry (1/1

including plasticware

mix thoroughly by vortexing before use!

pH 7.4).
For immunization purposes

pH 8.0

pH 8.0.)

2. Add 50ul of 1uM Ubiquitin-AMC.

3. Read at excitation wavelengths 355nm and emission 460nm for 5 minutes.
- Ubiquitin-AMC (Enzo

reduced form

the solution can be further dialyzed against PBS (phosphate buffered saline

then gently mix the contents with a pipette.

Jak-STAT signaling pathway

Pathways in cancer

Notch signaling pathway

Chronic myeloid leukemia

ECM-receptor interaction

Hematopoietic cell lineage

Cell cycle

Acute myeloid leukemia

Cytokine-cytokine receptor interaction

Prostate cancer

Small cell lung cancer

p53 signaling pathway

Bladder cancer

Colorectal cancer

Endometrial cancer

Focal adhesion

Glioma

Melanoma

Non-small cell lung cancer

Pancreatic cancer

Thyroid cancer

Viral myocarditis

Wnt signaling pathway

Basal cell carcinoma

Hedgehog signaling pathway

Maturity onset diabetes of the young

ErbB signaling pathway

Huntington's disease

Oocyte meiosis

Dorso-ventral axis formation

Prion diseases

Human

E. coli

Human 293

1

2

Yes

In Stock