pLenti-C-Myc-DDK-P2A-Puro-mGFP, Lenti vector with C-terminal Myc-DDK tag, P2A-Puro selection marker and mGFP reporter gene
C-Tag | Myc-DDK |
E. coli Selection | Chloramphenicol (34 ug/mL) |
Cell Selection | Puromycin |
pLenti-C-Myc-DDK-P2A-Puro-mGFP, Lenti vector with C-terminal Myc-DDK tag, P2A-Puro selection marker and mGFP reporter gene
C-Tag | Myc-DDK |
E. coli Selection | Chloramphenicol (34 ug/mL) |
Cell Selection | Puromycin |
pCas-Guide-Nickase vector for gRNA cloning (with Cas9 H840A expression) (10 µg)
Function | Cas9 Nickase |
29-mer Scrambled shRNA Cassette in p-RFP-C-shLenti Vector, 5 ug
Viral Packaging | Lentiviral |
HuSH shRNA Lenti Cloning Vector (pC-shLenti Vector), 5 ug
Viral Packaging | Lentiviral |
pLenti-Cas9-Myc-DDK-P2A-Puro control plasmid containing the expression cassette for Cas9-Myc-DDK fusion protein and Puromycin resistance.
Function | Cas9 only vectors |
pCMV6-AC-mRFP, mammalian vector with C-terminal mRFP, 10ug
C-Tag | mRFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Cell Selection | Puromycin |
pCMV6-AC-mGFP-Puro, mammalian vector with C-terminal mGFP, 10ug
C-Tag | mGFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Cell Selection | Puromycin |
Catalytically inactive Cas9 (D10A/H840A) vector for gRNA cloning (10 µg)
Function | Cas9 Nickase |
pLenti-EF1a-C-Myc-DDK-IRES-BSD, Lenti vector with bicistronic IRES-BSD, 10ug
C-Tag | Myc-DDK |
E. coli Selection | Chloramphenicol (34 ug/mL) |
Cell Selection | Blasticidin |
29-mer Scrambled shRNA Cassette in pGFP-A-shAAV Vector, 5ug
Viral Packaging | Adeno-associated virus (AAV) |
Validated sgRNA targeting RFP dead mutant in GE100041C cells
29-mer Scrambled shRNA Cassette in pB-RS Vector, 5 ug
Viral Packaging | Retroviral |