pLenti-C-HA-DDK-P2A-Puro, Lenti vector with C-terminal HA-DDK tag and P2A-Puro, 10 ug
C-Tag | HA-DDK |
E. coli Selection | Chloramphenicol (34 ug/mL) |
Cell Selection | Puromycin |
pLenti-C-HA-DDK-P2A-Puro, Lenti vector with C-terminal HA-DDK tag and P2A-Puro, 10 ug
C-Tag | HA-DDK |
E. coli Selection | Chloramphenicol (34 ug/mL) |
Cell Selection | Puromycin |
pLenti-EF1a-Cas9-IRES-Puro, Cas9 in lenti vector under EF1a promoter with bicistronic IRES-Puro (10 ug)
Function | Cas9 only vectors |
pEX-1, bacterial expression vector with no tags, 10ug
E. coli Selection | Ampicillin (100 ug/mL) |
pCas-Guide-CRISPRi vector, All-in-one Vector for CRISPR/Cas9 Gene Interference with dCas9-KRAB-MeCP2 and gRNA cloning site.
Function | All-in-one CRISPRi vector |
pAAV-AC-GFP, AAV vector with C-terminal tGFP tag and EF1a promoter
ROSA26 donor vector with ROSA26 homologous arms cloned (Puro selection), ready for exogenous gene cloning (10 ug)
Function | ROSA26 donor vector |
pCas-Guide-GFP-CRISPRa vector, All-in-one Vector for CRISPR/Cas9 Gene activation with tGFP reporter, dCas9-VP64 and gRNA cloning site.
Function | All-in-one CRISPRa SAM vector |
pCMV6-Entry vector containing tGFP reporter gene
C-Tag | Myc-DDK |
E. coli Selection | Kanamycin (25 ug/mL) |
Cell Selection | Neomycin |
pLenti-N-DDK-P2A-Puro, Lenti vector with N-terminal DDK tag with P2A-Puro
N-Tag | DDK |
E. coli Selection | Chloramphenicol (34 ug/mL) |
Cell Selection | Puromycin |
pLenti-Cas9-IRES-Puro, Cas9 in lenti vector with bicistronic IRES-Puro (10 ug)
Function | Cas9 only vectors |
ROSA26 safe harbor gRNA vector, validated ROSA26 targeting sequence cloned in pCas-Guide vector
Function | Rosa26 gRNA |
pAAV-AC-RFP, AAV vector with C-terminal tRFP tag
pLenti-N-tYFP, Lenti Vector with N-terminal tYFP tag, 10ug
N-Tag | tYFP |
E. coli Selection | Chloramphenicol (34 ug/mL) |
pCMV6-AN-His-Myc, mammalian vector with N-terminal Myc-His tag, 10ug
N-Tag | His-Myc |
E. coli Selection | Ampicillin (100 ug/mL) |
Cell Selection | Neomycin |
pCas-Guide-Puro-CRISPRi Vector, All-in-one Vector for CRISPR/Cas9 Gene Interference with Puro selection marker, dCas9-KRAB-MeCP2 and gRNA cloning site.